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Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast

Kitchen wastes containing high amounts of carbohydrates have potential as low-cost substrates for fermentable sugar production. In this study, enzymatic saccharification of kitchen waste was carried out. Response surface methodology (RSM) was applied to optimize the enzymatic saccharification condit...

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Main Authors: Hafid, Halimatun Saadiah, Abdul Rahman, Nor'aini, Md. Shah, Umi Kalsom, Samsu Baharuddin, Azhari, Zakaria, Rabitah
Format: Article
Language:English
Published: Taylor & Francis 2016
Online Access:http://psasir.upm.edu.my/id/eprint/43070/1/Direct%20utilization%20of%20kitchen%20waste%20for%20bioethanol%20production%20by%20separate%20hydrolysis%20and%20fermentation%20%28SHF%29%20using%20locally%20isolated%20yeast.pdf
http://psasir.upm.edu.my/id/eprint/43070/
http://www.tandfonline.com/doi/abs/10.1080/15435075.2014.940958
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spelling my.upm.eprints.430702016-05-18T01:12:11Z http://psasir.upm.edu.my/id/eprint/43070/ Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast Hafid, Halimatun Saadiah Abdul Rahman, Nor'aini Md. Shah, Umi Kalsom Samsu Baharuddin, Azhari Zakaria, Rabitah Kitchen wastes containing high amounts of carbohydrates have potential as low-cost substrates for fermentable sugar production. In this study, enzymatic saccharification of kitchen waste was carried out. Response surface methodology (RSM) was applied to optimize the enzymatic saccharification conditions of kitchen waste. This paper presents analysis of RSM in a predictive model of the combined effects of independent variables (pH, temperature, glucoamylase activity, kitchen waste loading, and hydrolysis time) as the most significant parameters for fermentable sugar production and degree of saccharification. A 100 mL of kitchen waste was hydrolyzed in 250 mL of shake flasks. Quadratic RSM predicted maximum fermentable sugar production of 62.79 g/L and degree of saccharification (59.90%) at the following optimal conditions: pH 5, temperature 60°C, glucoamylase activity of 85 U/mL, and utilized 60 g/L of kitchen waste as a substrate at 10 h hydrolysis time. The verification experiments successfully produced 62.71 ± 0.7 g/L of fermentable sugar with 54.93 ± 0.4% degree of saccharification within 10 h of incubation, indicating that the developed model was successfully used to predict fermentable sugar production at more than 90% accuracy. The sugars produced after hydrolysis of kitchen waste were mainly attributed to monosaccharide: glucose (80%) and fructose (20%). The fermentable sugars obtained were subsequently used as carbon source for bioethanol production by locally isolated yeasts: Saccharomyces cerevisiae, Candida parasilosis, and Lanchancea fermentati. The yeasts were successfully consumed as sugars hydrolysate, and produced the highest ethanol yield ranging from 0.45 to 0.5 g/g and productivity between 0.44 g L–1 h–1 and 0.47 g L–1 h–1 after 24-h incubation, which was equivalent to 82.06–98.19% of conversion based on theoretical yield. Taylor & Francis 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43070/1/Direct%20utilization%20of%20kitchen%20waste%20for%20bioethanol%20production%20by%20separate%20hydrolysis%20and%20fermentation%20%28SHF%29%20using%20locally%20isolated%20yeast.pdf Hafid, Halimatun Saadiah and Abdul Rahman, Nor'aini and Md. Shah, Umi Kalsom and Samsu Baharuddin, Azhari and Zakaria, Rabitah (2016) Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast. International Journal of Green Energy, 13 (3). pp. 248-259. ISSN 1543-5075; ESSN: 1543-5083 http://www.tandfonline.com/doi/abs/10.1080/15435075.2014.940958 10.1080/15435075.2014.940958
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Kitchen wastes containing high amounts of carbohydrates have potential as low-cost substrates for fermentable sugar production. In this study, enzymatic saccharification of kitchen waste was carried out. Response surface methodology (RSM) was applied to optimize the enzymatic saccharification conditions of kitchen waste. This paper presents analysis of RSM in a predictive model of the combined effects of independent variables (pH, temperature, glucoamylase activity, kitchen waste loading, and hydrolysis time) as the most significant parameters for fermentable sugar production and degree of saccharification. A 100 mL of kitchen waste was hydrolyzed in 250 mL of shake flasks. Quadratic RSM predicted maximum fermentable sugar production of 62.79 g/L and degree of saccharification (59.90%) at the following optimal conditions: pH 5, temperature 60°C, glucoamylase activity of 85 U/mL, and utilized 60 g/L of kitchen waste as a substrate at 10 h hydrolysis time. The verification experiments successfully produced 62.71 ± 0.7 g/L of fermentable sugar with 54.93 ± 0.4% degree of saccharification within 10 h of incubation, indicating that the developed model was successfully used to predict fermentable sugar production at more than 90% accuracy. The sugars produced after hydrolysis of kitchen waste were mainly attributed to monosaccharide: glucose (80%) and fructose (20%). The fermentable sugars obtained were subsequently used as carbon source for bioethanol production by locally isolated yeasts: Saccharomyces cerevisiae, Candida parasilosis, and Lanchancea fermentati. The yeasts were successfully consumed as sugars hydrolysate, and produced the highest ethanol yield ranging from 0.45 to 0.5 g/g and productivity between 0.44 g L–1 h–1 and 0.47 g L–1 h–1 after 24-h incubation, which was equivalent to 82.06–98.19% of conversion based on theoretical yield.
format Article
author Hafid, Halimatun Saadiah
Abdul Rahman, Nor'aini
Md. Shah, Umi Kalsom
Samsu Baharuddin, Azhari
Zakaria, Rabitah
spellingShingle Hafid, Halimatun Saadiah
Abdul Rahman, Nor'aini
Md. Shah, Umi Kalsom
Samsu Baharuddin, Azhari
Zakaria, Rabitah
Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast
author_facet Hafid, Halimatun Saadiah
Abdul Rahman, Nor'aini
Md. Shah, Umi Kalsom
Samsu Baharuddin, Azhari
Zakaria, Rabitah
author_sort Hafid, Halimatun Saadiah
title Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast
title_short Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast
title_full Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast
title_fullStr Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast
title_full_unstemmed Direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (SHF) using locally isolated yeast
title_sort direct utilization of kitchen waste for bioethanol production by separate hydrolysis and fermentation (shf) using locally isolated yeast
publisher Taylor & Francis
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/43070/1/Direct%20utilization%20of%20kitchen%20waste%20for%20bioethanol%20production%20by%20separate%20hydrolysis%20and%20fermentation%20%28SHF%29%20using%20locally%20isolated%20yeast.pdf
http://psasir.upm.edu.my/id/eprint/43070/
http://www.tandfonline.com/doi/abs/10.1080/15435075.2014.940958
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score 13.211869

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