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Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran

Background: Tea (Camellia sinensis), a well-known beverage is consumed frequently worldwide due to its high antioxidant properties. The present study determines the amount of phytochemicals and antioxidant activities among 12 high yielding tea clones cultivated in Iran. Results: Among the 12 clones...

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Main Authors: Sinniah, Uma Rani, Afzan, Adlin, Karimi, Ehsan, Mallappa, Kumara Swamy, Gonbad, Reza Azadi
Format: Article
Language:English
Published: Pontificia Universidad Catolica de Valparaiso 2015
Online Access:http://psasir.upm.edu.my/id/eprint/45870/1/FLAVANOID.pdf
http://psasir.upm.edu.my/id/eprint/45870/
https://scielo.conicyt.cl/pdf/ejb/v18n6/a08.pdf
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spelling my.upm.eprints.458702021-01-21T16:32:44Z http://psasir.upm.edu.my/id/eprint/45870/ Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran Sinniah, Uma Rani Afzan, Adlin Karimi, Ehsan Mallappa, Kumara Swamy Gonbad, Reza Azadi Background: Tea (Camellia sinensis), a well-known beverage is consumed frequently worldwide due to its high antioxidant properties. The present study determines the amount of phytochemicals and antioxidant activities among 12 high yielding tea clones cultivated in Iran. Results: Among the 12 clones studied, tea clone Iran 100 had the highest total phenolic content and total flavonoid content with values of 8.44 ± 1.03 mg gallic acid equivalents per gram dry weight and 4.50 ± 0.16 mg rutin equivalents per gram dry weight respectively. High performance Liquid Chromatography (HPLC) analysis of phenolics and flavonoids in 12 clones revealed the presence of (+)-catechin, (−)-epicatechin, (−)-epigallocatechin, (−)-epigallocatechin-gallate, (−)-epicatechingallate, gallic acid and caffeine. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay showed the existence of variation in the antioxidant activity ranging from 22.67 to 65.36%. The highest antioxidant activity with IC50 value of 218.24 μg/mL was observed in the leaf extract of the clone Iran 100, while the lowest was found in the clone Iran 482 with IC50 value of 234.44 μg/mL. The antioxidant activity had a positive correlation with total phenolic content, total flavonoid content, (−)-epigallocatechin-gallate, (−)-epicatechingallate and caffeine (0.59 ≤ r ≤ 0.97, P b 0.05). Conclusion: From the study it can be concluded that the clone Iran 100 has a superior quality compared to any other clones studied due to occurrence of more phenolic compounds and a greater antioxidant activity. Hence, we recommend the use of tea clone Iran 100 for commercial planting. Pontificia Universidad Catolica de Valparaiso 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45870/1/FLAVANOID.pdf Sinniah, Uma Rani and Afzan, Adlin and Karimi, Ehsan and Mallappa, Kumara Swamy and Gonbad, Reza Azadi (2015) Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran. Electronic Journal of Biotechnology, 18. pp. 433-438. ISSN 0717-3458 https://scielo.conicyt.cl/pdf/ejb/v18n6/a08.pdf 10.1016/j.ejbt.2015.08.007
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Tea (Camellia sinensis), a well-known beverage is consumed frequently worldwide due to its high antioxidant properties. The present study determines the amount of phytochemicals and antioxidant activities among 12 high yielding tea clones cultivated in Iran. Results: Among the 12 clones studied, tea clone Iran 100 had the highest total phenolic content and total flavonoid content with values of 8.44 ± 1.03 mg gallic acid equivalents per gram dry weight and 4.50 ± 0.16 mg rutin equivalents per gram dry weight respectively. High performance Liquid Chromatography (HPLC) analysis of phenolics and flavonoids in 12 clones revealed the presence of (+)-catechin, (−)-epicatechin, (−)-epigallocatechin, (−)-epigallocatechin-gallate, (−)-epicatechingallate, gallic acid and caffeine. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay showed the existence of variation in the antioxidant activity ranging from 22.67 to 65.36%. The highest antioxidant activity with IC50 value of 218.24 μg/mL was observed in the leaf extract of the clone Iran 100, while the lowest was found in the clone Iran 482 with IC50 value of 234.44 μg/mL. The antioxidant activity had a positive correlation with total phenolic content, total flavonoid content, (−)-epigallocatechin-gallate, (−)-epicatechingallate and caffeine (0.59 ≤ r ≤ 0.97, P b 0.05). Conclusion: From the study it can be concluded that the clone Iran 100 has a superior quality compared to any other clones studied due to occurrence of more phenolic compounds and a greater antioxidant activity. Hence, we recommend the use of tea clone Iran 100 for commercial planting.
format Article
author Sinniah, Uma Rani
Afzan, Adlin
Karimi, Ehsan
Mallappa, Kumara Swamy
Gonbad, Reza Azadi
spellingShingle Sinniah, Uma Rani
Afzan, Adlin
Karimi, Ehsan
Mallappa, Kumara Swamy
Gonbad, Reza Azadi
Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
author_facet Sinniah, Uma Rani
Afzan, Adlin
Karimi, Ehsan
Mallappa, Kumara Swamy
Gonbad, Reza Azadi
author_sort Sinniah, Uma Rani
title Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_short Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_full Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_fullStr Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_full_unstemmed Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_sort phytoconstituents and antioxidant properties among commercial tea (camellia sinensis l.) clones of iran
publisher Pontificia Universidad Catolica de Valparaiso
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/45870/1/FLAVANOID.pdf
http://psasir.upm.edu.my/id/eprint/45870/
https://scielo.conicyt.cl/pdf/ejb/v18n6/a08.pdf
_version_ 1690372562231492608
score 13.211869

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