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A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population

The aim of the present study was to assess and compare the antimicrobial susceptibility pattern against a panel of antibiotics and molecular and methicillin resistance-associated genotypes of 120 carriage S. aureus isolates previously isolated from a student population at two isolation events within...

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Main Authors: Mat Azis, Norhidayah, Pung, Hui Ping, Abdul Rachman, Abdul Rahim, Amin Nordin, Syafinaz, Sarchio, Seri Narti Edayu, Suhaili, Zarizal, Mohd Desa, Mohd Nasir
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/43544/1/43544.pdf
http://psasir.upm.edu.my/id/eprint/43544/
http://www.sciencedirect.com/science/article/pii/S1876034116300120
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spelling my.upm.eprints.435442017-05-23T02:17:16Z http://psasir.upm.edu.my/id/eprint/43544/ A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population Mat Azis, Norhidayah Pung, Hui Ping Abdul Rachman, Abdul Rahim Amin Nordin, Syafinaz Sarchio, Seri Narti Edayu Suhaili, Zarizal Mohd Desa, Mohd Nasir The aim of the present study was to assess and compare the antimicrobial susceptibility pattern against a panel of antibiotics and molecular and methicillin resistance-associated genotypes of 120 carriage S. aureus isolates previously isolated from a student population at two isolation events within a one-month interval. The antibiotic susceptibility of isolates was determined using the Kirby-Bauer disc-diffusion method (cefoxitin by Etest). The MRSA was screened using polymerase chain reaction for the presence of the mecA gene. The mecA-positive isolates were subjected to staphylococcal cassette chromosome (SCC) mec typing, multilocus sequence typing (MLST) and eBURST analysis. All isolates were characterized for the presence of the Panton–Valentine leukocidin (PVL) gene, an enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) pattern and the spa type. For the two occasions where S. aureus was isolated, the highest frequency of resistance was observed for penicillin (70% and 65%, respectively), with a lower rate against erythromycin and tetracycline (<12%). All isolates were susceptible to ciprofloxacin and gentamycin. As for methicillin resistance, eight isolates had minimum inhibitory concentrations (MIC) of resistant categories, but 10 isolates (8.33%) were positive for the mecA gene. The mecA-positive isolates belonged to SCCmec types I (n = 9) and V (n = 1). MLST was resolved for only three MRSAs, ST508 (n = 1), ST88 (n = 1) and ST96 (n = 1). The results of the eBURST analysis showed that the MRSA isolates analyzed in the present study were potentially related to MRSA identified in other countries. Approximately half of the persistent S. aureus carriers harbored S. aureus of a similar spa type in the respective individuals during both isolation events. A persistent antimicrobial pattern and limited distinct MRSAs were observed over the short study period. The latter frequently exhibited SCCmec type I, commonly associated with hospital-acquired (HA) characteristics, but further delineation is needed to justify the origins of these bacteria. Elsevier 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43544/1/43544.pdf Mat Azis, Norhidayah and Pung, Hui Ping and Abdul Rachman, Abdul Rahim and Amin Nordin, Syafinaz and Sarchio, Seri Narti Edayu and Suhaili, Zarizal and Mohd Desa, Mohd Nasir (2017) A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population. Journal of Infection and Public Health, 10 (2). pp. 156-164. ISSN 1876-0341; ESSN: 1876-035X http://www.sciencedirect.com/science/article/pii/S1876034116300120 10.1016/j.jiph.2016.02.013
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aim of the present study was to assess and compare the antimicrobial susceptibility pattern against a panel of antibiotics and molecular and methicillin resistance-associated genotypes of 120 carriage S. aureus isolates previously isolated from a student population at two isolation events within a one-month interval. The antibiotic susceptibility of isolates was determined using the Kirby-Bauer disc-diffusion method (cefoxitin by Etest). The MRSA was screened using polymerase chain reaction for the presence of the mecA gene. The mecA-positive isolates were subjected to staphylococcal cassette chromosome (SCC) mec typing, multilocus sequence typing (MLST) and eBURST analysis. All isolates were characterized for the presence of the Panton–Valentine leukocidin (PVL) gene, an enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) pattern and the spa type. For the two occasions where S. aureus was isolated, the highest frequency of resistance was observed for penicillin (70% and 65%, respectively), with a lower rate against erythromycin and tetracycline (<12%). All isolates were susceptible to ciprofloxacin and gentamycin. As for methicillin resistance, eight isolates had minimum inhibitory concentrations (MIC) of resistant categories, but 10 isolates (8.33%) were positive for the mecA gene. The mecA-positive isolates belonged to SCCmec types I (n = 9) and V (n = 1). MLST was resolved for only three MRSAs, ST508 (n = 1), ST88 (n = 1) and ST96 (n = 1). The results of the eBURST analysis showed that the MRSA isolates analyzed in the present study were potentially related to MRSA identified in other countries. Approximately half of the persistent S. aureus carriers harbored S. aureus of a similar spa type in the respective individuals during both isolation events. A persistent antimicrobial pattern and limited distinct MRSAs were observed over the short study period. The latter frequently exhibited SCCmec type I, commonly associated with hospital-acquired (HA) characteristics, but further delineation is needed to justify the origins of these bacteria.
format Article
author Mat Azis, Norhidayah
Pung, Hui Ping
Abdul Rachman, Abdul Rahim
Amin Nordin, Syafinaz
Sarchio, Seri Narti Edayu
Suhaili, Zarizal
Mohd Desa, Mohd Nasir
spellingShingle Mat Azis, Norhidayah
Pung, Hui Ping
Abdul Rachman, Abdul Rahim
Amin Nordin, Syafinaz
Sarchio, Seri Narti Edayu
Suhaili, Zarizal
Mohd Desa, Mohd Nasir
A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population
author_facet Mat Azis, Norhidayah
Pung, Hui Ping
Abdul Rachman, Abdul Rahim
Amin Nordin, Syafinaz
Sarchio, Seri Narti Edayu
Suhaili, Zarizal
Mohd Desa, Mohd Nasir
author_sort Mat Azis, Norhidayah
title A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population
title_short A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population
title_full A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population
title_fullStr A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population
title_full_unstemmed A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population
title_sort persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term staphylococcus aureus carriage isolated from a student population
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/43544/1/43544.pdf
http://psasir.upm.edu.my/id/eprint/43544/
http://www.sciencedirect.com/science/article/pii/S1876034116300120
_version_ 1643833597532045312
score 13.211869

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