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Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel

Lansium domesticum (langsat) is a popular tropical fruit bearing trees across the Southeast Asia region. It is cultivated mainly for its fruit, while the peel of langsat as wastes of fruit consumption has been traditionally used as medicine. The aim of this study was to evaluate the total phenolic c...

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Main Authors: Kee, M. E., Khoo, Hock Eng, Sia , Chiaw Mei, Yim, Hip Seng
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2015
Online Access:http://psasir.upm.edu.my/id/eprint/41825/1/Fractionation%20of%20potent%20antioxidative%20components%20from%20langsat%20%28Lansium%20domesticum%29%20peel.pdf
http://psasir.upm.edu.my/id/eprint/41825/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2038%20(1)%20Feb.%202015/09%20Page%20103-112%20(JTAS%200597-2014).pdf
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spelling my.upm.eprints.418252016-02-10T09:05:10Z http://psasir.upm.edu.my/id/eprint/41825/ Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel Kee, M. E. Khoo, Hock Eng Sia , Chiaw Mei Yim, Hip Seng Lansium domesticum (langsat) is a popular tropical fruit bearing trees across the Southeast Asia region. It is cultivated mainly for its fruit, while the peel of langsat as wastes of fruit consumption has been traditionally used as medicine. The aim of this study was to evaluate the total phenolic content (TPC) and antioxidant activity of different fractions of ethanolic extract of langsat peel. The extract was fractionated using open column chromatography and the antioxidant components were observed based on high-performance thin layer chromatography (HPTLC). TPC of the extract and extract fractions (FI˗FIII) was estimated using Folin-Ciocalteu reagent assay, while their antioxidant activity was evaluated using DPPH radical scavenging assay. Highest TPC was estimated in the ethanolic extract of langsat peel compared to its extract fractions. No significant difference was found for TPC among the extract fractions. The extract also had the highest DPPH radical scavenging activity, followed by FIII and FII, but no radical scavenging activity was observed in FI. The result obtained from HPTLC also showed that no band of antioxidative compounds was observed under UV or white light. Based on the antioxidative components found in the peel extract of langsat, the fruit peel is a potential source of antioxidants for development of nutraceutical. Fractionation of the extract is not an ideal technique for producing nutraceutical. Universiti Putra Malaysia Press 2015-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41825/1/Fractionation%20of%20potent%20antioxidative%20components%20from%20langsat%20%28Lansium%20domesticum%29%20peel.pdf Kee, M. E. and Khoo, Hock Eng and Sia , Chiaw Mei and Yim, Hip Seng (2015) Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel. Pertanika Journal of Tropical Agricultural Science, 38 (1). pp. 103-112. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2038%20(1)%20Feb.%202015/09%20Page%20103-112%20(JTAS%200597-2014).pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Lansium domesticum (langsat) is a popular tropical fruit bearing trees across the Southeast Asia region. It is cultivated mainly for its fruit, while the peel of langsat as wastes of fruit consumption has been traditionally used as medicine. The aim of this study was to evaluate the total phenolic content (TPC) and antioxidant activity of different fractions of ethanolic extract of langsat peel. The extract was fractionated using open column chromatography and the antioxidant components were observed based on high-performance thin layer chromatography (HPTLC). TPC of the extract and extract fractions (FI˗FIII) was estimated using Folin-Ciocalteu reagent assay, while their antioxidant activity was evaluated using DPPH radical scavenging assay. Highest TPC was estimated in the ethanolic extract of langsat peel compared to its extract fractions. No significant difference was found for TPC among the extract fractions. The extract also had the highest DPPH radical scavenging activity, followed by FIII and FII, but no radical scavenging activity was observed in FI. The result obtained from HPTLC also showed that no band of antioxidative compounds was observed under UV or white light. Based on the antioxidative components found in the peel extract of langsat, the fruit peel is a potential source of antioxidants for development of nutraceutical. Fractionation of the extract is not an ideal technique for producing nutraceutical.
format Article
author Kee, M. E.
Khoo, Hock Eng
Sia , Chiaw Mei
Yim, Hip Seng
spellingShingle Kee, M. E.
Khoo, Hock Eng
Sia , Chiaw Mei
Yim, Hip Seng
Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel
author_facet Kee, M. E.
Khoo, Hock Eng
Sia , Chiaw Mei
Yim, Hip Seng
author_sort Kee, M. E.
title Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel
title_short Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel
title_full Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel
title_fullStr Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel
title_full_unstemmed Fractionation of potent antioxidative components from langsat (Lansium domesticum) peel
title_sort fractionation of potent antioxidative components from langsat (lansium domesticum) peel
publisher Universiti Putra Malaysia Press
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/41825/1/Fractionation%20of%20potent%20antioxidative%20components%20from%20langsat%20%28Lansium%20domesticum%29%20peel.pdf
http://psasir.upm.edu.my/id/eprint/41825/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2038%20(1)%20Feb.%202015/09%20Page%20103-112%20(JTAS%200597-2014).pdf
_version_ 1643833110480027648
score 13.211869

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