Evidence of Sibling Species in the Brown Planthopper Complex (Nilaparvata lugens) Detected from Short and Long Primer Random Amplified Polymorphic DNA Fingerprints
The inheritance of 31 amplicons from short and long primer RAPD was tested for segregating ratios in two families of the brown planthopper,Nilaparvata lugens, and they were found to be inherited in a simple Mendelian fashion. These markers could now be used in population genetics studies of N. luge...
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2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/4693/1/Evidence%20of%20Sibling%20Species%20in%20the%20Brown%20Planthopper%20Complex.pdf http://psasir.upm.edu.my/id/eprint/4693/ http://dx.doi.org/10.1007/s10528-008-9167-5 |
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my.upm.eprints.46932015-10-23T08:11:14Z http://psasir.upm.edu.my/id/eprint/4693/ Evidence of Sibling Species in the Brown Planthopper Complex (Nilaparvata lugens) Detected from Short and Long Primer Random Amplified Polymorphic DNA Fingerprints Md Abdul Latif, Tan, Soon Guan Mohd Yusoh, Omar Siraj, Siti Shapor The inheritance of 31 amplicons from short and long primer RAPD was tested for segregating ratios in two families of the brown planthopper,Nilaparvata lugens, and they were found to be inherited in a simple Mendelian fashion. These markers could now be used in population genetics studies of N. lugens. Ten populations of N. lugens were collected from five locations in Malaysia. Each location had two sympatric populations. Cluster and principal coordinate analyses based on genetic distance along with AMOVA revealed that the rice-infesting populations (with high esterase activity) at five localities clustered together as a group, and Leersia-infesting populations (with low esterase activity) at the same localities formed another distinct cluster. Two amplicons from primers OPD03 (0.65 kb) and peh#6 (1.0 kb) could be considered diagnostic bands, which were fixed in the Leersia-infesting populations. These results represent evidence of a sibling species in the N. lugens complex. 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/4693/1/Evidence%20of%20Sibling%20Species%20in%20the%20Brown%20Planthopper%20Complex.pdf Md Abdul Latif, and Tan, Soon Guan and Mohd Yusoh, Omar and Siraj, Siti Shapor (2008) Evidence of Sibling Species in the Brown Planthopper Complex (Nilaparvata lugens) Detected from Short and Long Primer Random Amplified Polymorphic DNA Fingerprints. Biochemical Genetics, 46 (7-8). pp. 520-537. ISSN 0006-2928 http://dx.doi.org/10.1007/s10528-008-9167-5 10.1007/s10528-008-9167-5 English |
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The inheritance of 31 amplicons from short and long primer RAPD was tested for segregating ratios in two families of the brown planthopper,Nilaparvata lugens, and they were found to be inherited in a simple Mendelian fashion. These markers could now be used in population genetics studies of
N. lugens. Ten populations of N. lugens were collected from five locations in Malaysia. Each location had two sympatric populations. Cluster and principal coordinate analyses based on genetic distance along with AMOVA revealed that
the rice-infesting populations (with high esterase activity) at five localities clustered together as a group, and Leersia-infesting populations (with low esterase activity) at the same localities formed another distinct cluster. Two amplicons from primers OPD03 (0.65 kb) and peh#6 (1.0 kb) could be considered diagnostic bands, which were fixed in the Leersia-infesting populations.
These results represent evidence of a sibling species in the N. lugens complex. |
| format |
Article |
| author |
Md Abdul Latif, Tan, Soon Guan Mohd Yusoh, Omar Siraj, Siti Shapor |
| spellingShingle |
Md Abdul Latif, Tan, Soon Guan Mohd Yusoh, Omar Siraj, Siti Shapor Evidence of Sibling Species in the Brown Planthopper Complex (Nilaparvata lugens) Detected from Short and Long Primer Random Amplified Polymorphic DNA Fingerprints |
| author_facet |
Md Abdul Latif, Tan, Soon Guan Mohd Yusoh, Omar Siraj, Siti Shapor |
| author_sort |
Md Abdul Latif, |
| title |
Evidence of Sibling Species in the Brown Planthopper
Complex (Nilaparvata lugens) Detected from Short
and Long Primer Random Amplified Polymorphic DNA
Fingerprints |
| title_short |
Evidence of Sibling Species in the Brown Planthopper
Complex (Nilaparvata lugens) Detected from Short
and Long Primer Random Amplified Polymorphic DNA
Fingerprints |
| title_full |
Evidence of Sibling Species in the Brown Planthopper
Complex (Nilaparvata lugens) Detected from Short
and Long Primer Random Amplified Polymorphic DNA
Fingerprints |
| title_fullStr |
Evidence of Sibling Species in the Brown Planthopper
Complex (Nilaparvata lugens) Detected from Short
and Long Primer Random Amplified Polymorphic DNA
Fingerprints |
| title_full_unstemmed |
Evidence of Sibling Species in the Brown Planthopper
Complex (Nilaparvata lugens) Detected from Short
and Long Primer Random Amplified Polymorphic DNA
Fingerprints |
| title_sort |
evidence of sibling species in the brown planthopper
complex (nilaparvata lugens) detected from short
and long primer random amplified polymorphic dna
fingerprints |
| publishDate |
2008 |
| url |
http://psasir.upm.edu.my/id/eprint/4693/1/Evidence%20of%20Sibling%20Species%20in%20the%20Brown%20Planthopper%20Complex.pdf http://psasir.upm.edu.my/id/eprint/4693/ http://dx.doi.org/10.1007/s10528-008-9167-5 |
| _version_ |
1643822974908760064 |
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13.211869 |