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Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures

Mesophyll cells of asparagus will undergo dedifferentiation when placed in culture medium. Cells may be mechanically isolated from asparagus cladodes rapidly and in sufficient quantities to enable a study of this switch in development at the microscopic level. During dedifferentiation a reproducible...

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Main Authors: Paul, E., Harikrishna, K., Fioroni, O., Draper, J.
Format: Article
Language:English
Published: Elsevier 1989
Online Access:http://psasir.upm.edu.my/id/eprint/38564/1/DEDIFFERENTIATION%20OF%20ASPARAGUS%20OFFICINALIS%20L.%20MESOPHYLL%20CELLS.pdf
http://psasir.upm.edu.my/id/eprint/38564/
http://www.sciencedirect.com/science/article/pii/0168945289902136
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spelling my.upm.eprints.385642016-01-11T06:57:49Z http://psasir.upm.edu.my/id/eprint/38564/ Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures Paul, E. Harikrishna, K. Fioroni, O. Draper, J. Mesophyll cells of asparagus will undergo dedifferentiation when placed in culture medium. Cells may be mechanically isolated from asparagus cladodes rapidly and in sufficient quantities to enable a study of this switch in development at the microscopic level. During dedifferentiation a reproducible, ordered sequence of events occurs that includes revocery from wound stress, reactivation of the cell cycle followed by cell expansion and cell division. Dedifferentiation in vitro is a complicated process which, at the cellular level, appears to resemble most aspects of dedifferentation during callus formation at wound sites in intact plants and large explants. Results from this intial study have been used to define developmental phases of dedifferentiation, which will be used in future molecular analyses of this system. Elsevier 1989 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38564/1/DEDIFFERENTIATION%20OF%20ASPARAGUS%20OFFICINALIS%20L.%20MESOPHYLL%20CELLS.pdf Paul, E. and Harikrishna, K. and Fioroni, O. and Draper, J. (1989) Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures. Plant Science, 65 (1). pp. 111-117. ISSN 0168-9452; ESSN: 1873-2259 http://www.sciencedirect.com/science/article/pii/0168945289902136 10.1016/0168-9452(89)90213-6
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Mesophyll cells of asparagus will undergo dedifferentiation when placed in culture medium. Cells may be mechanically isolated from asparagus cladodes rapidly and in sufficient quantities to enable a study of this switch in development at the microscopic level. During dedifferentiation a reproducible, ordered sequence of events occurs that includes revocery from wound stress, reactivation of the cell cycle followed by cell expansion and cell division. Dedifferentiation in vitro is a complicated process which, at the cellular level, appears to resemble most aspects of dedifferentation during callus formation at wound sites in intact plants and large explants. Results from this intial study have been used to define developmental phases of dedifferentiation, which will be used in future molecular analyses of this system.
format Article
author Paul, E.
Harikrishna, K.
Fioroni, O.
Draper, J.
spellingShingle Paul, E.
Harikrishna, K.
Fioroni, O.
Draper, J.
Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures
author_facet Paul, E.
Harikrishna, K.
Fioroni, O.
Draper, J.
author_sort Paul, E.
title Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures
title_short Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures
title_full Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures
title_fullStr Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures
title_full_unstemmed Dedifferentiation of Asparagus officinalis L. mesophyll cells during initiation of cell cultures
title_sort dedifferentiation of asparagus officinalis l. mesophyll cells during initiation of cell cultures
publisher Elsevier
publishDate 1989
url http://psasir.upm.edu.my/id/eprint/38564/1/DEDIFFERENTIATION%20OF%20ASPARAGUS%20OFFICINALIS%20L.%20MESOPHYLL%20CELLS.pdf
http://psasir.upm.edu.my/id/eprint/38564/
http://www.sciencedirect.com/science/article/pii/0168945289902136
_version_ 1643832174891237376
score 13.211869

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