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Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal

Withania somnifera (L) Dunal, commonly known as ashwagandha or Indian ginseng, is the source of large number of pharmacologically active withanolides. Withaferin-A (WS-3), a major withanolide of W. somnifera, has been proven to be an effective anti-cancer molecule. In this study, a liquid culture sy...

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Main Authors: Mir, Bilal Ahmad, Khazir, Jabeena, Hakeem, Khalid Rehman, Koul, Sushma, Cowan, Don A.
Format: Article
Language:English
Published: Springer 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37460/1/Enhanced%20production%20of%20withaferin.pdf
http://psasir.upm.edu.my/id/eprint/37460/
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spelling my.upm.eprints.374602015-10-06T08:52:16Z http://psasir.upm.edu.my/id/eprint/37460/ Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal Mir, Bilal Ahmad Khazir, Jabeena Hakeem, Khalid Rehman Koul, Sushma Cowan, Don A. Withania somnifera (L) Dunal, commonly known as ashwagandha or Indian ginseng, is the source of large number of pharmacologically active withanolides. Withaferin-A (WS-3), a major withanolide of W. somnifera, has been proven to be an effective anti-cancer molecule. In this study, a liquid culture system for shoot proliferation, biomass accumulation and withaferin-A production of an elite accession (AGB002) of W. somnifera was investigated. The nodal explants cultured on Murashige and Skoog (MS) semi-solid medium supplemented with various concentrations of 6-benzyl adenine (BA) and Kinetin (Kn) elicited varied responses. The highest number of regenerated shoots per ex-plant (35 ± 3.25) and the maximum average shoot length (5.0 ± 0.25 cm) were recorded on MS medium supplemented with BA (5.0 μM). The shoots were further proliferated in half and full strength MS liquid medium supplemented with the same concentration BA. It was interesting to note that shoots cultured on MS half strength liquid medium fortified with 4 gL-1 FW (fresh weight) shoot inoculum mass derived from 5 week old nodal explants of W. somnifera showed highest accumulation of biomass and withaferin A content in 5 weeks. Withaferin A was produced in relatively high amounts (1.30 % and 1.10 % DW) in shoots cultured in half and full strength MS liquid media respectively as compared to natural field grown plants (0.85 % DW). A considerable amount of the withaferin A was also excreted in the culture medium. Successful proliferation of shoots in liquid medium and the synthesis of withaferin A in vitro opens new avenues for bioreactor scale-up and the large-scale production of the compound. Springer 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37460/1/Enhanced%20production%20of%20withaferin.pdf Mir, Bilal Ahmad and Khazir, Jabeena and Hakeem, Khalid Rehman and Koul, Sushma and Cowan, Don A. (2014) Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal. Journal of Plant Biochemistry and Biotechnology, 23 (4). pp. 430-434. ISSN 0971-7811; ESSN: 0974-1275 10.1007/s13562-014-0264-8
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Withania somnifera (L) Dunal, commonly known as ashwagandha or Indian ginseng, is the source of large number of pharmacologically active withanolides. Withaferin-A (WS-3), a major withanolide of W. somnifera, has been proven to be an effective anti-cancer molecule. In this study, a liquid culture system for shoot proliferation, biomass accumulation and withaferin-A production of an elite accession (AGB002) of W. somnifera was investigated. The nodal explants cultured on Murashige and Skoog (MS) semi-solid medium supplemented with various concentrations of 6-benzyl adenine (BA) and Kinetin (Kn) elicited varied responses. The highest number of regenerated shoots per ex-plant (35 ± 3.25) and the maximum average shoot length (5.0 ± 0.25 cm) were recorded on MS medium supplemented with BA (5.0 μM). The shoots were further proliferated in half and full strength MS liquid medium supplemented with the same concentration BA. It was interesting to note that shoots cultured on MS half strength liquid medium fortified with 4 gL-1 FW (fresh weight) shoot inoculum mass derived from 5 week old nodal explants of W. somnifera showed highest accumulation of biomass and withaferin A content in 5 weeks. Withaferin A was produced in relatively high amounts (1.30 % and 1.10 % DW) in shoots cultured in half and full strength MS liquid media respectively as compared to natural field grown plants (0.85 % DW). A considerable amount of the withaferin A was also excreted in the culture medium. Successful proliferation of shoots in liquid medium and the synthesis of withaferin A in vitro opens new avenues for bioreactor scale-up and the large-scale production of the compound.
format Article
author Mir, Bilal Ahmad
Khazir, Jabeena
Hakeem, Khalid Rehman
Koul, Sushma
Cowan, Don A.
spellingShingle Mir, Bilal Ahmad
Khazir, Jabeena
Hakeem, Khalid Rehman
Koul, Sushma
Cowan, Don A.
Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal
author_facet Mir, Bilal Ahmad
Khazir, Jabeena
Hakeem, Khalid Rehman
Koul, Sushma
Cowan, Don A.
author_sort Mir, Bilal Ahmad
title Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal
title_short Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal
title_full Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal
title_fullStr Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal
title_full_unstemmed Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal
title_sort enhanced production of withaferin-a in shoot cultures of withania somnifera (l) dunal
publisher Springer
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/37460/1/Enhanced%20production%20of%20withaferin.pdf
http://psasir.upm.edu.my/id/eprint/37460/
_version_ 1643831989406531584
score 13.211869

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