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A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain

Malayan box turtle (Cuora amboinensis) has been a wildlife-protected vulnerable turtle species in Malaysia since 2005. However, because of its purported usage in traditional medicine, tonic foods and feeds, clandestine black market trade is rampant. Several polymerase chain reaction (PCR) assays for...

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Main Authors: Ali, Md. Eaqub, Asing,, Abd Hamid, Sharifah Bee, Razzak, Md. Abdur, Abd Rashid, Nur Raifana, Al Amin, Md., Mustafa, Shuhaimi
Format: Article
Language:English
Published: Taylor & Francis Group 2015
Online Access:http://psasir.upm.edu.my/id/eprint/43613/1/A%20suitable%20method%20to%20detect%20potential%20fraud%20of%20bringing%20Malayan%20box%20turtle%20%28Cuora%20amboinensis%29%20meat%20into%20the%20food%20chain.pdf
http://psasir.upm.edu.my/id/eprint/43613/
http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1058535?journalCode=tfac20
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spelling my.upm.eprints.436132016-07-22T01:18:42Z http://psasir.upm.edu.my/id/eprint/43613/ A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain Ali, Md. Eaqub Asing, Abd Hamid, Sharifah Bee Razzak, Md. Abdur Abd Rashid, Nur Raifana Al Amin, Md. Mustafa, Shuhaimi Malayan box turtle (Cuora amboinensis) has been a wildlife-protected vulnerable turtle species in Malaysia since 2005. However, because of its purported usage in traditional medicine, tonic foods and feeds, clandestine black market trade is rampant. Several polymerase chain reaction (PCR) assays for the taxonomic detection and classification of turtle species have been proposed. These assays are based on long-length target amplicons which are assumed to break down under compromised states and, hence, might not be suitable for the forensic tracing and tracking of turtle trafficking. For the first time this paper develops a very short-amplicon-length PCR assay (120 bp) for the detection of Malayan box turtle meat in raw, processed and mixed matrices, and experimental evidence is produced that such an assay is not only more stable and reliable but also more sensitive than those previously published. We checked the assay specificity against 20 different species and no cross-species detection was observed. The possibility of any false-negative detection was eliminated by a universal endogenous control for eukaryotes. The assay detection limit was 0.0001 ng of box turtle DNA from pure meat and 0.01% turtle meat in binary and ternary admixtures and commercial meatballs. Superior target stability and sensitivity under extreme treatments of boiling, autoclaving and microwave cooking suggested that this newly developed assay would be suitable for any forensic and/or archaeological identification of Malayan box turtle species, even in severely degraded specimens. Further, in silico studies indicated that the assay has the potential to be used as a universal probe for the detection of nine Cuora species, all of which are critically endangered. Taylor & Francis Group 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43613/1/A%20suitable%20method%20to%20detect%20potential%20fraud%20of%20bringing%20Malayan%20box%20turtle%20%28Cuora%20amboinensis%29%20meat%20into%20the%20food%20chain.pdf Ali, Md. Eaqub and Asing, and Abd Hamid, Sharifah Bee and Razzak, Md. Abdur and Abd Rashid, Nur Raifana and Al Amin, Md. and Mustafa, Shuhaimi (2015) A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment, 32 (8). pp. 1223-1233. ISSN 1944-0049; ESSN: 1944-0057 http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1058535?journalCode=tfac20 10.1080/19440049.2015.1058535
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Malayan box turtle (Cuora amboinensis) has been a wildlife-protected vulnerable turtle species in Malaysia since 2005. However, because of its purported usage in traditional medicine, tonic foods and feeds, clandestine black market trade is rampant. Several polymerase chain reaction (PCR) assays for the taxonomic detection and classification of turtle species have been proposed. These assays are based on long-length target amplicons which are assumed to break down under compromised states and, hence, might not be suitable for the forensic tracing and tracking of turtle trafficking. For the first time this paper develops a very short-amplicon-length PCR assay (120 bp) for the detection of Malayan box turtle meat in raw, processed and mixed matrices, and experimental evidence is produced that such an assay is not only more stable and reliable but also more sensitive than those previously published. We checked the assay specificity against 20 different species and no cross-species detection was observed. The possibility of any false-negative detection was eliminated by a universal endogenous control for eukaryotes. The assay detection limit was 0.0001 ng of box turtle DNA from pure meat and 0.01% turtle meat in binary and ternary admixtures and commercial meatballs. Superior target stability and sensitivity under extreme treatments of boiling, autoclaving and microwave cooking suggested that this newly developed assay would be suitable for any forensic and/or archaeological identification of Malayan box turtle species, even in severely degraded specimens. Further, in silico studies indicated that the assay has the potential to be used as a universal probe for the detection of nine Cuora species, all of which are critically endangered.
format Article
author Ali, Md. Eaqub
Asing,
Abd Hamid, Sharifah Bee
Razzak, Md. Abdur
Abd Rashid, Nur Raifana
Al Amin, Md.
Mustafa, Shuhaimi
spellingShingle Ali, Md. Eaqub
Asing,
Abd Hamid, Sharifah Bee
Razzak, Md. Abdur
Abd Rashid, Nur Raifana
Al Amin, Md.
Mustafa, Shuhaimi
A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain
author_facet Ali, Md. Eaqub
Asing,
Abd Hamid, Sharifah Bee
Razzak, Md. Abdur
Abd Rashid, Nur Raifana
Al Amin, Md.
Mustafa, Shuhaimi
author_sort Ali, Md. Eaqub
title A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain
title_short A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain
title_full A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain
title_fullStr A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain
title_full_unstemmed A Suitable method to detect potential fraud of bringing Malayan box turtle (Cuora amboinensis) meat into the food chain
title_sort suitable method to detect potential fraud of bringing malayan box turtle (cuora amboinensis) meat into the food chain
publisher Taylor & Francis Group
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/43613/1/A%20suitable%20method%20to%20detect%20potential%20fraud%20of%20bringing%20Malayan%20box%20turtle%20%28Cuora%20amboinensis%29%20meat%20into%20the%20food%20chain.pdf
http://psasir.upm.edu.my/id/eprint/43613/
http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1058535?journalCode=tfac20
_version_ 1643833617421434880
score 13.211869

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