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Functional characterization of sesquiterpene synthase from Polygonum minus

Polygonum minus is an aromatic plant, which contains high abundance of terpenoids, especially the sesquiterpenes C15H24. Sesquiterpenes were believed to contribute to the many useful biological properties in plants. This study aimed to functionally characterize a full length sesquiterpene synthase g...

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Main Authors:	Ee, Su Fang, Mohamed Hussein, Zeti Azura, Othman, Roohaida, Shaharuddin, Noor Azmi, Ismail, Ismanizan, Zainal, Zamri
Format:	Article
Published:	Hindawi Publishing Corporation 2014
Online Access:	http://psasir.upm.edu.my/id/eprint/37722/ http://www.hindawi.com/journals/tswj/2014/840592/
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id	my.upm.eprints.37722
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spelling	my.upm.eprints.377222015-09-18T12:15:43Z http://psasir.upm.edu.my/id/eprint/37722/ Functional characterization of sesquiterpene synthase from Polygonum minus Ee, Su Fang Mohamed Hussein, Zeti Azura Othman, Roohaida Shaharuddin, Noor Azmi Ismail, Ismanizan Zainal, Zamri Polygonum minus is an aromatic plant, which contains high abundance of terpenoids, especially the sesquiterpenes C15H24. Sesquiterpenes were believed to contribute to the many useful biological properties in plants. This study aimed to functionally characterize a full length sesquiterpene synthase gene from P. minus. P. minus sesquiterpene synthase (PmSTS) has a complete open reading frame (ORF) of 1689 base pairs encoding a 562 amino acid protein. Similar to other sesquiterpene synthases, PmSTS has two large domains: the N-terminal domain and the C-terminal metal-binding domain. It also consists of three conserved motifs: the DDXXD, NSE/DTE, and RXR. A three-dimensional protein model for PmSTS built clearly distinguished the two main domains, where conserved motifs were highlighted. We also constructed a phylogenetic tree, which showed that PmSTS belongs to the angiosperm sesquiterpene synthase subfamily Tps-a. To examine the function of PmSTS, we expressed this gene in Arabidopsis thaliana. Two transgenic lines, designated as OE3 and OE7, were further characterized, both molecularly and functionally. The transgenic plants demonstrated smaller basal rosette leaves, shorter and fewer flowering stems, and fewer seeds compared to wild type plants. Gas chromatography-mass spectrometry analysis of the transgenic plants showed that PmSTS was responsible for the production of β-sesquiphellandrene. Hindawi Publishing Corporation 2014 Article PeerReviewed Ee, Su Fang and Mohamed Hussein, Zeti Azura and Othman, Roohaida and Shaharuddin, Noor Azmi and Ismail, Ismanizan and Zainal, Zamri (2014) Functional characterization of sesquiterpene synthase from Polygonum minus. The Scientific World Journal, 2014. art. no. 840592. pp. 1-11. ISSN 2356-6140; ESSN: 1537-744X http://www.hindawi.com/journals/tswj/2014/840592/ 10.1155/2014/840592
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Polygonum minus is an aromatic plant, which contains high abundance of terpenoids, especially the sesquiterpenes C15H24. Sesquiterpenes were believed to contribute to the many useful biological properties in plants. This study aimed to functionally characterize a full length sesquiterpene synthase gene from P. minus. P. minus sesquiterpene synthase (PmSTS) has a complete open reading frame (ORF) of 1689 base pairs encoding a 562 amino acid protein. Similar to other sesquiterpene synthases, PmSTS has two large domains: the N-terminal domain and the C-terminal metal-binding domain. It also consists of three conserved motifs: the DDXXD, NSE/DTE, and RXR. A three-dimensional protein model for PmSTS built clearly distinguished the two main domains, where conserved motifs were highlighted. We also constructed a phylogenetic tree, which showed that PmSTS belongs to the angiosperm sesquiterpene synthase subfamily Tps-a. To examine the function of PmSTS, we expressed this gene in Arabidopsis thaliana. Two transgenic lines, designated as OE3 and OE7, were further characterized, both molecularly and functionally. The transgenic plants demonstrated smaller basal rosette leaves, shorter and fewer flowering stems, and fewer seeds compared to wild type plants. Gas chromatography-mass spectrometry analysis of the transgenic plants showed that PmSTS was responsible for the production of β-sesquiphellandrene.
format	Article
author	Ee, Su Fang Mohamed Hussein, Zeti Azura Othman, Roohaida Shaharuddin, Noor Azmi Ismail, Ismanizan Zainal, Zamri
spellingShingle	Ee, Su Fang Mohamed Hussein, Zeti Azura Othman, Roohaida Shaharuddin, Noor Azmi Ismail, Ismanizan Zainal, Zamri Functional characterization of sesquiterpene synthase from Polygonum minus
author_facet	Ee, Su Fang Mohamed Hussein, Zeti Azura Othman, Roohaida Shaharuddin, Noor Azmi Ismail, Ismanizan Zainal, Zamri
author_sort	Ee, Su Fang
title	Functional characterization of sesquiterpene synthase from Polygonum minus
title_short	Functional characterization of sesquiterpene synthase from Polygonum minus
title_full	Functional characterization of sesquiterpene synthase from Polygonum minus
title_fullStr	Functional characterization of sesquiterpene synthase from Polygonum minus
title_full_unstemmed	Functional characterization of sesquiterpene synthase from Polygonum minus
title_sort	functional characterization of sesquiterpene synthase from polygonum minus
publisher	Hindawi Publishing Corporation
publishDate	2014
url	http://psasir.upm.edu.my/id/eprint/37722/ http://www.hindawi.com/journals/tswj/2014/840592/
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score	13.211869

Functional characterization of sesquiterpene synthase from Polygonum minus

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