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Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect

Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and...

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Main Authors: Ng, Wai Hoe, Yong, Yoke Keong, Ramasamy, Rajesh, Ngalim, Siti Hawa, Lim, Vuanghao, Shaharuddin, Bakiah, Tan, Jun Jie
Format: Article
Language:English
Published: MDPI 2019
Online Access:http://psasir.upm.edu.my/id/eprint/38220/1/38220.pdf
http://psasir.upm.edu.my/id/eprint/38220/
https://www.mdpi.com/1422-0067/20/22/5519
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spelling my.upm.eprints.382202020-05-04T15:59:52Z http://psasir.upm.edu.my/id/eprint/38220/ Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect Ng, Wai Hoe Yong, Yoke Keong Ramasamy, Rajesh Ngalim, Siti Hawa Lim, Vuanghao Shaharuddin, Bakiah Tan, Jun Jie Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and studied the effects of human Wharton’s Jelly-derived mesenchymal stem cells (MSCs) on the growth kinetics and cardiac differentiation of aged CCs in vitro. CCs were isolated from 4-week- and 18-month-old C57/BL6N mice and were directly co-cultured with MSCs or separated by transwell insert. Clonogenically expanded aged CCs showed comparable telomere length to young CCs. However, these cells showed lower Gata4, Nkx2.5, and Sox2 gene expressions, with changes of 2.4, 3767.0, and 4.9 folds, respectively. Direct co-culture of both cells increased aged CC migration, which repopulated 54.6 ± 4.4% of the gap area as compared to aged CCs with MSCs in transwell (42.9 ± 2.6%) and CCs without MSCs (44.7 ± 2.5%). Both direct and transwell co-culture improved proliferation in aged CCs by 15.0% and 16.4%, respectively, as traced using carboxyfluorescein succinimidyl ester (CFSE) for three days. These data suggest that MSCs can improve the growth kinetics of aged CCs. CCs retaining intact telomere are present in old hearts and could be obtained based on their self-renewing capability. Although these aged CCs with reduced growth kinetics are improved by MSCs via cell–cell contact, the effect is minimal. MDPI 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38220/1/38220.pdf Ng, Wai Hoe and Yong, Yoke Keong and Ramasamy, Rajesh and Ngalim, Siti Hawa and Lim, Vuanghao and Shaharuddin, Bakiah and Tan, Jun Jie (2019) Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect. International Journal of Molecular Sciences, 20 (22). art. no. 5519. pp. 1-20. ISSN 1661-6596; ESSN: 1422-0067 https://www.mdpi.com/1422-0067/20/22/5519 10.3390/ijms20225519
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Cardiac c-kit cells show promise in regenerating an injured heart. While heart disease commonly affects elderly patients, it is unclear if autologous cardiac c-kit cells are functionally competent and applicable to these patients. This study characterised cardiac c-kit cells (CCs) from aged mice and studied the effects of human Wharton’s Jelly-derived mesenchymal stem cells (MSCs) on the growth kinetics and cardiac differentiation of aged CCs in vitro. CCs were isolated from 4-week- and 18-month-old C57/BL6N mice and were directly co-cultured with MSCs or separated by transwell insert. Clonogenically expanded aged CCs showed comparable telomere length to young CCs. However, these cells showed lower Gata4, Nkx2.5, and Sox2 gene expressions, with changes of 2.4, 3767.0, and 4.9 folds, respectively. Direct co-culture of both cells increased aged CC migration, which repopulated 54.6 ± 4.4% of the gap area as compared to aged CCs with MSCs in transwell (42.9 ± 2.6%) and CCs without MSCs (44.7 ± 2.5%). Both direct and transwell co-culture improved proliferation in aged CCs by 15.0% and 16.4%, respectively, as traced using carboxyfluorescein succinimidyl ester (CFSE) for three days. These data suggest that MSCs can improve the growth kinetics of aged CCs. CCs retaining intact telomere are present in old hearts and could be obtained based on their self-renewing capability. Although these aged CCs with reduced growth kinetics are improved by MSCs via cell–cell contact, the effect is minimal.
format Article
author Ng, Wai Hoe
Yong, Yoke Keong
Ramasamy, Rajesh
Ngalim, Siti Hawa
Lim, Vuanghao
Shaharuddin, Bakiah
Tan, Jun Jie
spellingShingle Ng, Wai Hoe
Yong, Yoke Keong
Ramasamy, Rajesh
Ngalim, Siti Hawa
Lim, Vuanghao
Shaharuddin, Bakiah
Tan, Jun Jie
Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
author_facet Ng, Wai Hoe
Yong, Yoke Keong
Ramasamy, Rajesh
Ngalim, Siti Hawa
Lim, Vuanghao
Shaharuddin, Bakiah
Tan, Jun Jie
author_sort Ng, Wai Hoe
title Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
title_short Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
title_full Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
title_fullStr Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
title_full_unstemmed Human Wharton's Jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
title_sort human wharton's jelly-derived mesenchymal stem cells minimally improve the growth kinetics and cardiomyocyte differentiation of aged murine cardiac c-kit cells in in vitro without rejuvenating effect
publisher MDPI
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/38220/1/38220.pdf
http://psasir.upm.edu.my/id/eprint/38220/
https://www.mdpi.com/1422-0067/20/22/5519
_version_ 1665895965267066880
score 13.211869

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