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DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa

The herb plant Mitragyna speciosa (MS) is indigenous in swampy region of Southeast Asia and its traditionally used as a medicine for various illnesses. Recent researches suggested that MS has analgesic effect and it has sedative and stimulant effects depended on given dose. The study aimed to invest...

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Main Authors: Ali, Sumia R. Emhemed, Mohd Moklas, Mohamad Aris, Mat Taib, Che Norma
Format: Article
Published: Research Journal of Pharmaceutical, Biological and Chemical Sciences 2014
Online Access:http://psasir.upm.edu.my/id/eprint/37158/
https://www.rjpbcs.com/2014_5.5.html
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spelling my.upm.eprints.371582023-09-05T11:23:55Z http://psasir.upm.edu.my/id/eprint/37158/ DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa Ali, Sumia R. Emhemed Mohd Moklas, Mohamad Aris Mat Taib, Che Norma The herb plant Mitragyna speciosa (MS) is indigenous in swampy region of Southeast Asia and its traditionally used as a medicine for various illnesses. Recent researches suggested that MS has analgesic effect and it has sedative and stimulant effects depended on given dose. The study aimed to investigate the histopathological changes and expression of both C-fos and DREAM that could be caused by MS extract. Dawley's Sprague rats were orally given the extract at three doses (10, 30, 100 mg/kg) for three weeks and restraint for two hours in restrainer device. The histopthological effects were assessed microscopically, and the C-fos and DREAM expression were evaluated via immunoblot. For the histopathological examination there were no significant changes among (testes, and brain), but kidney showed hypereamia at 30 and 100 mg/kg of nonstress and glumerulus shrunked at 30 mg/kg of stress rats, and for liver there was only slight hymeraemia at 30 of stress and 100 mg/kg of nonstress groups. The immunoblot results for proteins expression showed that; C-fos was expressed in prefrontal (PFC) was significantly increased (p<0.05) at dose 10 mg/kg in both stressed and non-stressed, while it decreased in hippocampus at 100 mg/kg in stressed, and did not show changes in non-stressed rats. DREAM expressed deacrese in PFC at 10 mg/kg in stressed animals, while in hippocampus DREAM expression level was raised significantly at 100 mg/kg in both stressed and non-stressed groups. Thus study showed that MS does not cause histopathological changes, but c-Fos and DREAM showed different expression in targeted areas. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2014 Article PeerReviewed Ali, Sumia R. Emhemed and Mohd Moklas, Mohamad Aris and Mat Taib, Che Norma (2014) DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5 (5). pp. 32-41. ISSN 0975-8585 https://www.rjpbcs.com/2014_5.5.html
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The herb plant Mitragyna speciosa (MS) is indigenous in swampy region of Southeast Asia and its traditionally used as a medicine for various illnesses. Recent researches suggested that MS has analgesic effect and it has sedative and stimulant effects depended on given dose. The study aimed to investigate the histopathological changes and expression of both C-fos and DREAM that could be caused by MS extract. Dawley's Sprague rats were orally given the extract at three doses (10, 30, 100 mg/kg) for three weeks and restraint for two hours in restrainer device. The histopthological effects were assessed microscopically, and the C-fos and DREAM expression were evaluated via immunoblot. For the histopathological examination there were no significant changes among (testes, and brain), but kidney showed hypereamia at 30 and 100 mg/kg of nonstress and glumerulus shrunked at 30 mg/kg of stress rats, and for liver there was only slight hymeraemia at 30 of stress and 100 mg/kg of nonstress groups. The immunoblot results for proteins expression showed that; C-fos was expressed in prefrontal (PFC) was significantly increased (p<0.05) at dose 10 mg/kg in both stressed and non-stressed, while it decreased in hippocampus at 100 mg/kg in stressed, and did not show changes in non-stressed rats. DREAM expressed deacrese in PFC at 10 mg/kg in stressed animals, while in hippocampus DREAM expression level was raised significantly at 100 mg/kg in both stressed and non-stressed groups. Thus study showed that MS does not cause histopathological changes, but c-Fos and DREAM showed different expression in targeted areas.
format Article
author Ali, Sumia R. Emhemed
Mohd Moklas, Mohamad Aris
Mat Taib, Che Norma
spellingShingle Ali, Sumia R. Emhemed
Mohd Moklas, Mohamad Aris
Mat Taib, Che Norma
DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa
author_facet Ali, Sumia R. Emhemed
Mohd Moklas, Mohamad Aris
Mat Taib, Che Norma
author_sort Ali, Sumia R. Emhemed
title DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa
title_short DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa
title_full DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa
title_fullStr DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa
title_full_unstemmed DREAM and C-fos proteins expression after treatment with Malaysian Mitragyna speciosa
title_sort dream and c-fos proteins expression after treatment with malaysian mitragyna speciosa
publisher Research Journal of Pharmaceutical, Biological and Chemical Sciences
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/37158/
https://www.rjpbcs.com/2014_5.5.html
_version_ 1778163705994805248
score 13.211869

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