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Seroepidemiology of toxoplasmosis among people having close contact with animals

A cross-sectional study was conducted to determine the seroepidemiology of Toxoplasma infection and its risk association among people having close contact with animals. A total of 312 blood samples were collected from veterinary personnel (veterinarian, technicians, and students) and pet owners from...

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Main Authors: Mong, Brandon Guo-Jie, Che Mat Seri, Nurul Asma Anati, Kumar Sharma, Reuben Sunil, Andiappan, Hemah, Tan, Tian-Chye, Lim, Yvonne Ai-Lian, Nissapatorn, Veeranoot
Format: Article
Language:English
Published: Frontiers Media 2015
Online Access:http://psasir.upm.edu.my/id/eprint/46267/1/Seroepidemiology%20of%20toxoplasmosis%20among%20people%20having%20close%20contact%20with%20animals.pdf
http://psasir.upm.edu.my/id/eprint/46267/
https://www.frontiersin.org/articles/10.3389/fimmu.2015.00143/full
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spelling my.upm.eprints.462672022-06-17T01:55:26Z http://psasir.upm.edu.my/id/eprint/46267/ Seroepidemiology of toxoplasmosis among people having close contact with animals Mong, Brandon Guo-Jie Che Mat Seri, Nurul Asma Anati Kumar Sharma, Reuben Sunil Andiappan, Hemah Tan, Tian-Chye Lim, Yvonne Ai-Lian Nissapatorn, Veeranoot A cross-sectional study was conducted to determine the seroepidemiology of Toxoplasma infection and its risk association among people having close contact with animals. A total of 312 blood samples were collected from veterinary personnel (veterinarian, technicians, and students) and pet owners from veterinary clinics and hospitals in the area of Klang Valley, Malaysia. About 4 cc of blood samples drawn from agreed participants were processed for measurement of anti-Toxoplasma IgG and IgM antibodies as well as avidity test of Toxoplasma IgG by ELISA I, II, and III kits. Meanwhile, the demographic profiles and possible risk factors of these participants were also recorded in the standardized data collection sheets. Overall seroprevalence of toxoplasmosis was observed in 62 (19.9%) participants being 7 (18.4%) in veterinarians, 15 (33.3%) in veterinary technicians, 29 (14.9%) in veterinary students, and 11 (31.4%) in pet owners. Of 19.9% Toxoplasma seropositive samples, 18.3% was positive for IgG antibody, 1.0% for IgM antibody, and 0.6% for both IgG and IgM antibodies. Of three different IgG avidity ELISA kits, ELISA III showed high avidity in all five seropositive samples (IgM and IgG/IgM antibodies) indicating chronic Toxoplasma infection which is consistent with no evidence of clinical toxoplasmosis diagnosed during the time of this study. Univariate analysis showed that age group, gender, study population, gardening, task performance, and working duration were significantly associated with Toxoplasma seropositivity. Further analysis by multivariate analysis using logistic regression showed that age group of ≥30 years old (OR = 0.34, 95% CI = 0.18–0.63, p = 0.001) and working or study duration of >10 years having close contact with animals (OR = 5.07, 95% CI = 1.80–14.24, p = 0.002) were identified as significant risks for Toxoplasma infection. Based on the results obtained, a comprehensive Toxoplasma screening and health surveillance program on toxoplasmosis should be implemented among people having close contact with animals in general and confirmed Toxoplasma seronegative individuals in particular to prevent seroconversion. Frontiers Media 2015-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46267/1/Seroepidemiology%20of%20toxoplasmosis%20among%20people%20having%20close%20contact%20with%20animals.pdf Mong, Brandon Guo-Jie and Che Mat Seri, Nurul Asma Anati and Kumar Sharma, Reuben Sunil and Andiappan, Hemah and Tan, Tian-Chye and Lim, Yvonne Ai-Lian and Nissapatorn, Veeranoot (2015) Seroepidemiology of toxoplasmosis among people having close contact with animals. Frontiers in immunology, 6. art. no. 143. pp. 1-6. ISSN 1664-3224 https://www.frontiersin.org/articles/10.3389/fimmu.2015.00143/full 10.3389/fimmu.2015.00143
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A cross-sectional study was conducted to determine the seroepidemiology of Toxoplasma infection and its risk association among people having close contact with animals. A total of 312 blood samples were collected from veterinary personnel (veterinarian, technicians, and students) and pet owners from veterinary clinics and hospitals in the area of Klang Valley, Malaysia. About 4 cc of blood samples drawn from agreed participants were processed for measurement of anti-Toxoplasma IgG and IgM antibodies as well as avidity test of Toxoplasma IgG by ELISA I, II, and III kits. Meanwhile, the demographic profiles and possible risk factors of these participants were also recorded in the standardized data collection sheets. Overall seroprevalence of toxoplasmosis was observed in 62 (19.9%) participants being 7 (18.4%) in veterinarians, 15 (33.3%) in veterinary technicians, 29 (14.9%) in veterinary students, and 11 (31.4%) in pet owners. Of 19.9% Toxoplasma seropositive samples, 18.3% was positive for IgG antibody, 1.0% for IgM antibody, and 0.6% for both IgG and IgM antibodies. Of three different IgG avidity ELISA kits, ELISA III showed high avidity in all five seropositive samples (IgM and IgG/IgM antibodies) indicating chronic Toxoplasma infection which is consistent with no evidence of clinical toxoplasmosis diagnosed during the time of this study. Univariate analysis showed that age group, gender, study population, gardening, task performance, and working duration were significantly associated with Toxoplasma seropositivity. Further analysis by multivariate analysis using logistic regression showed that age group of ≥30 years old (OR = 0.34, 95% CI = 0.18–0.63, p = 0.001) and working or study duration of >10 years having close contact with animals (OR = 5.07, 95% CI = 1.80–14.24, p = 0.002) were identified as significant risks for Toxoplasma infection. Based on the results obtained, a comprehensive Toxoplasma screening and health surveillance program on toxoplasmosis should be implemented among people having close contact with animals in general and confirmed Toxoplasma seronegative individuals in particular to prevent seroconversion.
format Article
author Mong, Brandon Guo-Jie
Che Mat Seri, Nurul Asma Anati
Kumar Sharma, Reuben Sunil
Andiappan, Hemah
Tan, Tian-Chye
Lim, Yvonne Ai-Lian
Nissapatorn, Veeranoot
spellingShingle Mong, Brandon Guo-Jie
Che Mat Seri, Nurul Asma Anati
Kumar Sharma, Reuben Sunil
Andiappan, Hemah
Tan, Tian-Chye
Lim, Yvonne Ai-Lian
Nissapatorn, Veeranoot
Seroepidemiology of toxoplasmosis among people having close contact with animals
author_facet Mong, Brandon Guo-Jie
Che Mat Seri, Nurul Asma Anati
Kumar Sharma, Reuben Sunil
Andiappan, Hemah
Tan, Tian-Chye
Lim, Yvonne Ai-Lian
Nissapatorn, Veeranoot
author_sort Mong, Brandon Guo-Jie
title Seroepidemiology of toxoplasmosis among people having close contact with animals
title_short Seroepidemiology of toxoplasmosis among people having close contact with animals
title_full Seroepidemiology of toxoplasmosis among people having close contact with animals
title_fullStr Seroepidemiology of toxoplasmosis among people having close contact with animals
title_full_unstemmed Seroepidemiology of toxoplasmosis among people having close contact with animals
title_sort seroepidemiology of toxoplasmosis among people having close contact with animals
publisher Frontiers Media
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/46267/1/Seroepidemiology%20of%20toxoplasmosis%20among%20people%20having%20close%20contact%20with%20animals.pdf
http://psasir.upm.edu.my/id/eprint/46267/
https://www.frontiersin.org/articles/10.3389/fimmu.2015.00143/full
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score 13.211869

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