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Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU

In a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine. The isolate was encapsulated in gellan gum and its ability to degrade caffeine was compared with the free cells. The optimal caffeine degra...

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Main Authors: Ibrahim, Salihu, Shukor, Mohd Yunus, Syed, Mohd Arif, Wan Johari, Wan Lutfi, Shamaan, Nor Aripin, Sabullah, Mohd Khalizan, Ahmad, Siti Aqlima
Format: Article
Language:English
Published: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 2016
Online Access:http://psasir.upm.edu.my/id/eprint/47471/1/Enhanced%20caffeine%20degradation%20by%20immobilised%20cells%20of%20Leifsonia%20sp.%20strain%20SIU.pdf
http://psasir.upm.edu.my/id/eprint/47471/
https://www.jstage.jst.go.jp/article/jgam/62/1/62_18/_article
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spelling my.upm.eprints.474712016-05-20T01:53:14Z http://psasir.upm.edu.my/id/eprint/47471/ Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU Ibrahim, Salihu Shukor, Mohd Yunus Syed, Mohd Arif Wan Johari, Wan Lutfi Shamaan, Nor Aripin Sabullah, Mohd Khalizan Ahmad, Siti Aqlima In a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine. The isolate was encapsulated in gellan gum and its ability to degrade caffeine was compared with the free cells. The optimal caffeine degradation was attained at a gellan gum concentration of 0.75% (w/v), a bead size of 4 mm diameter, and 250 beads per 100 mL of medium. At a caffeine concentration of 0.1 g/L, immobilised cells of the strain SIU degraded caffeine within 9 h, which is faster when compared to the case of free cells, in which it took 12 h to degrade. The immobilised cells degraded caffeine completely within 39 and 78 h at 0.5 and 1.0 g/L, while the free cells took 72 and 148 h at 0.5 and 1.0 g/L, respectively. At higher caffeine concentrations, immobilised cells exhibited a higher caffeine degradation rate. At concentrations of 1.5 and 2.0 g/L, caffeine-degrading activities of both immobilised and free cells were inhibited. The immobilised cells showed no loss in caffeine-degrading activity after being used repeatedly for nine 24-h cycles. The effect of heavy metals on immobilised cells was also tested. This study showed an increase in caffeine degradation efficiency when the cells were encapsulated in gellan gum. Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/47471/1/Enhanced%20caffeine%20degradation%20by%20immobilised%20cells%20of%20Leifsonia%20sp.%20strain%20SIU.pdf Ibrahim, Salihu and Shukor, Mohd Yunus and Syed, Mohd Arif and Wan Johari, Wan Lutfi and Shamaan, Nor Aripin and Sabullah, Mohd Khalizan and Ahmad, Siti Aqlima (2016) Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU. Journal of General and Applied Microbiology, 62 (1). pp. 18-24. ISSN 0022-1260; ESSN: 1349-8037 https://www.jstage.jst.go.jp/article/jgam/62/1/62_18/_article 10.2323/jgam.62.18
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description In a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine. The isolate was encapsulated in gellan gum and its ability to degrade caffeine was compared with the free cells. The optimal caffeine degradation was attained at a gellan gum concentration of 0.75% (w/v), a bead size of 4 mm diameter, and 250 beads per 100 mL of medium. At a caffeine concentration of 0.1 g/L, immobilised cells of the strain SIU degraded caffeine within 9 h, which is faster when compared to the case of free cells, in which it took 12 h to degrade. The immobilised cells degraded caffeine completely within 39 and 78 h at 0.5 and 1.0 g/L, while the free cells took 72 and 148 h at 0.5 and 1.0 g/L, respectively. At higher caffeine concentrations, immobilised cells exhibited a higher caffeine degradation rate. At concentrations of 1.5 and 2.0 g/L, caffeine-degrading activities of both immobilised and free cells were inhibited. The immobilised cells showed no loss in caffeine-degrading activity after being used repeatedly for nine 24-h cycles. The effect of heavy metals on immobilised cells was also tested. This study showed an increase in caffeine degradation efficiency when the cells were encapsulated in gellan gum.
format Article
author Ibrahim, Salihu
Shukor, Mohd Yunus
Syed, Mohd Arif
Wan Johari, Wan Lutfi
Shamaan, Nor Aripin
Sabullah, Mohd Khalizan
Ahmad, Siti Aqlima
spellingShingle Ibrahim, Salihu
Shukor, Mohd Yunus
Syed, Mohd Arif
Wan Johari, Wan Lutfi
Shamaan, Nor Aripin
Sabullah, Mohd Khalizan
Ahmad, Siti Aqlima
Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU
author_facet Ibrahim, Salihu
Shukor, Mohd Yunus
Syed, Mohd Arif
Wan Johari, Wan Lutfi
Shamaan, Nor Aripin
Sabullah, Mohd Khalizan
Ahmad, Siti Aqlima
author_sort Ibrahim, Salihu
title Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU
title_short Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU
title_full Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU
title_fullStr Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU
title_full_unstemmed Enhanced caffeine degradation by immobilised cells of Leifsonia sp. strain SIU
title_sort enhanced caffeine degradation by immobilised cells of leifsonia sp. strain siu
publisher Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/47471/1/Enhanced%20caffeine%20degradation%20by%20immobilised%20cells%20of%20Leifsonia%20sp.%20strain%20SIU.pdf
http://psasir.upm.edu.my/id/eprint/47471/
https://www.jstage.jst.go.jp/article/jgam/62/1/62_18/_article
_version_ 1643833892509057024
score 13.211869

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