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Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)

1. The activities of hexokinase and carnitine palmitoyltransferase (CPT) activities were assayed in flight muscle mitochondria of Valanga nigricornis. 2. Kinetic analysis of the enzyme activities yielded the following information: for hexokinase, its Km (glucose) is 150 μM while its Vmax is 2.5 μmol...

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Main Authors: Lee, C. W., Tan, C. H.
Format: Article
Language:English
English
Published: Pergamon Press 1983
Online Access:http://psasir.upm.edu.my/id/eprint/40470/1/Hexokinase%20and%20carnitine%20palmitoyltransferase%20activities%20in%20flight%20muscles%20of%20grasshopper%20Valanga%20nigricornis%28burm.%29.pdf
http://psasir.upm.edu.my/id/eprint/40470/7/40470.pdf
http://psasir.upm.edu.my/id/eprint/40470/
http://www.sciencedirect.com/science/article/pii/0305049183901463
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spelling my.upm.eprints.404702024-10-15T06:36:05Z http://psasir.upm.edu.my/id/eprint/40470/ Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.) Lee, C. W. Tan, C. H. 1. The activities of hexokinase and carnitine palmitoyltransferase (CPT) activities were assayed in flight muscle mitochondria of Valanga nigricornis. 2. Kinetic analysis of the enzyme activities yielded the following information: for hexokinase, its Km (glucose) is 150 μM while its Vmax is 2.5 μmol/min/mg protein; the corresponding data for CPT are Km (palmitoylCoA) of 39 μM and Vmax of 22.2 nmol/min/mg mitochondrial protein. 3. Under conditions of equivalent rates of ATP production the hexokinase-CPT activity ratio is 64, suggesting that although V. nigricornis is a “mixed” fuel utilizer, it appears that carbohydrate oxidation constitutes the predominant energy source. Pergamon Press 1983 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/40470/1/Hexokinase%20and%20carnitine%20palmitoyltransferase%20activities%20in%20flight%20muscles%20of%20grasshopper%20Valanga%20nigricornis%28burm.%29.pdf text en http://psasir.upm.edu.my/id/eprint/40470/7/40470.pdf Lee, C. W. and Tan, C. H. (1983) Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 74 (4). pp. 791-792. ISSN 0305-0491 http://www.sciencedirect.com/science/article/pii/0305049183901463 10.1016/0305-0491(83)90146-3
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description 1. The activities of hexokinase and carnitine palmitoyltransferase (CPT) activities were assayed in flight muscle mitochondria of Valanga nigricornis. 2. Kinetic analysis of the enzyme activities yielded the following information: for hexokinase, its Km (glucose) is 150 μM while its Vmax is 2.5 μmol/min/mg protein; the corresponding data for CPT are Km (palmitoylCoA) of 39 μM and Vmax of 22.2 nmol/min/mg mitochondrial protein. 3. Under conditions of equivalent rates of ATP production the hexokinase-CPT activity ratio is 64, suggesting that although V. nigricornis is a “mixed” fuel utilizer, it appears that carbohydrate oxidation constitutes the predominant energy source.
format Article
author Lee, C. W.
Tan, C. H.
spellingShingle Lee, C. W.
Tan, C. H.
Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)
author_facet Lee, C. W.
Tan, C. H.
author_sort Lee, C. W.
title Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)
title_short Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)
title_full Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)
title_fullStr Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)
title_full_unstemmed Hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper Valanga nigricornis(burm.)
title_sort hexokinase and carnitine palmitoyltransferase activities in flight muscles of grasshopper valanga nigricornis(burm.)
publisher Pergamon Press
publishDate 1983
url http://psasir.upm.edu.my/id/eprint/40470/1/Hexokinase%20and%20carnitine%20palmitoyltransferase%20activities%20in%20flight%20muscles%20of%20grasshopper%20Valanga%20nigricornis%28burm.%29.pdf
http://psasir.upm.edu.my/id/eprint/40470/7/40470.pdf
http://psasir.upm.edu.my/id/eprint/40470/
http://www.sciencedirect.com/science/article/pii/0305049183901463
_version_ 1814054564967481344
score 13.211869

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