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Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro

Background: The kava-kava plant (Piper methysticum) is traditionally consumed by the pacific islanders and has been linked to be involved in several biological activities. Flavokawain B is a unique chalcone, which can be found in the roots of the kava-kava plant. In this study, the operational mecha...

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Main Authors: Abu, Nadiah, Akhtar, Muhammad Nadeem, Yeap, Swee Keong, Lim, Kian Lam, Ho, Wan Yong, Abdullah, Mohd Puad, Ho, Chai Ling, Omar, Abdul Rahman, Ismail, Jamil, Mohammed Alitheen, Noorjahan Banu
Format: Article
Language:English
Published: BioMed Central 2016
Online Access:http://psasir.upm.edu.my/id/eprint/47474/1/47474.pdf
http://psasir.upm.edu.my/id/eprint/47474/
http://link.springer.com/article/10.1186/s12906-016-1046-8?view=classic
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spelling my.upm.eprints.474742016-05-20T01:53:39Z http://psasir.upm.edu.my/id/eprint/47474/ Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro Abu, Nadiah Akhtar, Muhammad Nadeem Yeap, Swee Keong Lim, Kian Lam Ho, Wan Yong Abdullah, Mohd Puad Ho, Chai Ling Omar, Abdul Rahman Ismail, Jamil Mohammed Alitheen, Noorjahan Banu Background: The kava-kava plant (Piper methysticum) is traditionally consumed by the pacific islanders and has been linked to be involved in several biological activities. Flavokawain B is a unique chalcone, which can be found in the roots of the kava-kava plant. In this study, the operational mechanism of the anti-cancer activity of a synthetic Flavokawain B (FKB) on two breast cancer cell lines, MCF-7 and MDA-MB231 was investigated. Method: Several in vitro assays were attempted such as MTT, flow cytometry of cell cycle analysis, annexin V analysis, and JC-1 analysis to detect apoptosis. Moreover, in vitro metastasis assays were also performed such as transwell migration assay, invasion assay, rat aorta ring and HUVEC tube formation. Molecular analysis of related genes and proteins were conducted using real-time PCR and proteome profiler analysis. Results: Based on our results, apoptosis was induced when both MCF-7 and MDA-MB231 were treated with FKB. A significant G2/M arrest was seen in MDA-MB231 cells. Additionally, FKB also inhibited the in vitro migration and invasion in MDA-MB231 cells in a dose dependent manner. Moreover, FKB can be a potential inhibitor in angiogenesis as it suppressed the formation of vessels in HUVEC cells as well as in the ex-vivo rat aortic ring assay. Conclusion: Our findings suggested that FKB also regulated several receptor tyrosine kinases. Overall, FKB is not only a potential candidate to be an anti-cancer agent, but as an anti-metastatic agent as well. BioMed Central 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/47474/1/47474.pdf Abu, Nadiah and Akhtar, Muhammad Nadeem and Yeap, Swee Keong and Lim, Kian Lam and Ho, Wan Yong and Abdullah, Mohd Puad and Ho, Chai Ling and Omar, Abdul Rahman and Ismail, Jamil and Mohammed Alitheen, Noorjahan Banu (2016) Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro. BMC Complementary and Alternative Medicine, 16 (1). art. no. 86. pp. 1-14. ISSN 1472-6882 http://link.springer.com/article/10.1186/s12906-016-1046-8?view=classic 10.1186/s12906-016-1046-8
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: The kava-kava plant (Piper methysticum) is traditionally consumed by the pacific islanders and has been linked to be involved in several biological activities. Flavokawain B is a unique chalcone, which can be found in the roots of the kava-kava plant. In this study, the operational mechanism of the anti-cancer activity of a synthetic Flavokawain B (FKB) on two breast cancer cell lines, MCF-7 and MDA-MB231 was investigated. Method: Several in vitro assays were attempted such as MTT, flow cytometry of cell cycle analysis, annexin V analysis, and JC-1 analysis to detect apoptosis. Moreover, in vitro metastasis assays were also performed such as transwell migration assay, invasion assay, rat aorta ring and HUVEC tube formation. Molecular analysis of related genes and proteins were conducted using real-time PCR and proteome profiler analysis. Results: Based on our results, apoptosis was induced when both MCF-7 and MDA-MB231 were treated with FKB. A significant G2/M arrest was seen in MDA-MB231 cells. Additionally, FKB also inhibited the in vitro migration and invasion in MDA-MB231 cells in a dose dependent manner. Moreover, FKB can be a potential inhibitor in angiogenesis as it suppressed the formation of vessels in HUVEC cells as well as in the ex-vivo rat aortic ring assay. Conclusion: Our findings suggested that FKB also regulated several receptor tyrosine kinases. Overall, FKB is not only a potential candidate to be an anti-cancer agent, but as an anti-metastatic agent as well.
format Article
author Abu, Nadiah
Akhtar, Muhammad Nadeem
Yeap, Swee Keong
Lim, Kian Lam
Ho, Wan Yong
Abdullah, Mohd Puad
Ho, Chai Ling
Omar, Abdul Rahman
Ismail, Jamil
Mohammed Alitheen, Noorjahan Banu
spellingShingle Abu, Nadiah
Akhtar, Muhammad Nadeem
Yeap, Swee Keong
Lim, Kian Lam
Ho, Wan Yong
Abdullah, Mohd Puad
Ho, Chai Ling
Omar, Abdul Rahman
Ismail, Jamil
Mohammed Alitheen, Noorjahan Banu
Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro
author_facet Abu, Nadiah
Akhtar, Muhammad Nadeem
Yeap, Swee Keong
Lim, Kian Lam
Ho, Wan Yong
Abdullah, Mohd Puad
Ho, Chai Ling
Omar, Abdul Rahman
Ismail, Jamil
Mohammed Alitheen, Noorjahan Banu
author_sort Abu, Nadiah
title Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro
title_short Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro
title_full Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro
title_fullStr Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro
title_full_unstemmed Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases in vitro
title_sort flavokawain b induced cytotoxicity in two breast cancer cell lines, mcf-7 and mda-mb231 and inhibited the metastatic potential of mda-mb231 via the regulation of several tyrosine kinases in vitro
publisher BioMed Central
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/47474/1/47474.pdf
http://psasir.upm.edu.my/id/eprint/47474/
http://link.springer.com/article/10.1186/s12906-016-1046-8?view=classic
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score 13.211869

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