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Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application

This paper discussed on the preparation of natural CNF from kenaf bast fibre for the application as a support structure in enzyme immobilization. The treatments involved for this preparation were delignification, bleaching and high-intensity ultra-sonication process to obtain nanofibre with high cel...

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Main Authors: Sulaiman, Safwan, Mokhtar, Mohd Noriznan, Naim, Mohd Nazli, Baharuddin, Azhari Samsu, Mohd Salleh, Mohamad Amran, Sulaiman, Alawi
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Online Access:http://psasir.upm.edu.my/id/eprint/46419/1/Study%20on%20the%20preparation%20of%20cellulose%20nanofibre%20%28CNF%29%20from%20kenaf%20bast%20fibre%20for%20enzyme%20immobilization%20application.pdf
http://psasir.upm.edu.my/id/eprint/46419/
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spelling my.upm.eprints.464192018-02-27T08:52:15Z http://psasir.upm.edu.my/id/eprint/46419/ Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Baharuddin, Azhari Samsu Mohd Salleh, Mohamad Amran Sulaiman, Alawi This paper discussed on the preparation of natural CNF from kenaf bast fibre for the application as a support structure in enzyme immobilization. The treatments involved for this preparation were delignification, bleaching and high-intensity ultra-sonication process to obtain nanofibre with high cellulose content and less than 100 nm diameter. Chemical composition analysis showed the influence of each process treatment on cellulose content of raw bast fibre, bleached pulp fibre and CNF(63.67, 81.12 and 91.97%, respectively). By increasing the cellulose content and decreasing the size of cellulose fibre, it resulted in a greater number of –OH functional group on its surface that plays as important role in enzyme immobilization. FTIR spectroscopy confirms that the removal of lignin and hemicellulose from the fibre after the treatments, as well as its interaction with coupling agents and CGTase enzyme. About 62.10% of enzyme loading and 45.62% of its activity yield were obtained after immobilization. Enzymatic reaction of immobilized CGTase on CNF indicates about more than 60% relative production yield of α-CD was achieved and its reusability was able to retain about 67.0% from its initial activity after 8 cycles of reaction. Therefore, the CNF is a good potential as a support for enzyme immobilization. Universiti Kebangsaan Malaysia 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46419/1/Study%20on%20the%20preparation%20of%20cellulose%20nanofibre%20%28CNF%29%20from%20kenaf%20bast%20fibre%20for%20enzyme%20immobilization%20application.pdf Sulaiman, Safwan and Mokhtar, Mohd Noriznan and Naim, Mohd Nazli and Baharuddin, Azhari Samsu and Mohd Salleh, Mohamad Amran and Sulaiman, Alawi (2015) Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application. Sains Malaysiana, 44 (11). pp. 1541-1550. ISSN 0026-6039
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This paper discussed on the preparation of natural CNF from kenaf bast fibre for the application as a support structure in enzyme immobilization. The treatments involved for this preparation were delignification, bleaching and high-intensity ultra-sonication process to obtain nanofibre with high cellulose content and less than 100 nm diameter. Chemical composition analysis showed the influence of each process treatment on cellulose content of raw bast fibre, bleached pulp fibre and CNF(63.67, 81.12 and 91.97%, respectively). By increasing the cellulose content and decreasing the size of cellulose fibre, it resulted in a greater number of –OH functional group on its surface that plays as important role in enzyme immobilization. FTIR spectroscopy confirms that the removal of lignin and hemicellulose from the fibre after the treatments, as well as its interaction with coupling agents and CGTase enzyme. About 62.10% of enzyme loading and 45.62% of its activity yield were obtained after immobilization. Enzymatic reaction of immobilized CGTase on CNF indicates about more than 60% relative production yield of α-CD was achieved and its reusability was able to retain about 67.0% from its initial activity after 8 cycles of reaction. Therefore, the CNF is a good potential as a support for enzyme immobilization.
format Article
author Sulaiman, Safwan
Mokhtar, Mohd Noriznan
Naim, Mohd Nazli
Baharuddin, Azhari Samsu
Mohd Salleh, Mohamad Amran
Sulaiman, Alawi
spellingShingle Sulaiman, Safwan
Mokhtar, Mohd Noriznan
Naim, Mohd Nazli
Baharuddin, Azhari Samsu
Mohd Salleh, Mohamad Amran
Sulaiman, Alawi
Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
author_facet Sulaiman, Safwan
Mokhtar, Mohd Noriznan
Naim, Mohd Nazli
Baharuddin, Azhari Samsu
Mohd Salleh, Mohamad Amran
Sulaiman, Alawi
author_sort Sulaiman, Safwan
title Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_short Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_full Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_fullStr Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_full_unstemmed Study on the preparation of cellulose nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_sort study on the preparation of cellulose nanofibre (cnf) from kenaf bast fibre for enzyme immobilization application
publisher Universiti Kebangsaan Malaysia
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/46419/1/Study%20on%20the%20preparation%20of%20cellulose%20nanofibre%20%28CNF%29%20from%20kenaf%20bast%20fibre%20for%20enzyme%20immobilization%20application.pdf
http://psasir.upm.edu.my/id/eprint/46419/
_version_ 1643833759916621824
score 13.211869

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