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Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum

Lactobacillus plantarum PA18, a strain originally isolated from the leaves of Pandanus amaryllifolius, contains a pR18 plasmid. The pR18 plasmid is a 3211 bp circular molecule with a G + C content of 35.8%. Nucleotide sequence analysis revealed two putative open reading frames, ORF1 and ORF2, in whi...

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Main Authors: Jalilsood, Tannaz, Baradaran, Ali, Foo, Hooi Ling, Mustafa, Shuhaimi, Yusoff, Khatijah, Abdul Rahim, Raha
Format: Article
Language:English
Published: Elsevier 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36762/1/Characterization%20of%20pR18.pdf
http://psasir.upm.edu.my/id/eprint/36762/
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spelling my.upm.eprints.367622015-10-05T00:54:01Z http://psasir.upm.edu.my/id/eprint/36762/ Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum Jalilsood, Tannaz Baradaran, Ali Foo, Hooi Ling Mustafa, Shuhaimi Yusoff, Khatijah Abdul Rahim, Raha Lactobacillus plantarum PA18, a strain originally isolated from the leaves of Pandanus amaryllifolius, contains a pR18 plasmid. The pR18 plasmid is a 3211 bp circular molecule with a G + C content of 35.8%. Nucleotide sequence analysis revealed two putative open reading frames, ORF1 and ORF2, in which ORF2 was predicted (317 amino acids) to be a replication protein and shared 99% similarity with the Rep proteins of pLR1, pLD1, pC30il, and pLP2000, which belong to the RCR pC194/pUB110 family. Sequence analysis also indicated that ORF1 was predicted to encode linA, an enzyme that enzymatically inactivates lincomycin. The result of Southern hybridization and mung bean nuclease treatment confirmed that pR18 replicated via the RCR mechanism. Phylogenetic tree analysis of pR18 plasmid proteins suggested that horizontal transfer of antibiotic resistance determinants without genes encoding mobilization has not only occurred between Bacillus and Lactobacillus but also between unrelated bacteria. Understanding this type of transfer could possibly play a key role in facilitating the study of the origin and evolution of lactobacillus plasmids. Quantitative PCR showed that the relative copy number of pR18 was approximately 39 copies per chromosome equivalent. Elsevier 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36762/1/Characterization%20of%20pR18.pdf Jalilsood, Tannaz and Baradaran, Ali and Foo, Hooi Ling and Mustafa, Shuhaimi and Yusoff, Khatijah and Abdul Rahim, Raha (2014) Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum. Plasmid, 73. pp. 1-9. ISSN 0147-619X; ESSN:1095-9890 10.1016/j.plasmid.2014.04.004
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Lactobacillus plantarum PA18, a strain originally isolated from the leaves of Pandanus amaryllifolius, contains a pR18 plasmid. The pR18 plasmid is a 3211 bp circular molecule with a G + C content of 35.8%. Nucleotide sequence analysis revealed two putative open reading frames, ORF1 and ORF2, in which ORF2 was predicted (317 amino acids) to be a replication protein and shared 99% similarity with the Rep proteins of pLR1, pLD1, pC30il, and pLP2000, which belong to the RCR pC194/pUB110 family. Sequence analysis also indicated that ORF1 was predicted to encode linA, an enzyme that enzymatically inactivates lincomycin. The result of Southern hybridization and mung bean nuclease treatment confirmed that pR18 replicated via the RCR mechanism. Phylogenetic tree analysis of pR18 plasmid proteins suggested that horizontal transfer of antibiotic resistance determinants without genes encoding mobilization has not only occurred between Bacillus and Lactobacillus but also between unrelated bacteria. Understanding this type of transfer could possibly play a key role in facilitating the study of the origin and evolution of lactobacillus plasmids. Quantitative PCR showed that the relative copy number of pR18 was approximately 39 copies per chromosome equivalent.
format Article
author Jalilsood, Tannaz
Baradaran, Ali
Foo, Hooi Ling
Mustafa, Shuhaimi
Yusoff, Khatijah
Abdul Rahim, Raha
spellingShingle Jalilsood, Tannaz
Baradaran, Ali
Foo, Hooi Ling
Mustafa, Shuhaimi
Yusoff, Khatijah
Abdul Rahim, Raha
Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum
author_facet Jalilsood, Tannaz
Baradaran, Ali
Foo, Hooi Ling
Mustafa, Shuhaimi
Yusoff, Khatijah
Abdul Rahim, Raha
author_sort Jalilsood, Tannaz
title Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum
title_short Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum
title_full Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum
title_fullStr Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum
title_full_unstemmed Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum
title_sort characterization of pr18, a novel rolling-circle replication plasmid from lactobacillus plantarum
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36762/1/Characterization%20of%20pR18.pdf
http://psasir.upm.edu.my/id/eprint/36762/
_version_ 1643831824474963968
score 13.211869

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