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Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract

Clausena excavata (Lour.), locally known as “Kemantu hitam,” is a common plant in Malaysian folklore medicine. This study evaluated the antioxidant properties of the solvent extracts of C. excavata leaves and determined the acute toxicity of methanolic extract C. excavata (MECE) leaves in Sprague-Da...

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Main Authors: Albaayit, Shaymaa Fadhel Abbas, Abba, Yusuf, Abdullah, Rasedee, Abdullah, Noorlidah
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37520/1/37520.pdf
http://psasir.upm.edu.my/id/eprint/37520/
http://www.hindawi.com/journals/ecam/2014/975450/abs/
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spelling my.upm.eprints.375202015-12-18T02:52:50Z http://psasir.upm.edu.my/id/eprint/37520/ Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract Albaayit, Shaymaa Fadhel Abbas Abba, Yusuf Abdullah, Rasedee Abdullah, Noorlidah Clausena excavata (Lour.), locally known as “Kemantu hitam,” is a common plant in Malaysian folklore medicine. This study evaluated the antioxidant properties of the solvent extracts of C. excavata leaves and determined the acute toxicity of methanolic extract C. excavata (MECE) leaves in Sprague-Dawley rats. Harvested leaves were dried and subjected to solvent extraction using petroleum ether, chloroform, ethyl acetate and methanol in succession. The antioxidant activity of each extract was determined using the ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl dihydrazyl (DPPH) radical scavenging activity. The total phenolic content (TPC) and total flavonoids content (TFC) were estimated by Folin-Ciocalteu and ethanolic aluminium chloride method, respectively. The chloroform extract was found to be highest in flavonoid content, while the methanolic extract showed the highest TPC and antioxidant activity. There was no mortality in rats treated with MECE leaves even at a high dose of 5000 mg/kg body weight. However, the MECE leaves produced mild to moderate pathological changes in the liver and kidneys, shown by mild degenerative changes and leucocyte infiltration. The extract did not affect the haematological parameters or relative weights of the liver or kidneys. Overall, the MECE leaves have potent antioxidant activity and are presumed safe to be used orally as health-promoting product at low to moderate doses. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37520/1/37520.pdf Albaayit, Shaymaa Fadhel Abbas and Abba, Yusuf and Abdullah, Rasedee and Abdullah, Noorlidah (2014) Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract. Evidence-Based Complementary and Alternative Medicine, 2014. art. no. 975450. pp. 1-10. ISSN 1741-427X; ESSN: 1741-4288 http://www.hindawi.com/journals/ecam/2014/975450/abs/ 10.1155/2014/975450
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Clausena excavata (Lour.), locally known as “Kemantu hitam,” is a common plant in Malaysian folklore medicine. This study evaluated the antioxidant properties of the solvent extracts of C. excavata leaves and determined the acute toxicity of methanolic extract C. excavata (MECE) leaves in Sprague-Dawley rats. Harvested leaves were dried and subjected to solvent extraction using petroleum ether, chloroform, ethyl acetate and methanol in succession. The antioxidant activity of each extract was determined using the ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl dihydrazyl (DPPH) radical scavenging activity. The total phenolic content (TPC) and total flavonoids content (TFC) were estimated by Folin-Ciocalteu and ethanolic aluminium chloride method, respectively. The chloroform extract was found to be highest in flavonoid content, while the methanolic extract showed the highest TPC and antioxidant activity. There was no mortality in rats treated with MECE leaves even at a high dose of 5000 mg/kg body weight. However, the MECE leaves produced mild to moderate pathological changes in the liver and kidneys, shown by mild degenerative changes and leucocyte infiltration. The extract did not affect the haematological parameters or relative weights of the liver or kidneys. Overall, the MECE leaves have potent antioxidant activity and are presumed safe to be used orally as health-promoting product at low to moderate doses.
format Article
author Albaayit, Shaymaa Fadhel Abbas
Abba, Yusuf
Abdullah, Rasedee
Abdullah, Noorlidah
spellingShingle Albaayit, Shaymaa Fadhel Abbas
Abba, Yusuf
Abdullah, Rasedee
Abdullah, Noorlidah
Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract
author_facet Albaayit, Shaymaa Fadhel Abbas
Abba, Yusuf
Abdullah, Rasedee
Abdullah, Noorlidah
author_sort Albaayit, Shaymaa Fadhel Abbas
title Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract
title_short Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract
title_full Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract
title_fullStr Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract
title_full_unstemmed Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract
title_sort evaluation of antioxidant activity and acute toxicity of clausena excavata leaves extract
publisher Hindawi Publishing Corporation
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/37520/1/37520.pdf
http://psasir.upm.edu.my/id/eprint/37520/
http://www.hindawi.com/journals/ecam/2014/975450/abs/
_version_ 1643832004841570304
score 13.211869

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