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Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp

Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsu...

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Main Authors: Tan, Lai Kuan, Ooi, Peck Toung, Carniel, Elisabeth, Thong, Kwai Lin
Format: Article
Language:English
Published: Public Library of Science 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37517/1/37517.pdf
http://psasir.upm.edu.my/id/eprint/37517/
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spelling my.upm.eprints.375172015-12-18T02:48:07Z http://psasir.upm.edu.my/id/eprint/37517/ Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp Tan, Lai Kuan Ooi, Peck Toung Carniel, Elisabeth Thong, Kwai Lin Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsulodin-Irgasan-Novobiocin (CIN) agar in the differentiation of Y. enterocolitica from non-Yersinia species, particularly the natural intestinal microbiota. The modified CIN enabled the growth of Y. enterocolitica colonies with the same efficiency as CIN and Luria-Bertani agar. The detection limits of the modified CIN for Y. enterocolitica in culture medium (10 cfu/ml) and in artificially contaminated pork (104 cfu/ml) were also comparable to those of CIN. However, the modified CIN provided a better discrimination of Yersinia colonies from other bacteria exhibiting Yersinia-like colonies on CIN (H2S-producing Citrobacter freundii, C. braakii, Enterobacter cloacae, Aeromonas hydrophila, Providencia rettgeri, and Morganella morganii). The modified CIN exhibited a higher recovery rate of Y. enterocolitica from artificially prepared bacterial cultures and naturally contaminated samples compared with CIN. Our results thus demonstrated that the use of modified CIN may be a valuable means to increase the recovery rate of food borne Yersinia from natural samples, which are usually contaminated by multiple types of bacteria. Public Library of Science 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37517/1/37517.pdf Tan, Lai Kuan and Ooi, Peck Toung and Carniel, Elisabeth and Thong, Kwai Lin (2014) Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp. PLOS ONE, 9 (8). art. no. e106329. pp. 1-10. ISSN 1932-6203 10.1371/journal.pone.0106329
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsulodin-Irgasan-Novobiocin (CIN) agar in the differentiation of Y. enterocolitica from non-Yersinia species, particularly the natural intestinal microbiota. The modified CIN enabled the growth of Y. enterocolitica colonies with the same efficiency as CIN and Luria-Bertani agar. The detection limits of the modified CIN for Y. enterocolitica in culture medium (10 cfu/ml) and in artificially contaminated pork (104 cfu/ml) were also comparable to those of CIN. However, the modified CIN provided a better discrimination of Yersinia colonies from other bacteria exhibiting Yersinia-like colonies on CIN (H2S-producing Citrobacter freundii, C. braakii, Enterobacter cloacae, Aeromonas hydrophila, Providencia rettgeri, and Morganella morganii). The modified CIN exhibited a higher recovery rate of Y. enterocolitica from artificially prepared bacterial cultures and naturally contaminated samples compared with CIN. Our results thus demonstrated that the use of modified CIN may be a valuable means to increase the recovery rate of food borne Yersinia from natural samples, which are usually contaminated by multiple types of bacteria.
format Article
author Tan, Lai Kuan
Ooi, Peck Toung
Carniel, Elisabeth
Thong, Kwai Lin
spellingShingle Tan, Lai Kuan
Ooi, Peck Toung
Carniel, Elisabeth
Thong, Kwai Lin
Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp
author_facet Tan, Lai Kuan
Ooi, Peck Toung
Carniel, Elisabeth
Thong, Kwai Lin
author_sort Tan, Lai Kuan
title Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp
title_short Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp
title_full Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp
title_fullStr Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp
title_full_unstemmed Evaluation of a modified Cefsulodin-Irgasan-Novobiocin agar for isolation of Yersinia spp
title_sort evaluation of a modified cefsulodin-irgasan-novobiocin agar for isolation of yersinia spp
publisher Public Library of Science
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/37517/1/37517.pdf
http://psasir.upm.edu.my/id/eprint/37517/
_version_ 1643832003954475008
score 13.211869

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