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Production of citric acid from cocoa juice waste

A study was conducted for production of citric acid using cocoa juice as a substrate. Two-staged fermentation protocol was carried out employing ten different microorganisms in shaken flasks at 200 rpm and room temperature. Five percent methanol was added to the screened cultures on the third day of...

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Main Authors: El-Sharkawy, Saleh Hassan, Abdul Karim, M. I.
Format: Article
Language:English
Published: Societa Editoriale Farmaceutica 1996
Online Access:http://psasir.upm.edu.my/id/eprint/42254/1/Production%20of%20citric%20acid%20from%20cocoa%20juice%20waste.pdf
http://psasir.upm.edu.my/id/eprint/42254/
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spelling my.upm.eprints.422542016-04-26T01:21:28Z http://psasir.upm.edu.my/id/eprint/42254/ Production of citric acid from cocoa juice waste El-Sharkawy, Saleh Hassan Abdul Karim, M. I. A study was conducted for production of citric acid using cocoa juice as a substrate. Two-staged fermentation protocol was carried out employing ten different microorganisms in shaken flasks at 200 rpm and room temperature. Five percent methanol was added to the screened cultures on the third day of fermentation to stimulate citric acid production. Of the ten screened microorganisms only Candida lipolytica ATCC 8661 and Aspergillus niger ATCC 10581 showed a pronounced production of citric acid. The fermentation was optimized for the two selected microorganisms to maximize citric acid yield. Maximum yield of citric acid (38.45 mg/ml) was obtained on using C. lipolytica ATCC 8661 growing at room temperature in 25% cocoa juice, 0.5% peptone and pH 6.5 for 9 days. However, A. niger ATCC 10581 produced 45.00 mg/ml at 30°C in 50°/O cocoa juice media supplemented with 0.5% peptone and pH 6.5 for 15 days incubation. Samples were withdrawn at intervals and the citric acid contents were analyzed using HPLC. Optimization of citric acid production using C. lipolytica and A. niger produced 51.33 and 56.21 mg/ml, respectively. Societa Editoriale Farmaceutica 1996 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/42254/1/Production%20of%20citric%20acid%20from%20cocoa%20juice%20waste.pdf El-Sharkawy, Saleh Hassan and Abdul Karim, M. I. (1996) Production of citric acid from cocoa juice waste. Bollettino Chimico Farmaceutico, 135 (3). pp. 176-179. ISSN 0006-6648
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A study was conducted for production of citric acid using cocoa juice as a substrate. Two-staged fermentation protocol was carried out employing ten different microorganisms in shaken flasks at 200 rpm and room temperature. Five percent methanol was added to the screened cultures on the third day of fermentation to stimulate citric acid production. Of the ten screened microorganisms only Candida lipolytica ATCC 8661 and Aspergillus niger ATCC 10581 showed a pronounced production of citric acid. The fermentation was optimized for the two selected microorganisms to maximize citric acid yield. Maximum yield of citric acid (38.45 mg/ml) was obtained on using C. lipolytica ATCC 8661 growing at room temperature in 25% cocoa juice, 0.5% peptone and pH 6.5 for 9 days. However, A. niger ATCC 10581 produced 45.00 mg/ml at 30°C in 50°/O cocoa juice media supplemented with 0.5% peptone and pH 6.5 for 15 days incubation. Samples were withdrawn at intervals and the citric acid contents were analyzed using HPLC. Optimization of citric acid production using C. lipolytica and A. niger produced 51.33 and 56.21 mg/ml, respectively.
format Article
author El-Sharkawy, Saleh Hassan
Abdul Karim, M. I.
spellingShingle El-Sharkawy, Saleh Hassan
Abdul Karim, M. I.
Production of citric acid from cocoa juice waste
author_facet El-Sharkawy, Saleh Hassan
Abdul Karim, M. I.
author_sort El-Sharkawy, Saleh Hassan
title Production of citric acid from cocoa juice waste
title_short Production of citric acid from cocoa juice waste
title_full Production of citric acid from cocoa juice waste
title_fullStr Production of citric acid from cocoa juice waste
title_full_unstemmed Production of citric acid from cocoa juice waste
title_sort production of citric acid from cocoa juice waste
publisher Societa Editoriale Farmaceutica
publishDate 1996
url http://psasir.upm.edu.my/id/eprint/42254/1/Production%20of%20citric%20acid%20from%20cocoa%20juice%20waste.pdf
http://psasir.upm.edu.my/id/eprint/42254/
_version_ 1643833231568535552
score 13.211869

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