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Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method

A suitable recovery process aimed at purifying the sophorolipids (SL) biosurfactant with eliminating of solvents was studied. Two routes of recovery strategies to extract the SL from the culture medium were investigated: (1) organic-solvent extraction, (2) non-solvent extraction via salting-out meth...

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Main Authors: Abdul Razak, Nurul Nadhirah, Abdullah, Norhafizah, Awang Biak, Dayang Radiah, Mohd Yatim, Abdul Rashid
Format: Article
Language:English
Published: Malaysian Palm Oil Board 2015
Online Access:http://psasir.upm.edu.my/id/eprint/46337/1/Solvent-less%20Approach%20for%20the%20recovery%20of%20palm-based%20sophorolipids%20biosurfactant%20via%20salting-out%20method_New.pdf
http://psasir.upm.edu.my/id/eprint/46337/
http://jopr.mpob.gov.my/solvent-less-approach-for-the-recovery-of-palm-based-sophorolipids-biosurfactant-via-salting-out-method/
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spelling my.upm.eprints.463372022-06-17T08:01:40Z http://psasir.upm.edu.my/id/eprint/46337/ Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method Abdul Razak, Nurul Nadhirah Abdullah, Norhafizah Awang Biak, Dayang Radiah Mohd Yatim, Abdul Rashid A suitable recovery process aimed at purifying the sophorolipids (SL) biosurfactant with eliminating of solvents was studied. Two routes of recovery strategies to extract the SL from the culture medium were investigated: (1) organic-solvent extraction, (2) non-solvent extraction via salting-out methods. The recovered SL produced by yeast culture Starmerella bombicola (ATCC 22214), supplemented with glucose and refined, bleached and deodorised (RBD) palm olein as the primary and secondary carbon sources, yielded in 53 g litre-1 and 99 g litre-1, respectively. The SL were produced as a mixture of lactonic and acidic forms, as qualitatively predicted by the appearance of seven spots on the thin layer chromatography plates. By high performance liquid chromatography (HPLC) analysis, salting-out via precipitation method successfully reduced acidic compounds and resulted in lactonic-rich SL in the final product in which the purity was approximately 75%. This was revealed by the identification of five major structures and diacetylated lactonic SL (688 g mol-1) as the dominant compound. In conclusion, salting-out is recommended as a potential downstream processing for recovery of palm-based SL. Malaysian Palm Oil Board 2015-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46337/1/Solvent-less%20Approach%20for%20the%20recovery%20of%20palm-based%20sophorolipids%20biosurfactant%20via%20salting-out%20method_New.pdf Abdul Razak, Nurul Nadhirah and Abdullah, Norhafizah and Awang Biak, Dayang Radiah and Mohd Yatim, Abdul Rashid (2015) Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method. Journal of Oil Palm Research, 27 (2). pp. 181-189. ISSN 1511-2780; ESSN: 2811-4701 http://jopr.mpob.gov.my/solvent-less-approach-for-the-recovery-of-palm-based-sophorolipids-biosurfactant-via-salting-out-method/
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A suitable recovery process aimed at purifying the sophorolipids (SL) biosurfactant with eliminating of solvents was studied. Two routes of recovery strategies to extract the SL from the culture medium were investigated: (1) organic-solvent extraction, (2) non-solvent extraction via salting-out methods. The recovered SL produced by yeast culture Starmerella bombicola (ATCC 22214), supplemented with glucose and refined, bleached and deodorised (RBD) palm olein as the primary and secondary carbon sources, yielded in 53 g litre-1 and 99 g litre-1, respectively. The SL were produced as a mixture of lactonic and acidic forms, as qualitatively predicted by the appearance of seven spots on the thin layer chromatography plates. By high performance liquid chromatography (HPLC) analysis, salting-out via precipitation method successfully reduced acidic compounds and resulted in lactonic-rich SL in the final product in which the purity was approximately 75%. This was revealed by the identification of five major structures and diacetylated lactonic SL (688 g mol-1) as the dominant compound. In conclusion, salting-out is recommended as a potential downstream processing for recovery of palm-based SL.
format Article
author Abdul Razak, Nurul Nadhirah
Abdullah, Norhafizah
Awang Biak, Dayang Radiah
Mohd Yatim, Abdul Rashid
spellingShingle Abdul Razak, Nurul Nadhirah
Abdullah, Norhafizah
Awang Biak, Dayang Radiah
Mohd Yatim, Abdul Rashid
Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method
author_facet Abdul Razak, Nurul Nadhirah
Abdullah, Norhafizah
Awang Biak, Dayang Radiah
Mohd Yatim, Abdul Rashid
author_sort Abdul Razak, Nurul Nadhirah
title Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method
title_short Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method
title_full Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method
title_fullStr Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method
title_full_unstemmed Solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method
title_sort solvent-less approach for the recovery of palm-based sophorolipids biosurfactant via salting-out method
publisher Malaysian Palm Oil Board
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/46337/1/Solvent-less%20Approach%20for%20the%20recovery%20of%20palm-based%20sophorolipids%20biosurfactant%20via%20salting-out%20method_New.pdf
http://psasir.upm.edu.my/id/eprint/46337/
http://jopr.mpob.gov.my/solvent-less-approach-for-the-recovery-of-palm-based-sophorolipids-biosurfactant-via-salting-out-method/
_version_ 1736835684091559936
score 13.211869

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