Screening for thalassaemia in pregnant women: a laboratory perspective
Aim: To investigate whether in Malaysia, a mean corpuscular volume (MCV) less than 80 f1 and mean corpuscular haemoglobin (MCH) less than 27 pg will identify carriers in pregnant women with severe forms of thalassaemia, α-thal 1 (α°) and classical β (β°)- thalassaemia. The results from this study wi...
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| التنسيق: | مقال |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2005
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| الوصول للمادة أونلاين: | http://psasir.upm.edu.my/id/eprint/41145/1/Screening%20for%20thalassaemia%20in%20pregnant%20women%20a%20laboratory%20perspective.pdf http://psasir.upm.edu.my/id/eprint/41145/ http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2005V01N2_OP04.pdf |
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my.upm.eprints.411452015-12-03T04:59:55Z http://psasir.upm.edu.my/id/eprint/41145/ Screening for thalassaemia in pregnant women: a laboratory perspective George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini Aim: To investigate whether in Malaysia, a mean corpuscular volume (MCV) less than 80 f1 and mean corpuscular haemoglobin (MCH) less than 27 pg will identify carriers in pregnant women with severe forms of thalassaemia, α-thal 1 (α°) and classical β (β°)- thalassaemia. The results from this study will aid the implementation of a national program to screen for thalassaemia. Methods: For classical β (β°)- thalassaemia, blood samples collected in EDTA from 153 pregnant women were taken for full blood counts and haemoglobin subtyping by automated blood counting and high performance liquid chromatography (HPLC) respectively. For α-thal 1 (α°), the full blood counts were obtained from archives of 30 pregnant women who were genotyped positive for the α-thal 1 (α°) during prenatal diagnosis for Hb Barts hydrops fetalis. The effects of storage on MCV, MCH and Hb A₂ were determined by tests done daily for 3 weeks. Results: By correlating red cell indices with high performance liquid chromatography and genotypes data, we show that mean corpuscular volume (MCV) <80f1 and mean corpuscular haemoglobin <27pg is able to detect all heterozygous carriers with classical β (β°)- thalassaemia. On storage, MCV of heterozygous carriers with classical β (β°)- thalassaemia rose at 1% a day after 24 hours reaching a mean of 80 f1 by day 15. However, the MCH and Hb A₂ were stable for 3 weeks. Conclusion: A mean corpuscular haemoglobin <27pg should be recommended as cut-off values for screening of carries of α-thal 1 (α°) and classical β (β°)- thalassaemia. In blood samples, not processed within a day, MCH with a cut-off 27pg is the recommended choice for screening of carriers. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2005-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41145/1/Screening%20for%20thalassaemia%20in%20pregnant%20women%20a%20laboratory%20perspective.pdf George, Elizabeth and Khoo, S. K. and Abu Bakar, Mokhtar and Umar, Nor Aini (2005) Screening for thalassaemia in pregnant women: a laboratory perspective. Malaysian Journal of Medicine and Health Sciences, 1 (2). pp. 111-117. ISSN 1675-8544 http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2005V01N2_OP04.pdf |
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Universiti Putra Malaysia |
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http://psasir.upm.edu.my/ |
| language |
English |
| description |
Aim: To investigate whether in Malaysia, a mean corpuscular volume (MCV) less than 80 f1 and mean corpuscular haemoglobin (MCH) less than 27 pg will identify carriers in pregnant women with severe forms of thalassaemia, α-thal 1 (α°) and classical β (β°)- thalassaemia. The results from this study will aid the implementation of a national program to screen for thalassaemia. Methods: For classical β (β°)- thalassaemia, blood samples collected in EDTA from 153 pregnant women were taken for full blood counts and haemoglobin subtyping by automated blood counting and high performance liquid chromatography (HPLC) respectively. For α-thal 1 (α°), the full blood counts were obtained from archives of 30 pregnant women who were genotyped positive for the α-thal 1 (α°) during prenatal diagnosis for Hb Barts hydrops fetalis. The effects of storage on MCV, MCH and Hb A₂ were determined by tests done daily for 3 weeks. Results: By correlating red cell indices with high performance liquid chromatography and genotypes data, we show that mean corpuscular volume (MCV) <80f1 and mean corpuscular haemoglobin <27pg is able to detect all heterozygous carriers with classical β (β°)- thalassaemia. On storage, MCV of heterozygous carriers with classical β (β°)- thalassaemia rose at 1% a day after 24 hours reaching a mean of 80 f1 by day 15. However, the MCH and Hb A₂ were stable for 3 weeks. Conclusion: A mean corpuscular haemoglobin <27pg should be recommended as cut-off values for screening of carries of α-thal 1 (α°) and classical β (β°)- thalassaemia. In blood samples, not processed within a day, MCH with a cut-off 27pg is the recommended choice for screening of carriers. |
| format |
Article |
| author |
George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini |
| spellingShingle |
George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini Screening for thalassaemia in pregnant women: a laboratory perspective |
| author_facet |
George, Elizabeth Khoo, S. K. Abu Bakar, Mokhtar Umar, Nor Aini |
| author_sort |
George, Elizabeth |
| title |
Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_short |
Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_full |
Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_fullStr |
Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_full_unstemmed |
Screening for thalassaemia in pregnant women: a laboratory perspective |
| title_sort |
screening for thalassaemia in pregnant women: a laboratory perspective |
| publisher |
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| publishDate |
2005 |
| url |
http://psasir.upm.edu.my/id/eprint/41145/1/Screening%20for%20thalassaemia%20in%20pregnant%20women%20a%20laboratory%20perspective.pdf http://psasir.upm.edu.my/id/eprint/41145/ http://www.medic.upm.edu.my/dokumen/FKUSK1_MJMHS_2005V01N2_OP04.pdf |
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1643832912977592320 |
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13.250246 |