Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience

Purpose: This study was conducted to assess the agreement and differences between cervical self-sampling with a Kato device (KSSD) and gynecologist sampling for Pap cytology and human papillomavirus DNA (HPV DNA) detection. Materials and methods: Women underwent self-sampling followed by gynecologis...

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Main Authors: Abd Latiff, Latiffah, Ibrahim, Zaidah, Chong, Pei Pei, Abdul Rahman, Sabariah, Akhtari-Zavare, Mehrnoosh
Format: Article
Language:English
Published: Asian Pacific Organization for Cancer Prevention 2015
Online Access:http://psasir.upm.edu.my/id/eprint/44111/1/Comparative%20assessment%20of%20self-sampling%20device%20and%20gynecologist%20sampling%20for%20cytology%20and%20HPV%20DNA%20detection%20in%20rural%20and%20low%20resource%20setting%20A%20Malaysian%20experience.pdf
http://psasir.upm.edu.my/id/eprint/44111/
http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:26745108&key=2015.16.18.8495
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spelling my.upm.eprints.441112022-02-12T02:56:04Z http://psasir.upm.edu.my/id/eprint/44111/ Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience Abd Latiff, Latiffah Ibrahim, Zaidah Chong, Pei Pei Abdul Rahman, Sabariah Akhtari-Zavare, Mehrnoosh Purpose: This study was conducted to assess the agreement and differences between cervical self-sampling with a Kato device (KSSD) and gynecologist sampling for Pap cytology and human papillomavirus DNA (HPV DNA) detection. Materials and methods: Women underwent self-sampling followed by gynecologist sampling during screening at two primary health clinics. Pap cytology of cervical specimens was evaluated for specimen adequacy, presence of endocervical cells or transformation zone cells and cytological interpretation for cells abnormalities. Cervical specimens were also extracted and tested for HPV DNA detection. Positive HPV smears underwent gene sequencing and HPV genotyping by referring to the online NCBI gene bank. Results were compared between samplings by Kappa agreement and McNemar test. Results: For Pap specimen adequacy, KSSD showed 100% agreement with gynecologist sampling but had only 32.3% agreement for presence of endocervical cells. Both sampling showed 100% agreement with only 1 case detected HSIL favouring CIN2 for cytology result. HPV DNA detection showed 86.2%agreement (K=0.64, 95% CI 0.524-0.756, p=0.001) between samplings. KSSD and gynaecologist sampling identified high risk HPV in 17.3% and 23.9% respectively (p= 0.014). Conclusion: The self-sampling using Kato device can serve as a tool in Pap cytology and HPV DNA detection in low resource settings in Malaysia. Self-sampling devices such as KSSD can be used as an alternative technique to gynaecologist sampling for cervical cancer screening among rural populations in Malaysia. Asian Pacific Organization for Cancer Prevention 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/44111/1/Comparative%20assessment%20of%20self-sampling%20device%20and%20gynecologist%20sampling%20for%20cytology%20and%20HPV%20DNA%20detection%20in%20rural%20and%20low%20resource%20setting%20A%20Malaysian%20experience.pdf Abd Latiff, Latiffah and Ibrahim, Zaidah and Chong, Pei Pei and Abdul Rahman, Sabariah and Akhtari-Zavare, Mehrnoosh (2015) Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience. Asian Pacific Journal of Cancer Prevention, 16 (18). pp. 8495-8501. ISSN 1513-7368; ESSN: 2476-762X http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:26745108&key=2015.16.18.8495 10.7314/APJCP.2015.16.18.8495
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Purpose: This study was conducted to assess the agreement and differences between cervical self-sampling with a Kato device (KSSD) and gynecologist sampling for Pap cytology and human papillomavirus DNA (HPV DNA) detection. Materials and methods: Women underwent self-sampling followed by gynecologist sampling during screening at two primary health clinics. Pap cytology of cervical specimens was evaluated for specimen adequacy, presence of endocervical cells or transformation zone cells and cytological interpretation for cells abnormalities. Cervical specimens were also extracted and tested for HPV DNA detection. Positive HPV smears underwent gene sequencing and HPV genotyping by referring to the online NCBI gene bank. Results were compared between samplings by Kappa agreement and McNemar test. Results: For Pap specimen adequacy, KSSD showed 100% agreement with gynecologist sampling but had only 32.3% agreement for presence of endocervical cells. Both sampling showed 100% agreement with only 1 case detected HSIL favouring CIN2 for cytology result. HPV DNA detection showed 86.2%agreement (K=0.64, 95% CI 0.524-0.756, p=0.001) between samplings. KSSD and gynaecologist sampling identified high risk HPV in 17.3% and 23.9% respectively (p= 0.014). Conclusion: The self-sampling using Kato device can serve as a tool in Pap cytology and HPV DNA detection in low resource settings in Malaysia. Self-sampling devices such as KSSD can be used as an alternative technique to gynaecologist sampling for cervical cancer screening among rural populations in Malaysia.
format Article
author Abd Latiff, Latiffah
Ibrahim, Zaidah
Chong, Pei Pei
Abdul Rahman, Sabariah
Akhtari-Zavare, Mehrnoosh
spellingShingle Abd Latiff, Latiffah
Ibrahim, Zaidah
Chong, Pei Pei
Abdul Rahman, Sabariah
Akhtari-Zavare, Mehrnoosh
Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience
author_facet Abd Latiff, Latiffah
Ibrahim, Zaidah
Chong, Pei Pei
Abdul Rahman, Sabariah
Akhtari-Zavare, Mehrnoosh
author_sort Abd Latiff, Latiffah
title Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience
title_short Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience
title_full Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience
title_fullStr Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience
title_full_unstemmed Comparative assessment of self-sampling device and gynecologist sampling for cytology and HPV DNA detection in rural and low resource setting: Malaysian experience
title_sort comparative assessment of self-sampling device and gynecologist sampling for cytology and hpv dna detection in rural and low resource setting: malaysian experience
publisher Asian Pacific Organization for Cancer Prevention
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/44111/1/Comparative%20assessment%20of%20self-sampling%20device%20and%20gynecologist%20sampling%20for%20cytology%20and%20HPV%20DNA%20detection%20in%20rural%20and%20low%20resource%20setting%20A%20Malaysian%20experience.pdf
http://psasir.upm.edu.my/id/eprint/44111/
http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:26745108&key=2015.16.18.8495
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