Staff View: In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)

In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)

The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell...

Full description

Saved in:

Bibliographic Details
Main Authors:	Mohd Isa, Noorain, Abdelwahab, Siddig Ibrahim, Mohan, Syam, Abdul, Ahmad Bustaman, Sukari, Mohd Aspollah, Taha, Manal Mohamed Elhassan, Syam, Suvitha, Mohd Fauzi, Putri Narrima, Cheah, Shiau Chuen, Ahmad, Syahida, Mustafa, Mohammad Rais
Format:	Article
Language:	English
Published:	Associacao Brasileira de Divulgacao Cientifica 2012
Online Access:	http://psasir.upm.edu.my/id/eprint/40428/1/In%20vitro%20anti-inflammatory%2C%20cytotoxic%20and%20antioxidant%20activities%20of%20boesenbergin%20A%2C%20a%20chalcone%20isolated%20from%20Boesenbergia%20rotunda%20%28L.%29%20%28fingerroot%29.pdf http://psasir.upm.edu.my/id/eprint/40428/ http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012000600008&lng=en&nrm=iso&tlng=en
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.upm.eprints.40428
record_format	eprints
spelling	my.upm.eprints.404282015-09-18T02:39:20Z http://psasir.upm.edu.my/id/eprint/40428/ In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot) Mohd Isa, Noorain Abdelwahab, Siddig Ibrahim Mohan, Syam Abdul, Ahmad Bustaman Sukari, Mohd Aspollah Taha, Manal Mohamed Elhassan Syam, Suvitha Mohd Fauzi, Putri Narrima Cheah, Shiau Chuen Ahmad, Syahida Mustafa, Mohammad Rais The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 ± 3.15, 10.69 ± 2.64, 20.31 ± 1.34, 94.10 ± 1.19, and 9.324 ± 0.24 µg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 µg/mL) and quercetin (5 µg/mL) were equivalent to a Trolox concentration of 11.91 ± 0.23 and 160.32 ± 2.75 µM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 µg/mL and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 µg/mL. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract. Associacao Brasileira de Divulgacao Cientifica 2012-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40428/1/In%20vitro%20anti-inflammatory%2C%20cytotoxic%20and%20antioxidant%20activities%20of%20boesenbergin%20A%2C%20a%20chalcone%20isolated%20from%20Boesenbergia%20rotunda%20%28L.%29%20%28fingerroot%29.pdf Mohd Isa, Noorain and Abdelwahab, Siddig Ibrahim and Mohan, Syam and Abdul, Ahmad Bustaman and Sukari, Mohd Aspollah and Taha, Manal Mohamed Elhassan and Syam, Suvitha and Mohd Fauzi, Putri Narrima and Cheah, Shiau Chuen and Ahmad, Syahida and Mustafa, Mohammad Rais (2012) In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot). Brazilian Journal of Medical and Biological Research, 45 (6). pp. 524-530. ISSN 0100-879X; ESSN: 1678-4510 http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012000600008&lng=en&nrm=iso&tlng=en 10.1590/S0100-879X2012007500022
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 ± 3.15, 10.69 ± 2.64, 20.31 ± 1.34, 94.10 ± 1.19, and 9.324 ± 0.24 µg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 µg/mL) and quercetin (5 µg/mL) were equivalent to a Trolox concentration of 11.91 ± 0.23 and 160.32 ± 2.75 µM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 µg/mL and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 µg/mL. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract.
format	Article
author	Mohd Isa, Noorain Abdelwahab, Siddig Ibrahim Mohan, Syam Abdul, Ahmad Bustaman Sukari, Mohd Aspollah Taha, Manal Mohamed Elhassan Syam, Suvitha Mohd Fauzi, Putri Narrima Cheah, Shiau Chuen Ahmad, Syahida Mustafa, Mohammad Rais
spellingShingle	Mohd Isa, Noorain Abdelwahab, Siddig Ibrahim Mohan, Syam Abdul, Ahmad Bustaman Sukari, Mohd Aspollah Taha, Manal Mohamed Elhassan Syam, Suvitha Mohd Fauzi, Putri Narrima Cheah, Shiau Chuen Ahmad, Syahida Mustafa, Mohammad Rais In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
author_facet	Mohd Isa, Noorain Abdelwahab, Siddig Ibrahim Mohan, Syam Abdul, Ahmad Bustaman Sukari, Mohd Aspollah Taha, Manal Mohamed Elhassan Syam, Suvitha Mohd Fauzi, Putri Narrima Cheah, Shiau Chuen Ahmad, Syahida Mustafa, Mohammad Rais
author_sort	Mohd Isa, Noorain
title	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_short	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_full	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_fullStr	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_full_unstemmed	In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)
title_sort	in vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin a, a chalcone isolated from boesenbergia rotunda (l.) (fingerroot)
publisher	Associacao Brasileira de Divulgacao Cientifica
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/40428/1/In%20vitro%20anti-inflammatory%2C%20cytotoxic%20and%20antioxidant%20activities%20of%20boesenbergin%20A%2C%20a%20chalcone%20isolated%20from%20Boesenbergia%20rotunda%20%28L.%29%20%28fingerroot%29.pdf http://psasir.upm.edu.my/id/eprint/40428/ http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012000600008&lng=en&nrm=iso&tlng=en
_version_	1643832713271050240
score	13.211869

In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot)

Similar Items