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Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication

Surra is a hemoprotozoal disease affecting domesticated and wildlife animals. The causative agent is the hemoprotozoan parasite Trypanosoma evansi. Only few drugs are currently available for the treatment of this disease that are old and encounter the problem of resistance emergence, which punctuate...

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Main Authors: Dyary, Hiewa O., Abdul Kadir, Arifah, Sharma, Reuben Sunil Kumar, Abdullah, Rasedee
Format: Article
Language:English
Published: Faculty of Veterinary Science, University of Agriculture, Faisalabad 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36499/1/Antitrypanosomal%20and%20cytotoxic%20activities%20of%20selected%20medicinal%20plants%20and%20effect%20of%20Cordyline%20terminalis%20on%20trypanosomal%20nuclear%20and%20kinetoplast%20replication.pdf
http://psasir.upm.edu.my/id/eprint/36499/
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spelling my.upm.eprints.364992015-10-06T03:20:01Z http://psasir.upm.edu.my/id/eprint/36499/ Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication Dyary, Hiewa O. Abdul Kadir, Arifah Sharma, Reuben Sunil Kumar Abdullah, Rasedee Surra is a hemoprotozoal disease affecting domesticated and wildlife animals. The causative agent is the hemoprotozoan parasite Trypanosoma evansi. Only few drugs are currently available for the treatment of this disease that are old and encounter the problem of resistance emergence, which punctuates the urgent demand for new drugs for the treatment of surra. In the current study, the antitrypanosomal activity of the aqueous and ethanol extracts of five selected medicinal plants namely Acanthus ilicifolius, Allium sativum, Cordyline terminalis, Goniothalamus tapis and Maesa ramentacea was evaluated in vitro against T. evansi strain Te7 and the cytotoxic activity of the extracts was evaluated on Vero cells using MTT-cell proliferation assay. The ethanol extract of leaves of G. tapis scored the most potent antitrypanosomal activity (IC50 of 7.61μg/ml) and a selectivity index (SI) of 11.47, while the aqueous extract of C. terminalis leaves exhibited an IC50 of 48.1 μg/ml and the highest SI of 27.21. Addition of 50 μg/ml of C. terminalis extract to T. evansi culture has led to a significant inhibition of nuclear and kinetoplast DNA replication, contributing to its in vitro antitrypanosomal activity. From the current study, it can be concluded that the aqueous extract of leaves of C. terminalis shows considerable antitrypanosomal activity and it could be a potential source of new antitrypanosomal compounds. Faculty of Veterinary Science, University of Agriculture, Faisalabad 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36499/1/Antitrypanosomal%20and%20cytotoxic%20activities%20of%20selected%20medicinal%20plants%20and%20effect%20of%20Cordyline%20terminalis%20on%20trypanosomal%20nuclear%20and%20kinetoplast%20replication.pdf Dyary, Hiewa O. and Abdul Kadir, Arifah and Sharma, Reuben Sunil Kumar and Abdullah, Rasedee (2014) Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication. Pakistan Veterinary Journal, 34 (4). pp. 444-448. ISSN 0253-8318; ESSN: 2074-7764
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Surra is a hemoprotozoal disease affecting domesticated and wildlife animals. The causative agent is the hemoprotozoan parasite Trypanosoma evansi. Only few drugs are currently available for the treatment of this disease that are old and encounter the problem of resistance emergence, which punctuates the urgent demand for new drugs for the treatment of surra. In the current study, the antitrypanosomal activity of the aqueous and ethanol extracts of five selected medicinal plants namely Acanthus ilicifolius, Allium sativum, Cordyline terminalis, Goniothalamus tapis and Maesa ramentacea was evaluated in vitro against T. evansi strain Te7 and the cytotoxic activity of the extracts was evaluated on Vero cells using MTT-cell proliferation assay. The ethanol extract of leaves of G. tapis scored the most potent antitrypanosomal activity (IC50 of 7.61μg/ml) and a selectivity index (SI) of 11.47, while the aqueous extract of C. terminalis leaves exhibited an IC50 of 48.1 μg/ml and the highest SI of 27.21. Addition of 50 μg/ml of C. terminalis extract to T. evansi culture has led to a significant inhibition of nuclear and kinetoplast DNA replication, contributing to its in vitro antitrypanosomal activity. From the current study, it can be concluded that the aqueous extract of leaves of C. terminalis shows considerable antitrypanosomal activity and it could be a potential source of new antitrypanosomal compounds.
format Article
author Dyary, Hiewa O.
Abdul Kadir, Arifah
Sharma, Reuben Sunil Kumar
Abdullah, Rasedee
spellingShingle Dyary, Hiewa O.
Abdul Kadir, Arifah
Sharma, Reuben Sunil Kumar
Abdullah, Rasedee
Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication
author_facet Dyary, Hiewa O.
Abdul Kadir, Arifah
Sharma, Reuben Sunil Kumar
Abdullah, Rasedee
author_sort Dyary, Hiewa O.
title Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication
title_short Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication
title_full Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication
title_fullStr Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication
title_full_unstemmed Antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of Cordyline terminalis on trypanosomal nuclear and kinetoplast replication
title_sort antitrypanosomal and cytotoxic activities of selected medicinal plants and effect of cordyline terminalis on trypanosomal nuclear and kinetoplast replication
publisher Faculty of Veterinary Science, University of Agriculture, Faisalabad
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36499/1/Antitrypanosomal%20and%20cytotoxic%20activities%20of%20selected%20medicinal%20plants%20and%20effect%20of%20Cordyline%20terminalis%20on%20trypanosomal%20nuclear%20and%20kinetoplast%20replication.pdf
http://psasir.upm.edu.my/id/eprint/36499/
_version_ 1643831756278726656
score 13.211869

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