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Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR

The use of component from Ganoderma lucidum as prebiotic source is interesting as the G. lucidum itself was known for more than a decade in the traditional Chinese medicine. In this work, Ganoderma lucidum crude polysaccharides (GLCP) and Polysaccharide-fraction number 2 (PF-2) were used as carbon s...

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Main Authors: Shaari, Yamin, Mustafa, Shuhaimi, Ariff, Arbakariya, Abu Bakar, Fatimah, Abdul Khalil, Khalilah, Othaman, Muhammad Anas, Abd Manap, Mohd Yazid
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2012
Online Access:http://psasir.upm.edu.my/id/eprint/41010/1/Effect%20of%20Ganoderma%20lucidum%20polysaccharides%20on%20the%20growth%20of%20Bifidobacterium%20spp.%20as%20assessed%20using%20real-time%20PCR.pdf
http://psasir.upm.edu.my/id/eprint/41010/
http://www.ifrj.upm.edu.my/19%20%2803%29%202012/%2856%29%20IFRJ%2019%20%2803%29%202012%20Shuhaimi.pdf
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spelling my.upm.eprints.410102015-11-30T04:46:49Z http://psasir.upm.edu.my/id/eprint/41010/ Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR Shaari, Yamin Mustafa, Shuhaimi Ariff, Arbakariya Abu Bakar, Fatimah Abdul Khalil, Khalilah Othaman, Muhammad Anas Abd Manap, Mohd Yazid The use of component from Ganoderma lucidum as prebiotic source is interesting as the G. lucidum itself was known for more than a decade in the traditional Chinese medicine. In this work, Ganoderma lucidum crude polysaccharides (GLCP) and Polysaccharide-fraction number 2 (PF-2) were used as carbon sources in the fermentation with Bifidobacterium sp. The results showed the potential of prebiotic effect of the G. lucidum extract in batch-culture fermentation based on increment in the growth of bacteria used (0.4-1.5 log10CFU/mL) after 18h fermentation. Fermentation was further done using faecal materials as bacterial inocula and bacterial growth changes were examined using real-time PCR. The results showed the ability of GLCP and PF-2 to support the growth of Bifidobacterium genus with 0.3 and 0.7 log10cells/ml increased, respectively. Interestingly, Lactobacillus which is known as beneficial bacterial genus also showed growth increment with 0.7 and 1 log10cells/ml increased. The competition for carbon sources thus inhibits the growth of potentially harmful genus, Salmonella (0.3 and 0.5 log10cells/ml) in comparison to the control. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41010/1/Effect%20of%20Ganoderma%20lucidum%20polysaccharides%20on%20the%20growth%20of%20Bifidobacterium%20spp.%20as%20assessed%20using%20real-time%20PCR.pdf Shaari, Yamin and Mustafa, Shuhaimi and Ariff, Arbakariya and Abu Bakar, Fatimah and Abdul Khalil, Khalilah and Othaman, Muhammad Anas and Abd Manap, Mohd Yazid (2012) Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR. International Food Research Journal, 19 (3). pp. 1199-1205. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/19%20%2803%29%202012/%2856%29%20IFRJ%2019%20%2803%29%202012%20Shuhaimi.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The use of component from Ganoderma lucidum as prebiotic source is interesting as the G. lucidum itself was known for more than a decade in the traditional Chinese medicine. In this work, Ganoderma lucidum crude polysaccharides (GLCP) and Polysaccharide-fraction number 2 (PF-2) were used as carbon sources in the fermentation with Bifidobacterium sp. The results showed the potential of prebiotic effect of the G. lucidum extract in batch-culture fermentation based on increment in the growth of bacteria used (0.4-1.5 log10CFU/mL) after 18h fermentation. Fermentation was further done using faecal materials as bacterial inocula and bacterial growth changes were examined using real-time PCR. The results showed the ability of GLCP and PF-2 to support the growth of Bifidobacterium genus with 0.3 and 0.7 log10cells/ml increased, respectively. Interestingly, Lactobacillus which is known as beneficial bacterial genus also showed growth increment with 0.7 and 1 log10cells/ml increased. The competition for carbon sources thus inhibits the growth of potentially harmful genus, Salmonella (0.3 and 0.5 log10cells/ml) in comparison to the control.
format Article
author Shaari, Yamin
Mustafa, Shuhaimi
Ariff, Arbakariya
Abu Bakar, Fatimah
Abdul Khalil, Khalilah
Othaman, Muhammad Anas
Abd Manap, Mohd Yazid
spellingShingle Shaari, Yamin
Mustafa, Shuhaimi
Ariff, Arbakariya
Abu Bakar, Fatimah
Abdul Khalil, Khalilah
Othaman, Muhammad Anas
Abd Manap, Mohd Yazid
Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR
author_facet Shaari, Yamin
Mustafa, Shuhaimi
Ariff, Arbakariya
Abu Bakar, Fatimah
Abdul Khalil, Khalilah
Othaman, Muhammad Anas
Abd Manap, Mohd Yazid
author_sort Shaari, Yamin
title Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR
title_short Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR
title_full Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR
title_fullStr Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR
title_full_unstemmed Effect of Ganoderma lucidum polysaccharides on the growth of Bifidobacterium spp. as assessed using real-time PCR
title_sort effect of ganoderma lucidum polysaccharides on the growth of bifidobacterium spp. as assessed using real-time pcr
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/41010/1/Effect%20of%20Ganoderma%20lucidum%20polysaccharides%20on%20the%20growth%20of%20Bifidobacterium%20spp.%20as%20assessed%20using%20real-time%20PCR.pdf
http://psasir.upm.edu.my/id/eprint/41010/
http://www.ifrj.upm.edu.my/19%20%2803%29%202012/%2856%29%20IFRJ%2019%20%2803%29%202012%20Shuhaimi.pdf
_version_ 1643832876367609856
score 13.211869

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