Cover Image

In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion

Background: Edible birds’ nest (EBN) is reported to be antioxidant-rich. However, the fate of its antioxidants after oral consumption is not yet reported. To explore this, we hypothesized that EBN antioxidants are released from their matrix when subjected to in vitro simulated gastrointestinal diges...

Full description

Saved in:
Bibliographic Details
Main Authors: Yida, Zhang, Imam, Mustapha Umar, Ismail, Maznah
Format: Article
Language:English
Published: BioMed Central 2014
Online Access:http://psasir.upm.edu.my/id/eprint/38006/1/38006.pdf
http://psasir.upm.edu.my/id/eprint/38006/
http://www.biomedcentral.com/1472-6882/14/468/abstract
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.38006
record_format eprints
spelling my.upm.eprints.380062015-12-18T07:04:11Z http://psasir.upm.edu.my/id/eprint/38006/ In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion Yida, Zhang Imam, Mustapha Umar Ismail, Maznah Background: Edible birds’ nest (EBN) is reported to be antioxidant-rich. However, the fate of its antioxidants after oral consumption is not yet reported. To explore this, we hypothesized that EBN antioxidants are released from their matrix when subjected to in vitro simulated gastrointestinal digestion. Methods: EBN samples were extracted using hot water (100°C) with or without subsequent sequential enzymatic digestion using pepsin (10,000 units), pancreatin (36 mg) and bile extracts (112.5 mg). Additionally, pH changes (8.9 to 2 and back to 8.9) similar to the gut were applied, and a 10 KDa dialysis tubing was used to simulate gut absorption. The antioxidant capacities of the water extracts of EBN before and after digestion were then determined using ABTS and oxygen radical absorbance capacity (ORAC) assays, while the protective effects of the EBN samples against hydrogen peroxide-induced toxicity in HEPG2 cells were determined using MTT assay and acridine orange (AO)/propidium iodide (PI) staining. Results: Antioxidant assays (ABTS and ORAC) showed that the undigested EBN water extract had little antioxidant activity (1 and 1%, respectively at 1000 μg/mL) while at similar concentrations the digested samples had significantly (p < 0.05) enhanced antioxidant activities, for samples inside (38 and 50%, respectively at 1000 μg/mL) and outside (36 and 50%, respectively at 1000 μg/mL) the dialysis tubing, representing absorbed and unabsorbed samples, respectively. Cell viability and toxicity assays also suggested that the EBN extracts were non-toxic to HEPG2 cells (cell viabilities of over 80% at 1000 μg/mL), while AOPI showed that the extracts protected HEPG2 cells from hydrogen peroxide induced-toxicity. Conclusions: Based on the findings, it is likely that EBN bioactives are released from their matrix when digested in the gut and then absorbed through the gut by passive-mediated transport to exert their functional effects. However, there is need to confirm these findings using in vivo systems to determine their clinical significance. BioMed Central 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38006/1/38006.pdf Yida, Zhang and Imam, Mustapha Umar and Ismail, Maznah (2014) In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion. BMC Complementary and Alternative Medicine, 14. art. no. 468. pp. 1-7. ISSN 1472-6882 http://www.biomedcentral.com/1472-6882/14/468/abstract 10.1186/1472-6882-14-468
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Edible birds’ nest (EBN) is reported to be antioxidant-rich. However, the fate of its antioxidants after oral consumption is not yet reported. To explore this, we hypothesized that EBN antioxidants are released from their matrix when subjected to in vitro simulated gastrointestinal digestion. Methods: EBN samples were extracted using hot water (100°C) with or without subsequent sequential enzymatic digestion using pepsin (10,000 units), pancreatin (36 mg) and bile extracts (112.5 mg). Additionally, pH changes (8.9 to 2 and back to 8.9) similar to the gut were applied, and a 10 KDa dialysis tubing was used to simulate gut absorption. The antioxidant capacities of the water extracts of EBN before and after digestion were then determined using ABTS and oxygen radical absorbance capacity (ORAC) assays, while the protective effects of the EBN samples against hydrogen peroxide-induced toxicity in HEPG2 cells were determined using MTT assay and acridine orange (AO)/propidium iodide (PI) staining. Results: Antioxidant assays (ABTS and ORAC) showed that the undigested EBN water extract had little antioxidant activity (1 and 1%, respectively at 1000 μg/mL) while at similar concentrations the digested samples had significantly (p < 0.05) enhanced antioxidant activities, for samples inside (38 and 50%, respectively at 1000 μg/mL) and outside (36 and 50%, respectively at 1000 μg/mL) the dialysis tubing, representing absorbed and unabsorbed samples, respectively. Cell viability and toxicity assays also suggested that the EBN extracts were non-toxic to HEPG2 cells (cell viabilities of over 80% at 1000 μg/mL), while AOPI showed that the extracts protected HEPG2 cells from hydrogen peroxide induced-toxicity. Conclusions: Based on the findings, it is likely that EBN bioactives are released from their matrix when digested in the gut and then absorbed through the gut by passive-mediated transport to exert their functional effects. However, there is need to confirm these findings using in vivo systems to determine their clinical significance.
format Article
author Yida, Zhang
Imam, Mustapha Umar
Ismail, Maznah
spellingShingle Yida, Zhang
Imam, Mustapha Umar
Ismail, Maznah
In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion
author_facet Yida, Zhang
Imam, Mustapha Umar
Ismail, Maznah
author_sort Yida, Zhang
title In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion
title_short In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion
title_full In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion
title_fullStr In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion
title_full_unstemmed In vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion
title_sort in vitro bioaccessibility and antioxidant properties of edible bird's nest following simulated human gastro-intestinal digestion
publisher BioMed Central
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/38006/1/38006.pdf
http://psasir.upm.edu.my/id/eprint/38006/
http://www.biomedcentral.com/1472-6882/14/468/abstract
_version_ 1643832122887110656
score 13.211869

Similar Items