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Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method

The present work demonstrates and discusses the encapsulation of a model drug using a supercritical anti-solvent method (SAS). In this study, paracetamol was chosen as a model drug and was encapsulated in the poly L lactic acid (L-PLA), a semi crystalline polymer, under different process parameters...

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Main Author: Lankan, Mahshid
Format: Thesis
Language:English
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/41794/7/FK%202011%2019R.pdf
http://psasir.upm.edu.my/id/eprint/41794/
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spelling my.upm.eprints.417942016-06-30T03:18:42Z http://psasir.upm.edu.my/id/eprint/41794/ Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method Lankan, Mahshid The present work demonstrates and discusses the encapsulation of a model drug using a supercritical anti-solvent method (SAS). In this study, paracetamol was chosen as a model drug and was encapsulated in the poly L lactic acid (L-PLA), a semi crystalline polymer, under different process parameters namely pressure, temperature, and polymer concentration. The produced nanoparticles were completely spherical with very small size (300 nm) and narrow distribution. Also, the optimum process parameters to produce the smaller particle size were studied by response surface methodology (RSM) statistical software. The nano encapsulated paracetamol release profile demonstrated a long and sustained release of drug in which 70% of paracetamol release was recorded in 4 weeks. The drug release profile of paracetamol inside the PBS buffer solution was fitted with Korsmeyer Peppas kinetic model based on the R2 value equal to 0.987. The first burst happened after 1 week. The size and morphology of the encapsulated nano-particles were characterized by scanning electron microscopy. Transmission electron microscopy (TEM) revealed the internal structure of nano-encapsulated paracetamol and verified the full coating of the drug particle with biodegradable polymer. The results demonstrated that increasing the pressure and decreasing the temperature reduce the mean particle size. These results also showed that the particle size is influenced by the degree of super-saturation and initial polymer concentration, simultaneously. Thus, it is crucial to balance the rate of crystallization and the rate of growth. The optimum process parameters to produce minimum mean particle size (301nm) were obtained at 120 bar, 30ºC, and 16 ppm polymer concentration based on both SEM images and statistical analysis. The thermal characteristics of nano-particles were investigated via differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA). The TGA characteristics of nanoparticles were similar to the TGA characteristic of pure polymer due to the higher ratio of polymer in solute. Conversely, the DSC characteristics of nano-particles were similar to paracetamol characteristic due to the higher heat capacity of paracetamol. Based on the DSC thermograms, the intensity of the endothermal melting peak of pure paracetamol was considerably reduced during SAS process due to the changing of nano-particles structure with respect to the pure L-PLA. This issue was also confirmed by the X-ray diffraction pattern as well. All peaks related to both polymer and drug crystallographs were exhibited in the nano-particles crystallography. Fourier transform infrared spectroscopy (FTIR) investigated the chemical composition of nano-encapsulated paracetamol inside L-PLA. The positions of spectra peaks in FTIR for the encapsulated paracetamol were similar to the absorption peaks of pure polymer due to the high ratio of polymer over drug. The stability of nanoparticles demonstrated by high negative electric charge (-33 ± 3 mV) on the surface of nano particles was confirmed by means of zeta potential characteristic. This high negative surface charge may be due to the presence of carboxyl end groups of L- PLA chain. 2011-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41794/7/FK%202011%2019R.pdf Lankan, Mahshid (2011) Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method. Masters thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The present work demonstrates and discusses the encapsulation of a model drug using a supercritical anti-solvent method (SAS). In this study, paracetamol was chosen as a model drug and was encapsulated in the poly L lactic acid (L-PLA), a semi crystalline polymer, under different process parameters namely pressure, temperature, and polymer concentration. The produced nanoparticles were completely spherical with very small size (300 nm) and narrow distribution. Also, the optimum process parameters to produce the smaller particle size were studied by response surface methodology (RSM) statistical software. The nano encapsulated paracetamol release profile demonstrated a long and sustained release of drug in which 70% of paracetamol release was recorded in 4 weeks. The drug release profile of paracetamol inside the PBS buffer solution was fitted with Korsmeyer Peppas kinetic model based on the R2 value equal to 0.987. The first burst happened after 1 week. The size and morphology of the encapsulated nano-particles were characterized by scanning electron microscopy. Transmission electron microscopy (TEM) revealed the internal structure of nano-encapsulated paracetamol and verified the full coating of the drug particle with biodegradable polymer. The results demonstrated that increasing the pressure and decreasing the temperature reduce the mean particle size. These results also showed that the particle size is influenced by the degree of super-saturation and initial polymer concentration, simultaneously. Thus, it is crucial to balance the rate of crystallization and the rate of growth. The optimum process parameters to produce minimum mean particle size (301nm) were obtained at 120 bar, 30ºC, and 16 ppm polymer concentration based on both SEM images and statistical analysis. The thermal characteristics of nano-particles were investigated via differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA). The TGA characteristics of nanoparticles were similar to the TGA characteristic of pure polymer due to the higher ratio of polymer in solute. Conversely, the DSC characteristics of nano-particles were similar to paracetamol characteristic due to the higher heat capacity of paracetamol. Based on the DSC thermograms, the intensity of the endothermal melting peak of pure paracetamol was considerably reduced during SAS process due to the changing of nano-particles structure with respect to the pure L-PLA. This issue was also confirmed by the X-ray diffraction pattern as well. All peaks related to both polymer and drug crystallographs were exhibited in the nano-particles crystallography. Fourier transform infrared spectroscopy (FTIR) investigated the chemical composition of nano-encapsulated paracetamol inside L-PLA. The positions of spectra peaks in FTIR for the encapsulated paracetamol were similar to the absorption peaks of pure polymer due to the high ratio of polymer over drug. The stability of nanoparticles demonstrated by high negative electric charge (-33 ± 3 mV) on the surface of nano particles was confirmed by means of zeta potential characteristic. This high negative surface charge may be due to the presence of carboxyl end groups of L- PLA chain.
format Thesis
author Lankan, Mahshid
spellingShingle Lankan, Mahshid
Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method
author_facet Lankan, Mahshid
author_sort Lankan, Mahshid
title Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method
title_short Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method
title_full Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method
title_fullStr Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method
title_full_unstemmed Nano-encapsulation of paracetamol in L-poly lactic acid using supercritical anti-solvent method
title_sort nano-encapsulation of paracetamol in l-poly lactic acid using supercritical anti-solvent method
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/41794/7/FK%202011%2019R.pdf
http://psasir.upm.edu.my/id/eprint/41794/
_version_ 1643833101619560448
score 13.211869

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