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An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes

A gene (pMON9617; Chi2;1) identified by screening a tomato pistil cDNA library has been found to encode a protein similar in sequence to class II chitinases. Using a baculovirus expression system we show that the Chi2;1 protein is an active endochitinase. The Chi2;1 protein is most similar in sequen...

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Main Authors: Harikrishna, K., Jampates-Beale, Rachanee, Milligan, Stephen B., Gasser, Charles S.
Format: Article
Language:English
English
Published: Springer Verlag 1996
Online Access:http://psasir.upm.edu.my/id/eprint/39106/1/An%20endochitinase%20gene%20expressed%20at%20high%20levels.pdf
http://psasir.upm.edu.my/id/eprint/39106/7/BF00020802.pdf
http://psasir.upm.edu.my/id/eprint/39106/
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spelling my.upm.eprints.391062024-07-26T09:34:07Z http://psasir.upm.edu.my/id/eprint/39106/ An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes Harikrishna, K. Jampates-Beale, Rachanee Milligan, Stephen B. Gasser, Charles S. A gene (pMON9617; Chi2;1) identified by screening a tomato pistil cDNA library has been found to encode a protein similar in sequence to class II chitinases. Using a baculovirus expression system we show that the Chi2;1 protein is an active endochitinase. The Chi2;1 protein is most similar in sequence to a previously described stylar chitinase (SK2) isolated from potato. Both proteins lack the diagnostic N-terminal cysteine-rich regions and the C-terminal vacuolar targeting signals of class I chitinases and appear to define a novel type of class II endochitinases associated with flowers. Chi2;1 is expressed predominantly in floral organs and its expression within these organs is temporally regulated. The highest level of expression is found in the transmitting tissue of the style where Chi2;1 mRNA begins to accumulate just prior to anthesis. In vegetative tissue, low levels of Chi2;1 mRNA were detected, and these levels did not increase in response to wounding or treatment with ethephon. mRNA from Chi2;1 orthologs was not detected in most other angiosperms tested, even including some members of the Solanaceae, and it is therefore unlikely that Chi2;1 is essential for stylar function. A fragment containing 1.4 kilobase pairs of 5′-flanking DNA from the Chi2;1 gene was shown to drive high-level expression of an attached reporter gene in the styles of transgenic tomatoes. This fragment has utility for engineering expression of other coding regions in styles. Springer Verlag 1996-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/39106/1/An%20endochitinase%20gene%20expressed%20at%20high%20levels.pdf text en http://psasir.upm.edu.my/id/eprint/39106/7/BF00020802.pdf Harikrishna, K. and Jampates-Beale, Rachanee and Milligan, Stephen B. and Gasser, Charles S. (1996) An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes. Plant Molecular Biology Reporter, 30 (5). pp. 899-911. ISSN 0735-9640; ESSN:1572-9818 10.1007/BF00020802
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A gene (pMON9617; Chi2;1) identified by screening a tomato pistil cDNA library has been found to encode a protein similar in sequence to class II chitinases. Using a baculovirus expression system we show that the Chi2;1 protein is an active endochitinase. The Chi2;1 protein is most similar in sequence to a previously described stylar chitinase (SK2) isolated from potato. Both proteins lack the diagnostic N-terminal cysteine-rich regions and the C-terminal vacuolar targeting signals of class I chitinases and appear to define a novel type of class II endochitinases associated with flowers. Chi2;1 is expressed predominantly in floral organs and its expression within these organs is temporally regulated. The highest level of expression is found in the transmitting tissue of the style where Chi2;1 mRNA begins to accumulate just prior to anthesis. In vegetative tissue, low levels of Chi2;1 mRNA were detected, and these levels did not increase in response to wounding or treatment with ethephon. mRNA from Chi2;1 orthologs was not detected in most other angiosperms tested, even including some members of the Solanaceae, and it is therefore unlikely that Chi2;1 is essential for stylar function. A fragment containing 1.4 kilobase pairs of 5′-flanking DNA from the Chi2;1 gene was shown to drive high-level expression of an attached reporter gene in the styles of transgenic tomatoes. This fragment has utility for engineering expression of other coding regions in styles.
format Article
author Harikrishna, K.
Jampates-Beale, Rachanee
Milligan, Stephen B.
Gasser, Charles S.
spellingShingle Harikrishna, K.
Jampates-Beale, Rachanee
Milligan, Stephen B.
Gasser, Charles S.
An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
author_facet Harikrishna, K.
Jampates-Beale, Rachanee
Milligan, Stephen B.
Gasser, Charles S.
author_sort Harikrishna, K.
title An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
title_short An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
title_full An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
title_fullStr An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
title_full_unstemmed An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
title_sort endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
publisher Springer Verlag
publishDate 1996
url http://psasir.upm.edu.my/id/eprint/39106/1/An%20endochitinase%20gene%20expressed%20at%20high%20levels.pdf
http://psasir.upm.edu.my/id/eprint/39106/7/BF00020802.pdf
http://psasir.upm.edu.my/id/eprint/39106/
_version_ 1805889947115192320
score 13.211869

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