Cover Image

Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch

Direct assay of resistant starch (RS) in food and feed is accomplished by (i) removal of lipid, protein, and digestible starch to obtain insoluble dietary fiber, and (ii) dissolution of the resistant starch in the insoluble fiber followed by its quantification with specific enzymes. Phosphorylated c...

Full description

Saved in:
Bibliographic Details
Main Authors: Shukri, Radhiah, Zhu, Lijia, Seib, Paul A., Maningat, Clodualdo, Shi, Yong Cheng
Format: Article
Language:English
English
Published: Elsevier 2015
Online Access:http://psasir.upm.edu.my/id/eprint/37112/1/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf
http://psasir.upm.edu.my/id/eprint/37112/2/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf
http://psasir.upm.edu.my/id/eprint/37112/
http://www.sciencedirect.com/science/article/pii/S2212619814000539
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.37112
record_format eprints
spelling my.upm.eprints.371122020-04-15T16:52:07Z http://psasir.upm.edu.my/id/eprint/37112/ Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch Shukri, Radhiah Zhu, Lijia Seib, Paul A. Maningat, Clodualdo Shi, Yong Cheng Direct assay of resistant starch (RS) in food and feed is accomplished by (i) removal of lipid, protein, and digestible starch to obtain insoluble dietary fiber, and (ii) dissolution of the resistant starch in the insoluble fiber followed by its quantification with specific enzymes. Phosphorylated cross-linked (CL) RS resists dissolution and therefore has not been assayed directly. The objective of this study was to develop a method to solubilize the RS fraction in phosphorylated (0.4% phosphorus) CL wheat starch (RS4) after its incubation with α-amylase and amyloglucosidase for 16 h at 37 °C as directed by the RS assay AOAC Method 2002.02. The residue was hydrolyzed and solubilized by conducting two back-to-back incubations with thermostable α-amylase for 30 min at 100 °C and pH 5.0, cooling to 50 °C, then incubating quickly with amyloglucosidase at 50 °C for 1 h at pH 5.0. Importantly, the cooling process after α-amylase incubation was done by placing the mixture in a water bath at 50 °C. The degree of hydrolysis of the CL phosphorylated wheat starch was determined as d-glucose using high-performance anion-exchange chromatography with pulsed amperometric detection (99.0%), glucose-oxidase/peroxidase (95.3%), and phenol-sulfuric acid determination of total carbohydrate (105.2%). Based on those findings, we propose a direct determination of RS in foods containing phosphorylated CL wheat starch. Elsevier 2015-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37112/1/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/37112/2/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf Shukri, Radhiah and Zhu, Lijia and Seib, Paul A. and Maningat, Clodualdo and Shi, Yong Cheng (2015) Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch. Bioactive Carbohydrates and Dietary Fibre, 5 (1). pp. 1-9. ISSN 2212-6198 http://www.sciencedirect.com/science/article/pii/S2212619814000539 10.1016/j.bcdf.2014.11.001
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Direct assay of resistant starch (RS) in food and feed is accomplished by (i) removal of lipid, protein, and digestible starch to obtain insoluble dietary fiber, and (ii) dissolution of the resistant starch in the insoluble fiber followed by its quantification with specific enzymes. Phosphorylated cross-linked (CL) RS resists dissolution and therefore has not been assayed directly. The objective of this study was to develop a method to solubilize the RS fraction in phosphorylated (0.4% phosphorus) CL wheat starch (RS4) after its incubation with α-amylase and amyloglucosidase for 16 h at 37 °C as directed by the RS assay AOAC Method 2002.02. The residue was hydrolyzed and solubilized by conducting two back-to-back incubations with thermostable α-amylase for 30 min at 100 °C and pH 5.0, cooling to 50 °C, then incubating quickly with amyloglucosidase at 50 °C for 1 h at pH 5.0. Importantly, the cooling process after α-amylase incubation was done by placing the mixture in a water bath at 50 °C. The degree of hydrolysis of the CL phosphorylated wheat starch was determined as d-glucose using high-performance anion-exchange chromatography with pulsed amperometric detection (99.0%), glucose-oxidase/peroxidase (95.3%), and phenol-sulfuric acid determination of total carbohydrate (105.2%). Based on those findings, we propose a direct determination of RS in foods containing phosphorylated CL wheat starch.
format Article
author Shukri, Radhiah
Zhu, Lijia
Seib, Paul A.
Maningat, Clodualdo
Shi, Yong Cheng
spellingShingle Shukri, Radhiah
Zhu, Lijia
Seib, Paul A.
Maningat, Clodualdo
Shi, Yong Cheng
Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
author_facet Shukri, Radhiah
Zhu, Lijia
Seib, Paul A.
Maningat, Clodualdo
Shi, Yong Cheng
author_sort Shukri, Radhiah
title Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
title_short Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
title_full Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
title_fullStr Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
title_full_unstemmed Direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
title_sort direct in-vitro assay of resistant starch in phosphorylated cross-linked starch
publisher Elsevier
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/37112/1/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf
http://psasir.upm.edu.my/id/eprint/37112/2/Direct%20in-vitro%20assay%20of%20resistant%20starch%20in%20phosphorylated%20cross-linked%20starch.pdf
http://psasir.upm.edu.my/id/eprint/37112/
http://www.sciencedirect.com/science/article/pii/S2212619814000539
_version_ 1665895948576882688
score 13.236483

Similar Items