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Biological activities of methanolic extract of selected local mushrooms

Local selected mushrooms; Ganoderma boninense, Auricularia auricula judae,Pleurotus cystidiosus and one new unidentified (BS01) were evaluated for the antioxidant, antimicrobial, anti-tyrosinase, anti-hyaluronidase, anti-inflammatory and insulin secretion activities. The antioxidant activity was mea...

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Main Author: Hashim, Mahfuzatunajla
Format: Thesis
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/48332/7/FBSB%202012%2053R.pdf
http://psasir.upm.edu.my/id/eprint/48332/
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spelling my.upm.eprints.483322016-11-04T04:51:08Z http://psasir.upm.edu.my/id/eprint/48332/ Biological activities of methanolic extract of selected local mushrooms Hashim, Mahfuzatunajla Local selected mushrooms; Ganoderma boninense, Auricularia auricula judae,Pleurotus cystidiosus and one new unidentified (BS01) were evaluated for the antioxidant, antimicrobial, anti-tyrosinase, anti-hyaluronidase, anti-inflammatory and insulin secretion activities. The antioxidant activity was measured using the DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity assay and ferric reducing antioxidant power assay (FRAP). In antioxidant activity, both G.boninense and A. A. judae showed the highest activity for DPPH and FRAP assays with the lowest IC50 value. The IC50 of G. boninense and A. A. judae for DPPH were 129.8 ± 1.8 and 198.5 ± 1.5 while for FRAP were 25.8 ± 5.0 and 52.7 ± 3.8,respectively. Anti-inflammatory activity was determined by inhibition of nitric oxide (NO) and measuring the nitrite (NO2 -) formation using Griess assay. However, only G. boninense showed inhibitory effect of NO inhibition with IC50 value at 151.3 μg/mL but the extract was also toxic to the RAW 264.7 cell at 500 μg/mL with cell viability percentage of 39.28 ± 2.5% only. Tyrosinase inhibitory was determined by a spectrophotometric method using L-3,4-ihydroxyphenylalanine (L-DOPA) as a substrate. BS01 exhibited significant (p < 0.05) inhibition with the IC50 value at 279.4 μg/mL and G. boninese at 474.4 μg/mL. The colorimetric Morgan-Elson method was carried out for hyaluronidase assay but all of the mushrooms were tested negative. Insulin secretion activity was measured using rat pancreatic β-cell line, BRIN-BD11 cells and the insulin level produced by the cell line was measured by an enzyme-linked immunosorbent assay using a commercial rat insulin ELISA. Among the mushrooms, G. boninense and P.cystidiosus extracts showed significant (p < 0.05) increased in insulin secretion at the concentration of 62.5, 125, 250 and 500 μg/mL. Overall, from the four mushrooms tested, G. boninense seem to exhibit more bioactive compounds and the further work could be done on the isolation, characterization and purification of the active compounds from the crude extract. 2012-08 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48332/7/FBSB%202012%2053R.pdf Hashim, Mahfuzatunajla (2012) Biological activities of methanolic extract of selected local mushrooms. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Local selected mushrooms; Ganoderma boninense, Auricularia auricula judae,Pleurotus cystidiosus and one new unidentified (BS01) were evaluated for the antioxidant, antimicrobial, anti-tyrosinase, anti-hyaluronidase, anti-inflammatory and insulin secretion activities. The antioxidant activity was measured using the DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity assay and ferric reducing antioxidant power assay (FRAP). In antioxidant activity, both G.boninense and A. A. judae showed the highest activity for DPPH and FRAP assays with the lowest IC50 value. The IC50 of G. boninense and A. A. judae for DPPH were 129.8 ± 1.8 and 198.5 ± 1.5 while for FRAP were 25.8 ± 5.0 and 52.7 ± 3.8,respectively. Anti-inflammatory activity was determined by inhibition of nitric oxide (NO) and measuring the nitrite (NO2 -) formation using Griess assay. However, only G. boninense showed inhibitory effect of NO inhibition with IC50 value at 151.3 μg/mL but the extract was also toxic to the RAW 264.7 cell at 500 μg/mL with cell viability percentage of 39.28 ± 2.5% only. Tyrosinase inhibitory was determined by a spectrophotometric method using L-3,4-ihydroxyphenylalanine (L-DOPA) as a substrate. BS01 exhibited significant (p < 0.05) inhibition with the IC50 value at 279.4 μg/mL and G. boninese at 474.4 μg/mL. The colorimetric Morgan-Elson method was carried out for hyaluronidase assay but all of the mushrooms were tested negative. Insulin secretion activity was measured using rat pancreatic β-cell line, BRIN-BD11 cells and the insulin level produced by the cell line was measured by an enzyme-linked immunosorbent assay using a commercial rat insulin ELISA. Among the mushrooms, G. boninense and P.cystidiosus extracts showed significant (p < 0.05) increased in insulin secretion at the concentration of 62.5, 125, 250 and 500 μg/mL. Overall, from the four mushrooms tested, G. boninense seem to exhibit more bioactive compounds and the further work could be done on the isolation, characterization and purification of the active compounds from the crude extract.
format Thesis
author Hashim, Mahfuzatunajla
spellingShingle Hashim, Mahfuzatunajla
Biological activities of methanolic extract of selected local mushrooms
author_facet Hashim, Mahfuzatunajla
author_sort Hashim, Mahfuzatunajla
title Biological activities of methanolic extract of selected local mushrooms
title_short Biological activities of methanolic extract of selected local mushrooms
title_full Biological activities of methanolic extract of selected local mushrooms
title_fullStr Biological activities of methanolic extract of selected local mushrooms
title_full_unstemmed Biological activities of methanolic extract of selected local mushrooms
title_sort biological activities of methanolic extract of selected local mushrooms
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/48332/7/FBSB%202012%2053R.pdf
http://psasir.upm.edu.my/id/eprint/48332/
_version_ 1643834144199802880
score 13.211869

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