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In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract

Vernonia amygdalina is a strong natural antioxidant that possessed various medicinal properties. In this study, the spray-dried water extract of V. amygdalina was evaluated for its in vitro antioxidant capacity and in vivo hepatoprotective effect against alcoholic-mediated liver damage. Total phenol...

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Main Authors: Ho, Wan Yong, Yeap, Swee Keong, Liang, Woon San, Beh, Boon Kee, Mohamad, Nurul Elyani, Mohamed Alitheen, Noorjahan Banu
Format: Article
Language:English
Published: Faculty of Pharmacy, University of Karachi 2015
Online Access:http://psasir.upm.edu.my/id/eprint/38003/1/In%20vitro%20antioxidant%20and%20in%20vivo%20hepatoprotective%20effect%20on%20ethanol-mediated%20liver%20damage%20of%20spray%20dried%20Vernonia%20amygdalina%20water%20extract.pdf
http://psasir.upm.edu.my/id/eprint/38003/
http://www.pjps.pk/?page_id=258
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spelling my.upm.eprints.380032015-12-15T03:41:14Z http://psasir.upm.edu.my/id/eprint/38003/ In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract Ho, Wan Yong Yeap, Swee Keong Liang, Woon San Beh, Boon Kee Mohamad, Nurul Elyani Mohamed Alitheen, Noorjahan Banu Vernonia amygdalina is a strong natural antioxidant that possessed various medicinal properties. In this study, the spray-dried water extract of V. amygdalina was evaluated for its in vitro antioxidant capacity and in vivo hepatoprotective effect against alcoholic-mediated liver damage. Total phenolic and flavonoid content of spray-dried V. amygdalina water extract were determined. Liver enzyme profiles, liver antioxidant level and nitric oxide level were evaluated in alcohol-induced liver injured mice or co-supplement with spray-dried V. amydalina. Water extract of spraydried V. amygalina that contained phenolic content of 24.8±1.5 mg/g gallic acid equivalent and total flavonoid content of 25.7±1.3 mg/g catechin equivalent was able to inhibit 50% of xanthine and tyrosinase oxidation at 170 µg/mL and 2 mg/mL, respectively. On the other hand, extracts at both 10 and 50 mg/kg body weight were able to reduce the levels of Alanine transaminase (ALT), Alkaline phosphatase (ALP), Aspartate transaminase (AST), triglyceride and total bilirubin content inthe alcohol-mediated liver injury in mice. Furthermore, it also helped to increase levels of Superoxide dismutase (SOD), Ferric reducing ability of plasma (FRAP) and reduce the levels of Nitric oxide (NO) and Malondialdehyde (MDA) in the liver of the treated mice. These resultssuggestedthat water extract of spray-dried V. amygdalina exhibited liver protective effect, which could be contributed by its antioxidant properties. Faculty of Pharmacy, University of Karachi 2015-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38003/1/In%20vitro%20antioxidant%20and%20in%20vivo%20hepatoprotective%20effect%20on%20ethanol-mediated%20liver%20damage%20of%20spray%20dried%20Vernonia%20amygdalina%20water%20extract.pdf Ho, Wan Yong and Yeap, Swee Keong and Liang, Woon San and Beh, Boon Kee and Mohamad, Nurul Elyani and Mohamed Alitheen, Noorjahan Banu (2015) In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract. Pakistan Journal of Pharmaceutical Sciences, 28 (1). pp. 15-22. ISSN 1011-601X http://www.pjps.pk/?page_id=258
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Vernonia amygdalina is a strong natural antioxidant that possessed various medicinal properties. In this study, the spray-dried water extract of V. amygdalina was evaluated for its in vitro antioxidant capacity and in vivo hepatoprotective effect against alcoholic-mediated liver damage. Total phenolic and flavonoid content of spray-dried V. amygdalina water extract were determined. Liver enzyme profiles, liver antioxidant level and nitric oxide level were evaluated in alcohol-induced liver injured mice or co-supplement with spray-dried V. amydalina. Water extract of spraydried V. amygalina that contained phenolic content of 24.8±1.5 mg/g gallic acid equivalent and total flavonoid content of 25.7±1.3 mg/g catechin equivalent was able to inhibit 50% of xanthine and tyrosinase oxidation at 170 µg/mL and 2 mg/mL, respectively. On the other hand, extracts at both 10 and 50 mg/kg body weight were able to reduce the levels of Alanine transaminase (ALT), Alkaline phosphatase (ALP), Aspartate transaminase (AST), triglyceride and total bilirubin content inthe alcohol-mediated liver injury in mice. Furthermore, it also helped to increase levels of Superoxide dismutase (SOD), Ferric reducing ability of plasma (FRAP) and reduce the levels of Nitric oxide (NO) and Malondialdehyde (MDA) in the liver of the treated mice. These resultssuggestedthat water extract of spray-dried V. amygdalina exhibited liver protective effect, which could be contributed by its antioxidant properties.
format Article
author Ho, Wan Yong
Yeap, Swee Keong
Liang, Woon San
Beh, Boon Kee
Mohamad, Nurul Elyani
Mohamed Alitheen, Noorjahan Banu
spellingShingle Ho, Wan Yong
Yeap, Swee Keong
Liang, Woon San
Beh, Boon Kee
Mohamad, Nurul Elyani
Mohamed Alitheen, Noorjahan Banu
In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract
author_facet Ho, Wan Yong
Yeap, Swee Keong
Liang, Woon San
Beh, Boon Kee
Mohamad, Nurul Elyani
Mohamed Alitheen, Noorjahan Banu
author_sort Ho, Wan Yong
title In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract
title_short In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract
title_full In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract
title_fullStr In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract
title_full_unstemmed In vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried Vernonia amygdalina water extract
title_sort in vitro antioxidant and in vivo hepatoprotective effect on ethanol-mediated liver damage of spray dried vernonia amygdalina water extract
publisher Faculty of Pharmacy, University of Karachi
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/38003/1/In%20vitro%20antioxidant%20and%20in%20vivo%20hepatoprotective%20effect%20on%20ethanol-mediated%20liver%20damage%20of%20spray%20dried%20Vernonia%20amygdalina%20water%20extract.pdf
http://psasir.upm.edu.my/id/eprint/38003/
http://www.pjps.pk/?page_id=258
_version_ 1643832121998966784
score 13.211869

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