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Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia

Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplifi...

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Main Authors: Low, Fish Cheng Yee, Hamzah, Ainon, Chee, Hui Yee
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:http://psasir.upm.edu.my/id/eprint/36946/1/Culture-independent-based%20analyses%20identified%20site-specific%20microbial%20in%20two%20oil-wells%20from%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/36946/
http://www.ukm.my/jsm/english_journals/vol44num11_2015/contentsVol44num11_2015.html
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spelling my.upm.eprints.369462020-07-06T03:33:30Z http://psasir.upm.edu.my/id/eprint/36946/ Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia Low, Fish Cheng Yee Hamzah, Ainon Chee, Hui Yee Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplified products were purified and cloned into pTZ57R/T vector to construct the 16S/18S rDNA library. Restriction endocucleases HhaI and MspI were used to screen the library. From that, 125 and 253 recombinant plasmid representative clones from Platform Bo and Platform Pe, respectively, were sent for DNA sequencing. Twenty-six operational taxonomic units (OTUs) consist of 20 genera detected at Platform Bo and 17 OTUs consist of 13 genera detected at Platform Pe. Marinobacter and Acinetobacter species co-occurred in both platforms whereas the rest are site-specific. Gammaproteobacteria accounted for 86.0% of the microbial community in Platform Bo, where OTUs affiliated to Marinobacter, Pseudomonas and Marinobacterium that were the most abundant. The major OTUs in the Platform Pe were with affinities to Achromobacter, followed by Stenotrophomonas and Serratia. The only archaeal isolates were detected in Platform Pe, which affiliated to Thermocladium. The singletons and doubletons accounted for about 50.0% of the OTU abundance in both platforms, which considerably significant despite their rare occurrence. Penerbit Universiti Kebangsaan Malaysia 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/36946/1/Culture-independent-based%20analyses%20identified%20site-specific%20microbial%20in%20two%20oil-wells%20from%20Malaysia.pdf Low, Fish Cheng Yee and Hamzah, Ainon and Chee, Hui Yee (2015) Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia. Sains Malaysiana, 44 (11). pp. 1625-1633. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol44num11_2015/contentsVol44num11_2015.html
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplified products were purified and cloned into pTZ57R/T vector to construct the 16S/18S rDNA library. Restriction endocucleases HhaI and MspI were used to screen the library. From that, 125 and 253 recombinant plasmid representative clones from Platform Bo and Platform Pe, respectively, were sent for DNA sequencing. Twenty-six operational taxonomic units (OTUs) consist of 20 genera detected at Platform Bo and 17 OTUs consist of 13 genera detected at Platform Pe. Marinobacter and Acinetobacter species co-occurred in both platforms whereas the rest are site-specific. Gammaproteobacteria accounted for 86.0% of the microbial community in Platform Bo, where OTUs affiliated to Marinobacter, Pseudomonas and Marinobacterium that were the most abundant. The major OTUs in the Platform Pe were with affinities to Achromobacter, followed by Stenotrophomonas and Serratia. The only archaeal isolates were detected in Platform Pe, which affiliated to Thermocladium. The singletons and doubletons accounted for about 50.0% of the OTU abundance in both platforms, which considerably significant despite their rare occurrence.
format Article
author Low, Fish Cheng Yee
Hamzah, Ainon
Chee, Hui Yee
spellingShingle Low, Fish Cheng Yee
Hamzah, Ainon
Chee, Hui Yee
Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
author_facet Low, Fish Cheng Yee
Hamzah, Ainon
Chee, Hui Yee
author_sort Low, Fish Cheng Yee
title Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_short Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_full Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_fullStr Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_full_unstemmed Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_sort culture-independent-based analyses identified site-specific microbial in two oil-wells from malaysia
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/36946/1/Culture-independent-based%20analyses%20identified%20site-specific%20microbial%20in%20two%20oil-wells%20from%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/36946/
http://www.ukm.my/jsm/english_journals/vol44num11_2015/contentsVol44num11_2015.html
_version_ 1672612187031470080
score 13.211869

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