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Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines

Objective: Previous studies have shown milk to contain cancer inhibitors. In this context, this study was conducted to screen the potential cytotoxic properties of four different types of milk, namely cow's milk, goat's milk, mare's milk and human milk. Methods: In evaluating the cyto...

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Main Authors: Rahmat, Asmah, Rosli, Rozita, Tan, Mui Hoon, Umar-Tsafe, Nasir, Ali, Abdul Manaf, Abu Bakar, Mohd Fadzelly
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2006
Online Access:http://psasir.upm.edu.my/id/eprint/41161/1/Comparative%20evaluation%20of%20cytotoxic%20effects%20of%20milk%20from%20various%20species%20on%20leukemia%20cell%20lines.pdf
http://psasir.upm.edu.my/id/eprint/41161/
http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2006V02N1_OP01.pdf
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spelling my.upm.eprints.411612015-12-03T06:22:24Z http://psasir.upm.edu.my/id/eprint/41161/ Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines Rahmat, Asmah Rosli, Rozita Tan, Mui Hoon Umar-Tsafe, Nasir Ali, Abdul Manaf Abu Bakar, Mohd Fadzelly Objective: Previous studies have shown milk to contain cancer inhibitors. In this context, this study was conducted to screen the potential cytotoxic properties of four different types of milk, namely cow's milk, goat's milk, mare's milk and human milk. Methods: In evaluating the cytotoxic properties of milk, two different human leukemia cell lines namely, Raji and CEM-SS were used. The treated and untreated cells of milk were cultured at 37°C in 5% CO2 for 5 days according to standard guidelines. The CellTiter 96® Aqueous (MTS) assay was carried out on the first, third and fifth days to measure cell viability. The percentage of cell viability was determined by comparing the optical density of the treated cells against the untreated controls. One-way ANOYA at p<O.05 , Duncan's Multiple Range test and Independent T-test were carried out to determine the mean count differences of cell viability between and within cow's milk, goat's milk, mare's milk and human milk. Results: Interestingly, only mare's milk was found to cause statistically significant cytotoxicity on both the Raji (p<O.05) and CEM-SS (p<O.05) cells at 10% dilution. The cells treated with cow's milk, goat's milk and human milk showed no significant difference in cell viability when compared to the untreated controls where cell growth effects were observed instead. In addition, the cells treated with mare's milk underwent morphological changes that were indicative of apoptosis. Conclusion: The findings of this study show that among the four types of milk, mare's milk appears to possess cytotoxic properties against leukemia cell lines. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2006-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41161/1/Comparative%20evaluation%20of%20cytotoxic%20effects%20of%20milk%20from%20various%20species%20on%20leukemia%20cell%20lines.pdf Rahmat, Asmah and Rosli, Rozita and Tan, Mui Hoon and Umar-Tsafe, Nasir and Ali, Abdul Manaf and Abu Bakar, Mohd Fadzelly (2006) Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines. Malaysian Journal of Medicine and Health Sciences, 2 (1). pp. 1-10. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2006V02N1_OP01.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Objective: Previous studies have shown milk to contain cancer inhibitors. In this context, this study was conducted to screen the potential cytotoxic properties of four different types of milk, namely cow's milk, goat's milk, mare's milk and human milk. Methods: In evaluating the cytotoxic properties of milk, two different human leukemia cell lines namely, Raji and CEM-SS were used. The treated and untreated cells of milk were cultured at 37°C in 5% CO2 for 5 days according to standard guidelines. The CellTiter 96® Aqueous (MTS) assay was carried out on the first, third and fifth days to measure cell viability. The percentage of cell viability was determined by comparing the optical density of the treated cells against the untreated controls. One-way ANOYA at p<O.05 , Duncan's Multiple Range test and Independent T-test were carried out to determine the mean count differences of cell viability between and within cow's milk, goat's milk, mare's milk and human milk. Results: Interestingly, only mare's milk was found to cause statistically significant cytotoxicity on both the Raji (p<O.05) and CEM-SS (p<O.05) cells at 10% dilution. The cells treated with cow's milk, goat's milk and human milk showed no significant difference in cell viability when compared to the untreated controls where cell growth effects were observed instead. In addition, the cells treated with mare's milk underwent morphological changes that were indicative of apoptosis. Conclusion: The findings of this study show that among the four types of milk, mare's milk appears to possess cytotoxic properties against leukemia cell lines.
format Article
author Rahmat, Asmah
Rosli, Rozita
Tan, Mui Hoon
Umar-Tsafe, Nasir
Ali, Abdul Manaf
Abu Bakar, Mohd Fadzelly
spellingShingle Rahmat, Asmah
Rosli, Rozita
Tan, Mui Hoon
Umar-Tsafe, Nasir
Ali, Abdul Manaf
Abu Bakar, Mohd Fadzelly
Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines
author_facet Rahmat, Asmah
Rosli, Rozita
Tan, Mui Hoon
Umar-Tsafe, Nasir
Ali, Abdul Manaf
Abu Bakar, Mohd Fadzelly
author_sort Rahmat, Asmah
title Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines
title_short Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines
title_full Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines
title_fullStr Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines
title_full_unstemmed Comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines
title_sort comparative evaluation of cytotoxic effects of milk from various species on leukemia cell lines
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/41161/1/Comparative%20evaluation%20of%20cytotoxic%20effects%20of%20milk%20from%20various%20species%20on%20leukemia%20cell%20lines.pdf
http://psasir.upm.edu.my/id/eprint/41161/
http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2006V02N1_OP01.pdf
_version_ 1643832917839839232
score 13.211869

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