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Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method, in combination with a mixed matrix membrane microextraction method for the quantification of nonsteroidal anti-inflammatory drugs (NSAIDs) in environmental water samples, is reported. The extraction device was p...

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Main Authors: Ganesan, Thipashini, Mukhtar, Nurul Hazirah, Lim, Hong Ngee, See, Hong Heng
Format: Article
Language:English
Published: MDPI 2020
Online Access:http://psasir.upm.edu.my/id/eprint/38122/1/38122.pdf
http://psasir.upm.edu.my/id/eprint/38122/
https://www.mdpi.com/2297-8739/7/1/19
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spelling my.upm.eprints.381222020-05-03T22:52:38Z http://psasir.upm.edu.my/id/eprint/38122/ Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples Ganesan, Thipashini Mukhtar, Nurul Hazirah Lim, Hong Ngee See, Hong Heng An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method, in combination with a mixed matrix membrane microextraction method for the quantification of nonsteroidal anti-inflammatory drugs (NSAIDs) in environmental water samples, is reported. The extraction device was prepared by casting well-dispersed polymeric bonded octadecyl (C18) particles in a cellulose triacetate matrix solution onto commercially available 200 μL micropipette tips. The membrane formed contains 25% of the adsorbent loading amount and was firmly attached to the inner wall of the membrane tip. The dynamic extraction was performed by withdrawing and dispensing the sample solution through the tip device for effective analyte adsorption, followed by the analyte desorption process into 40 μL of methanol and acetonitrile (1:1) prior to UPLC–MS/MS analysis. NSAIDs—namely diclofenac, ibuprofen, indoprofen, naproxen and sulindac—were chosen as targeted analytes. Several extraction parameters were comprehensively optimized, including sample pH value, ionic strength, dynamic extraction cycle, desorption solvent and desorption time. The optimized conditions demonstrated a linear range from 0.25 to 500 ng L−1, with correlation coefficients (r2) from 0.9988 to 0.9992 and detection limits ranging from 0.08 to 0.40 ng L−1. The recoveries of the spiked water samples were between 92% and 99% and exhibited excellent precision relative to standard deviations (RSDs ≤ 4.9%), and enrichment factors (EFs) were at 201–249 for the developed approach. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38122/1/38122.pdf Ganesan, Thipashini and Mukhtar, Nurul Hazirah and Lim, Hong Ngee and See, Hong Heng (2020) Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples. Separations, 7 (1). art. no. 19. pp. 1-10. ISSN 2297-8739 https://www.mdpi.com/2297-8739/7/1/19 10.3390/separations7010019
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method, in combination with a mixed matrix membrane microextraction method for the quantification of nonsteroidal anti-inflammatory drugs (NSAIDs) in environmental water samples, is reported. The extraction device was prepared by casting well-dispersed polymeric bonded octadecyl (C18) particles in a cellulose triacetate matrix solution onto commercially available 200 μL micropipette tips. The membrane formed contains 25% of the adsorbent loading amount and was firmly attached to the inner wall of the membrane tip. The dynamic extraction was performed by withdrawing and dispensing the sample solution through the tip device for effective analyte adsorption, followed by the analyte desorption process into 40 μL of methanol and acetonitrile (1:1) prior to UPLC–MS/MS analysis. NSAIDs—namely diclofenac, ibuprofen, indoprofen, naproxen and sulindac—were chosen as targeted analytes. Several extraction parameters were comprehensively optimized, including sample pH value, ionic strength, dynamic extraction cycle, desorption solvent and desorption time. The optimized conditions demonstrated a linear range from 0.25 to 500 ng L−1, with correlation coefficients (r2) from 0.9988 to 0.9992 and detection limits ranging from 0.08 to 0.40 ng L−1. The recoveries of the spiked water samples were between 92% and 99% and exhibited excellent precision relative to standard deviations (RSDs ≤ 4.9%), and enrichment factors (EFs) were at 201–249 for the developed approach.
format Article
author Ganesan, Thipashini
Mukhtar, Nurul Hazirah
Lim, Hong Ngee
See, Hong Heng
spellingShingle Ganesan, Thipashini
Mukhtar, Nurul Hazirah
Lim, Hong Ngee
See, Hong Heng
Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
author_facet Ganesan, Thipashini
Mukhtar, Nurul Hazirah
Lim, Hong Ngee
See, Hong Heng
author_sort Ganesan, Thipashini
title Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
title_short Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
title_full Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
title_fullStr Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
title_full_unstemmed Mixed matrix membrane tip extraction coupled with UPLC–MS/MS for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
title_sort mixed matrix membrane tip extraction coupled with uplc–ms/ms for the monitoring of nonsteroidal anti-inflammatory drugs in water samples
publisher MDPI
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/38122/1/38122.pdf
http://psasir.upm.edu.my/id/eprint/38122/
https://www.mdpi.com/2297-8739/7/1/19
_version_ 1665895955685179392
score 13.211869

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