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Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal

Heavy metals from industrial effluents and sewage contribute to serious water pollution in most developing countries. The constant penetration and contamination of heavy metals into natural water sources may substantially raise the chances of human exposure to these metals through ingestion, inhalat...

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Main Authors: Md Badrul Hisham, Nurul Hanisah, Ibrahim, Mohamad Faizal, Ramli, Norhayati, Abd. Aziz, Suraini
Format: Article
Language:English
Published: MDPI 2019
Online Access:http://psasir.upm.edu.my/id/eprint/38301/1/38301.pdf
http://psasir.upm.edu.my/id/eprint/38301/
https://www.mdpi.com/1420-3049/24/14/2617
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spelling my.upm.eprints.383012020-05-04T16:14:28Z http://psasir.upm.edu.my/id/eprint/38301/ Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal Md Badrul Hisham, Nurul Hanisah Ibrahim, Mohamad Faizal Ramli, Norhayati Abd. Aziz, Suraini Heavy metals from industrial effluents and sewage contribute to serious water pollution in most developing countries. The constant penetration and contamination of heavy metals into natural water sources may substantially raise the chances of human exposure to these metals through ingestion, inhalation, or skin contact, which could lead to liver damage, cancer, and other severe conditions in the long term. Biosurfactant as an efficient biological surface-active agent may provide an alternative solution for the removal of heavy metals from industrial wastes. Biosurfactants exhibit the properties of reducing surface and interfacial tension, stabilizing emulsions, promoting foaming, high selectivity, and specific activity at extreme temperatures, pH, and salinity, and the ability to be synthesized from renewable resources. This study aimed to produce biosurfactant from renewable feedstock, which is used cooking oil (UCO), by a local isolate, namely Bacillus sp. HIP3 for heavy metals removal. Bacillus sp. HIP3 is a Gram-positive isolate that gave the highest oil displacement area with the lowest surface tension, of 38 mN/m, after 7 days of culturing in mineral salt medium and 2% (v/v) UCO at a temperature of 30 °C and under agitation at 200 rpm. An extraction method, using chloroform:methanol (2:1) as the solvents, gave the highest biosurfactant yield, which was 9.5 g/L. High performance liquid chromatography (HPLC) analysis confirmed that the biosurfactant produced by Bacillus sp. HIP3 consists of a lipopeptide similar to standard surfactin. The biosurfactant was capable of removing 13.57%, 12.71%, 2.91%, 1.68%, and 0.7% of copper, lead, zinc, chromium, and cadmium, respectively, from artificially contaminated water, highlighting its potential for bioremediation. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38301/1/38301.pdf Md Badrul Hisham, Nurul Hanisah and Ibrahim, Mohamad Faizal and Ramli, Norhayati and Abd. Aziz, Suraini (2019) Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal. Molecules, 24 (14). art. no. 2617. pp. 1-16. ISSN 1420-3049 https://www.mdpi.com/1420-3049/24/14/2617 10.3390/molecules24142617
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Heavy metals from industrial effluents and sewage contribute to serious water pollution in most developing countries. The constant penetration and contamination of heavy metals into natural water sources may substantially raise the chances of human exposure to these metals through ingestion, inhalation, or skin contact, which could lead to liver damage, cancer, and other severe conditions in the long term. Biosurfactant as an efficient biological surface-active agent may provide an alternative solution for the removal of heavy metals from industrial wastes. Biosurfactants exhibit the properties of reducing surface and interfacial tension, stabilizing emulsions, promoting foaming, high selectivity, and specific activity at extreme temperatures, pH, and salinity, and the ability to be synthesized from renewable resources. This study aimed to produce biosurfactant from renewable feedstock, which is used cooking oil (UCO), by a local isolate, namely Bacillus sp. HIP3 for heavy metals removal. Bacillus sp. HIP3 is a Gram-positive isolate that gave the highest oil displacement area with the lowest surface tension, of 38 mN/m, after 7 days of culturing in mineral salt medium and 2% (v/v) UCO at a temperature of 30 °C and under agitation at 200 rpm. An extraction method, using chloroform:methanol (2:1) as the solvents, gave the highest biosurfactant yield, which was 9.5 g/L. High performance liquid chromatography (HPLC) analysis confirmed that the biosurfactant produced by Bacillus sp. HIP3 consists of a lipopeptide similar to standard surfactin. The biosurfactant was capable of removing 13.57%, 12.71%, 2.91%, 1.68%, and 0.7% of copper, lead, zinc, chromium, and cadmium, respectively, from artificially contaminated water, highlighting its potential for bioremediation.
format Article
author Md Badrul Hisham, Nurul Hanisah
Ibrahim, Mohamad Faizal
Ramli, Norhayati
Abd. Aziz, Suraini
spellingShingle Md Badrul Hisham, Nurul Hanisah
Ibrahim, Mohamad Faizal
Ramli, Norhayati
Abd. Aziz, Suraini
Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal
author_facet Md Badrul Hisham, Nurul Hanisah
Ibrahim, Mohamad Faizal
Ramli, Norhayati
Abd. Aziz, Suraini
author_sort Md Badrul Hisham, Nurul Hanisah
title Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal
title_short Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal
title_full Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal
title_fullStr Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal
title_full_unstemmed Production of biosurfactant produced from used cooking oil by Bacillus sp. HIP3 for heavy metals removal
title_sort production of biosurfactant produced from used cooking oil by bacillus sp. hip3 for heavy metals removal
publisher MDPI
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/38301/1/38301.pdf
http://psasir.upm.edu.my/id/eprint/38301/
https://www.mdpi.com/1420-3049/24/14/2617
_version_ 1665895973106221056
score 13.211869

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