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Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants

Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in b...

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Main Authors: Withanage, Samanthi Priyanka, Hossain, Md. Aktar, Muniandi, Sures Kumar, Roslan, Hairul Azman, Abdullah, Mohd Puad, Napis, Suhaimi, Ab Shukor, Nor Aini
Format: Article
Language:English
Published: Japanese Society of Breeding 2015
Online Access:http://psasir.upm.edu.my/id/eprint/45728/1/KENAF.pdf
http://psasir.upm.edu.my/id/eprint/45728/
https://www.jstage.jst.go.jp/article/jsbbs/65/3/65_177/_article/-char/en
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spelling my.upm.eprints.457282021-04-22T08:03:05Z http://psasir.upm.edu.my/id/eprint/45728/ Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants Withanage, Samanthi Priyanka Hossain, Md. Aktar Muniandi, Sures Kumar Roslan, Hairul Azman Abdullah, Mohd Puad Napis, Suhaimi Ab Shukor, Nor Aini Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants. Japanese Society of Breeding 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45728/1/KENAF.pdf Withanage, Samanthi Priyanka and Hossain, Md. Aktar and Muniandi, Sures Kumar and Roslan, Hairul Azman and Abdullah, Mohd Puad and Napis, Suhaimi and Ab Shukor, Nor Aini (2015) Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants. Breeding Science, 65 (3). pp. 177-191. ISSN 1344-7610; ESSN: 1347-3735 https://www.jstage.jst.go.jp/article/jsbbs/65/3/65_177/_article/-char/en 10.1270/jsbbs.65.177
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.
format Article
author Withanage, Samanthi Priyanka
Hossain, Md. Aktar
Muniandi, Sures Kumar
Roslan, Hairul Azman
Abdullah, Mohd Puad
Napis, Suhaimi
Ab Shukor, Nor Aini
spellingShingle Withanage, Samanthi Priyanka
Hossain, Md. Aktar
Muniandi, Sures Kumar
Roslan, Hairul Azman
Abdullah, Mohd Puad
Napis, Suhaimi
Ab Shukor, Nor Aini
Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants
author_facet Withanage, Samanthi Priyanka
Hossain, Md. Aktar
Muniandi, Sures Kumar
Roslan, Hairul Azman
Abdullah, Mohd Puad
Napis, Suhaimi
Ab Shukor, Nor Aini
author_sort Withanage, Samanthi Priyanka
title Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants
title_short Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants
title_full Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants
title_fullStr Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants
title_full_unstemmed Overexpression of Arabidopsis thaliana gibberelic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L) plants
title_sort overexpression of arabidopsis thaliana gibberelic acid 20 oxidase (atga20ox) gene enhance the vegetative growth and fiber quality in kenaf (hibiscus cannabinus l) plants
publisher Japanese Society of Breeding
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/45728/1/KENAF.pdf
http://psasir.upm.edu.my/id/eprint/45728/
https://www.jstage.jst.go.jp/article/jsbbs/65/3/65_177/_article/-char/en
_version_ 1698698920974090240
score 13.211869

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