Staff View: Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model

Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model

Cancer nanotherapy is progressing rapidly with the introduction of many innovative drug delivery systems to replace conventional therapy. Although the antitumor activity of zerumbone (ZER) has been reported, there has been no information available on the effect of ZER-loaded nanostructured lipid car...

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Main Authors:	Rahman, Heshu Sulaiman, Rasedee, Abdullah, Chee, Wun How, Zeenathul, Nazariah Allaudin, Chartrand, Max Stanley, Swee, Keong Yeap, Abdul, Ahmad Bustamam, Sheau, Wei Tan, Othman, Hemn Hassan, Ajdari, Zahra, Namvar, Farideh, Arulselvan, Palanisamy, Fakurazi, Sharida, Mehrbod, Parvaneh, Daneshvar, Nasibeh, Begum, Hasina
Format:	Article
Language:	English
Published:	Dove Medical Press 2015
Online Access:	http://psasir.upm.edu.my/id/eprint/43792/1/Antileukemic%20effect%20of%20zerumbone-loaded.pdf http://psasir.upm.edu.my/id/eprint/43792/
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spelling	my.upm.eprints.437922018-04-09T06:25:23Z http://psasir.upm.edu.my/id/eprint/43792/ Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model Rahman, Heshu Sulaiman Rasedee, Abdullah Chee, Wun How Zeenathul, Nazariah Allaudin Chartrand, Max Stanley Swee, Keong Yeap Abdul, Ahmad Bustamam Sheau, Wei Tan Othman, Hemn Hassan Ajdari, Zahra Namvar, Farideh Arulselvan, Palanisamy Fakurazi, Sharida Mehrbod, Parvaneh Daneshvar, Nasibeh Begum, Hasina Cancer nanotherapy is progressing rapidly with the introduction of many innovative drug delivery systems to replace conventional therapy. Although the antitumor activity of zerumbone (ZER) has been reported, there has been no information available on the effect of ZER-loaded nanostructured lipid carrier (NLC) (ZER-NLC) on murine leukemia cells. In this study, the in vitro and in vivo effects of ZER-NLC on murine leukemia induced with WEHI-3B cells were investigated. The results from 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Hoechst 33342, Annexin V, cell cycle, and caspase activity assays showed that the growth of leukemia cells in vitro was inhibited by ZER-NLC. In addition, outcomes of histopathology, transmission electron microscopy, and Tdt-mediated dUTP nick-end labeling analyses revealed that the number of leukemia cells in the spleen of BALB/c leukemia mice significantly decreased after 4 weeks of oral treatment with various doses of ZER-NLC. Western blotting and reverse-transcription quantitative polymerase chain reaction assays confirmed the antileukemia effects of ZER-NLC. In conclusion, ZER-NLC was shown to induce a mitochondrial-dependent apoptotic pathway in murine leukemia. Loading of ZER in NLC did not compromise the anticancer effect of the compound, suggesting ZER-NLC as a promising and effective delivery system for treatment of cancers. Dove Medical Press 2015-03-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/43792/1/Antileukemic%20effect%20of%20zerumbone-loaded.pdf Rahman, Heshu Sulaiman and Rasedee, Abdullah and Chee, Wun How and Zeenathul, Nazariah Allaudin and Chartrand, Max Stanley and Swee, Keong Yeap and Abdul, Ahmad Bustamam and Sheau, Wei Tan and Othman, Hemn Hassan and Ajdari, Zahra and Namvar, Farideh and Arulselvan, Palanisamy and Fakurazi, Sharida and Mehrbod, Parvaneh and Daneshvar, Nasibeh and Begum, Hasina (2015) Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model. International Journal of Nanomedicine, 10. pp. 1649-1666. ISSN 1176-9114; ESSN: 1178-2013 10.2147/IJN.S67113
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Cancer nanotherapy is progressing rapidly with the introduction of many innovative drug delivery systems to replace conventional therapy. Although the antitumor activity of zerumbone (ZER) has been reported, there has been no information available on the effect of ZER-loaded nanostructured lipid carrier (NLC) (ZER-NLC) on murine leukemia cells. In this study, the in vitro and in vivo effects of ZER-NLC on murine leukemia induced with WEHI-3B cells were investigated. The results from 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Hoechst 33342, Annexin V, cell cycle, and caspase activity assays showed that the growth of leukemia cells in vitro was inhibited by ZER-NLC. In addition, outcomes of histopathology, transmission electron microscopy, and Tdt-mediated dUTP nick-end labeling analyses revealed that the number of leukemia cells in the spleen of BALB/c leukemia mice significantly decreased after 4 weeks of oral treatment with various doses of ZER-NLC. Western blotting and reverse-transcription quantitative polymerase chain reaction assays confirmed the antileukemia effects of ZER-NLC. In conclusion, ZER-NLC was shown to induce a mitochondrial-dependent apoptotic pathway in murine leukemia. Loading of ZER in NLC did not compromise the anticancer effect of the compound, suggesting ZER-NLC as a promising and effective delivery system for treatment of cancers.
format	Article
author	Rahman, Heshu Sulaiman Rasedee, Abdullah Chee, Wun How Zeenathul, Nazariah Allaudin Chartrand, Max Stanley Swee, Keong Yeap Abdul, Ahmad Bustamam Sheau, Wei Tan Othman, Hemn Hassan Ajdari, Zahra Namvar, Farideh Arulselvan, Palanisamy Fakurazi, Sharida Mehrbod, Parvaneh Daneshvar, Nasibeh Begum, Hasina
spellingShingle	Rahman, Heshu Sulaiman Rasedee, Abdullah Chee, Wun How Zeenathul, Nazariah Allaudin Chartrand, Max Stanley Swee, Keong Yeap Abdul, Ahmad Bustamam Sheau, Wei Tan Othman, Hemn Hassan Ajdari, Zahra Namvar, Farideh Arulselvan, Palanisamy Fakurazi, Sharida Mehrbod, Parvaneh Daneshvar, Nasibeh Begum, Hasina Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model
author_facet	Rahman, Heshu Sulaiman Rasedee, Abdullah Chee, Wun How Zeenathul, Nazariah Allaudin Chartrand, Max Stanley Swee, Keong Yeap Abdul, Ahmad Bustamam Sheau, Wei Tan Othman, Hemn Hassan Ajdari, Zahra Namvar, Farideh Arulselvan, Palanisamy Fakurazi, Sharida Mehrbod, Parvaneh Daneshvar, Nasibeh Begum, Hasina
author_sort	Rahman, Heshu Sulaiman
title	Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model
title_short	Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model
title_full	Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model
title_fullStr	Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model
title_full_unstemmed	Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model
title_sort	antileukemic effect of zerumbone-loaded nanostructured lipid carrier in wehi-3b cell-induced murine leukemia model
publisher	Dove Medical Press
publishDate	2015
url	http://psasir.upm.edu.my/id/eprint/43792/1/Antileukemic%20effect%20of%20zerumbone-loaded.pdf http://psasir.upm.edu.my/id/eprint/43792/
_version_	1643833670378717184
score	13.211869

Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model

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