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Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor

In this study, banana waste was used to investigate its elicitation potential for induced production of α-amylase from Bacillus licheniformis DSM-1969. Initially, six different media were investigated to select the composition with optimal yield. A comparison of the fermentations in the stirred ferm...

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Main Authors: Bashir, Rashida, Syed, Quratulain, Mumtaz, Muhammad Waseem, Anwar, Farooq, Saari, Nazamid, Adnan, Ahmad
Format: Article
Published: College of Natural Resources, North Carolina State University 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37808/
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6791_Bashir_Growth_Kinetics_Purification_Bacillus
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spelling my.upm.eprints.378082015-09-15T14:13:19Z http://psasir.upm.edu.my/id/eprint/37808/ Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor Bashir, Rashida Syed, Quratulain Mumtaz, Muhammad Waseem Anwar, Farooq Saari, Nazamid Adnan, Ahmad In this study, banana waste was used to investigate its elicitation potential for induced production of α-amylase from Bacillus licheniformis DSM-1969. Initially, six different media were investigated to select the composition with optimal yield. A comparison of the fermentations in the stirred fermenter or shake flasks revealed that B. licheniformis DSM-1969 was more active to synthesize α-amylase in the fermenter as compared to the shake flask. In the shake flask during the exponential phase, the specific growth rate, generation time, and number of generations were 0.19 h-1, 3.48 h-1, and 5.16 h-1, respectively, whereas in the stirred fermenter the above values were 0.3 h-1, 2.31 h-1 and 5.21 h-1, respectively. A significant difference was recorded in the specific substrate uptake rate and biomass growth yield during the exponential phase in the stirred fermenter in comparison to the shake flask. The enzyme was purified by ion-exchange chromatography using fast protein liquid chromatography (FPLC). α-amylase was purified 3.9 fold with a specific activity of 38.8 U/mg and molecular weight of 62 kDa. Characterization revealed that purified α-amylase remained stable over a broad pH and temperature range as compared to the crude enzyme. Activity of this novel extra thermo-stable α-amylase was stimulated to variable extents by Zn2+, Co2+, and Mn2+, whereas EDTA and Hg2+ showed inhibitory effects. College of Natural Resources, North Carolina State University 2014 Article PeerReviewed Bashir, Rashida and Syed, Quratulain and Mumtaz, Muhammad Waseem and Anwar, Farooq and Saari, Nazamid and Adnan, Ahmad (2014) Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor. BioResources, 9 (4). pp. 6791-6804. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6791_Bashir_Growth_Kinetics_Purification_Bacillus
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description In this study, banana waste was used to investigate its elicitation potential for induced production of α-amylase from Bacillus licheniformis DSM-1969. Initially, six different media were investigated to select the composition with optimal yield. A comparison of the fermentations in the stirred fermenter or shake flasks revealed that B. licheniformis DSM-1969 was more active to synthesize α-amylase in the fermenter as compared to the shake flask. In the shake flask during the exponential phase, the specific growth rate, generation time, and number of generations were 0.19 h-1, 3.48 h-1, and 5.16 h-1, respectively, whereas in the stirred fermenter the above values were 0.3 h-1, 2.31 h-1 and 5.21 h-1, respectively. A significant difference was recorded in the specific substrate uptake rate and biomass growth yield during the exponential phase in the stirred fermenter in comparison to the shake flask. The enzyme was purified by ion-exchange chromatography using fast protein liquid chromatography (FPLC). α-amylase was purified 3.9 fold with a specific activity of 38.8 U/mg and molecular weight of 62 kDa. Characterization revealed that purified α-amylase remained stable over a broad pH and temperature range as compared to the crude enzyme. Activity of this novel extra thermo-stable α-amylase was stimulated to variable extents by Zn2+, Co2+, and Mn2+, whereas EDTA and Hg2+ showed inhibitory effects.
format Article
author Bashir, Rashida
Syed, Quratulain
Mumtaz, Muhammad Waseem
Anwar, Farooq
Saari, Nazamid
Adnan, Ahmad
spellingShingle Bashir, Rashida
Syed, Quratulain
Mumtaz, Muhammad Waseem
Anwar, Farooq
Saari, Nazamid
Adnan, Ahmad
Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor
author_facet Bashir, Rashida
Syed, Quratulain
Mumtaz, Muhammad Waseem
Anwar, Farooq
Saari, Nazamid
Adnan, Ahmad
author_sort Bashir, Rashida
title Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor
title_short Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor
title_full Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor
title_fullStr Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor
title_full_unstemmed Growth kinetics, purification and characterization of α-amylase produced from Bacillus licheniformis DSM-1969 using lignocellulosic banana waste as an elicitor
title_sort growth kinetics, purification and characterization of α-amylase produced from bacillus licheniformis dsm-1969 using lignocellulosic banana waste as an elicitor
publisher College of Natural Resources, North Carolina State University
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/37808/
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_4_6791_Bashir_Growth_Kinetics_Purification_Bacillus
_version_ 1643832068015128576
score 13.211869

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