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Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples

Routine diagnosis of intestinal microsporidiosis in clinical diagnostic laboratories relies mostly on detection of microsporidial spores via special staining and microscopic techniques. This paper describes the comparative evaluation of Calcofluor White M2R method, with modified Gram-chromotrope Kin...

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Main Authors: Ithnin, Nur Raihana, Osman, Malina, M. S., Fatmah, M., Norhayati, Abdul Rahman, Eni Juraida, Hamat, Rukman Awang
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2013
Online Access:http://psasir.upm.edu.my/id/eprint/41366/1/Comparison%20of%20calcofluor%20white%20M2R%20fluorescence.pdf
http://psasir.upm.edu.my/id/eprint/41366/
http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2013V09N2_OP08.pdf
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spelling my.upm.eprints.413662015-12-18T01:02:17Z http://psasir.upm.edu.my/id/eprint/41366/ Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples Ithnin, Nur Raihana Osman, Malina M. S., Fatmah M., Norhayati Abdul Rahman, Eni Juraida Hamat, Rukman Awang Routine diagnosis of intestinal microsporidiosis in clinical diagnostic laboratories relies mostly on detection of microsporidial spores via special staining and microscopic techniques. This paper describes the comparative evaluation of Calcofluor White M2R method, with modified Gram-chromotrope Kinyoun method as the reference standard. One hundred and six stool samples were examined for the presence of microsporidial spores. Sensitivity, specificity, positive and negative predictive values of the Calcofluor White M2R method compared to the reference technique were 95.2%, 4.3%, 78.2% and 20.0%, respectively. The positive predictive value (PPV) was 78.2% and the negative predictive value (NPV) was 20.0%. Despite low specificity of the CFW method due to its ability to stain chitinous wall of microorganisms, the presence of distinct deep-blue horizontal or equitorial stripes in microsporidial spores in modified Gram-chromotrope Kinyoun would likely reduce the false positive results obtained in the Calcofluor White M2R. Hence, the simultaneous use of these two methods would give better performance and accuracy for the detection of microsporidial spores in patients with intestinal microsporidiosis. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2013-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41366/1/Comparison%20of%20calcofluor%20white%20M2R%20fluorescence.pdf Ithnin, Nur Raihana and Osman, Malina and M. S., Fatmah and M., Norhayati and Abdul Rahman, Eni Juraida and Hamat, Rukman Awang (2013) Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples. Malaysian Journal of Medicine and Health Sciences, 9 (2). pp. 63-68. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2013V09N2_OP08.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Routine diagnosis of intestinal microsporidiosis in clinical diagnostic laboratories relies mostly on detection of microsporidial spores via special staining and microscopic techniques. This paper describes the comparative evaluation of Calcofluor White M2R method, with modified Gram-chromotrope Kinyoun method as the reference standard. One hundred and six stool samples were examined for the presence of microsporidial spores. Sensitivity, specificity, positive and negative predictive values of the Calcofluor White M2R method compared to the reference technique were 95.2%, 4.3%, 78.2% and 20.0%, respectively. The positive predictive value (PPV) was 78.2% and the negative predictive value (NPV) was 20.0%. Despite low specificity of the CFW method due to its ability to stain chitinous wall of microorganisms, the presence of distinct deep-blue horizontal or equitorial stripes in microsporidial spores in modified Gram-chromotrope Kinyoun would likely reduce the false positive results obtained in the Calcofluor White M2R. Hence, the simultaneous use of these two methods would give better performance and accuracy for the detection of microsporidial spores in patients with intestinal microsporidiosis.
format Article
author Ithnin, Nur Raihana
Osman, Malina
M. S., Fatmah
M., Norhayati
Abdul Rahman, Eni Juraida
Hamat, Rukman Awang
spellingShingle Ithnin, Nur Raihana
Osman, Malina
M. S., Fatmah
M., Norhayati
Abdul Rahman, Eni Juraida
Hamat, Rukman Awang
Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples
author_facet Ithnin, Nur Raihana
Osman, Malina
M. S., Fatmah
M., Norhayati
Abdul Rahman, Eni Juraida
Hamat, Rukman Awang
author_sort Ithnin, Nur Raihana
title Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples
title_short Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples
title_full Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples
title_fullStr Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples
title_full_unstemmed Comparison of Calcofluor White M2R fluorescence and modified Gram chromotrope Kinyoun staining methods for the detection of microsporidial spores from stool samples
title_sort comparison of calcofluor white m2r fluorescence and modified gram chromotrope kinyoun staining methods for the detection of microsporidial spores from stool samples
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/41366/1/Comparison%20of%20calcofluor%20white%20M2R%20fluorescence.pdf
http://psasir.upm.edu.my/id/eprint/41366/
http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2013V09N2_OP08.pdf
_version_ 1643832976354574336
score 13.211869

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