Cover Image

Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis

Hemibagrus nemurus is a valuable cultured catfish, but the production is limited due to problems associated with its reproduction, which are controlled by two pituitary gonadotropin (GtH) hormones, follicle-stimulating hormone (FSH) and luteinizing hormone (LH). This study reports the cloning, seque...

Full description

Saved in:
Bibliographic Details
Main Authors: Md Yasin, Ina Salwany, Christianus, Annie, Md. Yusoff, Fatimah, Harmin, Sharr Azni
Format: Article
Language:English
Published: GKS Publishers 2015
Online Access:http://psasir.upm.edu.my/id/eprint/45519/1/DNA.pdf
http://psasir.upm.edu.my/id/eprint/45519/
https://updatepublishing.com/journal/index.php/rib/article/view/2656
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.45519
record_format eprints
spelling my.upm.eprints.455192021-01-23T21:54:08Z http://psasir.upm.edu.my/id/eprint/45519/ Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis Md Yasin, Ina Salwany Christianus, Annie Md. Yusoff, Fatimah Harmin, Sharr Azni Hemibagrus nemurus is a valuable cultured catfish, but the production is limited due to problems associated with its reproduction, which are controlled by two pituitary gonadotropin (GtH) hormones, follicle-stimulating hormone (FSH) and luteinizing hormone (LH). This study reports the cloning, sequence analysis, and phylogenetic study of GtH subunits from H. nemurus. This work revealed that the cDNAs of Gp-α, FSHβ and LHβ were 656, 728, and 602 nucleotides in length and encoded for mature peptides of 92, 117, and 115 amino acids, respectively. The amino acid sequence identities of H. nemurus mature subunits Gp-α, FSHβ and LHβ in comparison with other fishes were 51-100%, 23-89% and 44-88%, respectively; while in comparison with tetrapods were 64-73%, 12-30% and 26-52%, respectively. The cysteine residues of the mature protein were conserved in comparison with other fishes, which were 10, 13 and 12 cysteines in Gp-α, FSHβ and LHβ, respectively. Meanwhile, all potential N-linked glycosylation sites were fully conserved with other vertebrates. The Gp-α, FSHβ and LHβ of H. nemurus resembled most to those of Siluriformes, Cypriniformes, and Angulliformes implying a close phylogenetic relationship. This study demonstrates that Malaysian H. nemurus can be utilized as a good model for investigating GtH functions in other catfish species throughout the Indo-Pacific region. GKS Publishers 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45519/1/DNA.pdf Md Yasin, Ina Salwany and Christianus, Annie and Md. Yusoff, Fatimah and Harmin, Sharr Azni (2015) Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis. Research in Biotechnology, 6 (1). pp. 26-46. ISSN 2229-791X https://updatepublishing.com/journal/index.php/rib/article/view/2656
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Hemibagrus nemurus is a valuable cultured catfish, but the production is limited due to problems associated with its reproduction, which are controlled by two pituitary gonadotropin (GtH) hormones, follicle-stimulating hormone (FSH) and luteinizing hormone (LH). This study reports the cloning, sequence analysis, and phylogenetic study of GtH subunits from H. nemurus. This work revealed that the cDNAs of Gp-α, FSHβ and LHβ were 656, 728, and 602 nucleotides in length and encoded for mature peptides of 92, 117, and 115 amino acids, respectively. The amino acid sequence identities of H. nemurus mature subunits Gp-α, FSHβ and LHβ in comparison with other fishes were 51-100%, 23-89% and 44-88%, respectively; while in comparison with tetrapods were 64-73%, 12-30% and 26-52%, respectively. The cysteine residues of the mature protein were conserved in comparison with other fishes, which were 10, 13 and 12 cysteines in Gp-α, FSHβ and LHβ, respectively. Meanwhile, all potential N-linked glycosylation sites were fully conserved with other vertebrates. The Gp-α, FSHβ and LHβ of H. nemurus resembled most to those of Siluriformes, Cypriniformes, and Angulliformes implying a close phylogenetic relationship. This study demonstrates that Malaysian H. nemurus can be utilized as a good model for investigating GtH functions in other catfish species throughout the Indo-Pacific region.
format Article
author Md Yasin, Ina Salwany
Christianus, Annie
Md. Yusoff, Fatimah
Harmin, Sharr Azni
spellingShingle Md Yasin, Ina Salwany
Christianus, Annie
Md. Yusoff, Fatimah
Harmin, Sharr Azni
Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis
author_facet Md Yasin, Ina Salwany
Christianus, Annie
Md. Yusoff, Fatimah
Harmin, Sharr Azni
author_sort Md Yasin, Ina Salwany
title Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis
title_short Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis
title_full Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis
title_fullStr Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis
title_full_unstemmed Molecular characterization of Hemibagrus nemurus gonadotropin subunits: cDNA cloning and phylogenetic analysis
title_sort molecular characterization of hemibagrus nemurus gonadotropin subunits: cdna cloning and phylogenetic analysis
publisher GKS Publishers
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/45519/1/DNA.pdf
http://psasir.upm.edu.my/id/eprint/45519/
https://updatepublishing.com/journal/index.php/rib/article/view/2656
_version_ 1690372554388144128
score 13.211869

Similar Items