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Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients

A total of 103 group B streptococci (GBS) including 22 invasive, 21 non-invasive, and 60 colonizing isolates were collected in a Malaysian hospital (June 2010–October 2011). Isolates were characterized by conventional and molecular serotyping and analyzed for scpB, lmb, hylB, cylE, bac, bca and rib...

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Main Authors: Eskandarian, N., Ismail, Zalina, Neela, Vasantha Kumari, Belkum, Alex van, Mohd Desa, Mohd Nasir, Amin Nordin, Syafinaz
Format: Article
Language:English
Published: Springer 2015
Online Access:http://psasir.upm.edu.my/id/eprint/36485/1/Antimicrobial%20susceptibility%20profiles.pdf
http://psasir.upm.edu.my/id/eprint/36485/
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spelling my.upm.eprints.364852015-09-08T02:50:36Z http://psasir.upm.edu.my/id/eprint/36485/ Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients Eskandarian, N. Ismail, Zalina Neela, Vasantha Kumari Belkum, Alex van Mohd Desa, Mohd Nasir Amin Nordin, Syafinaz A total of 103 group B streptococci (GBS) including 22 invasive, 21 non-invasive, and 60 colonizing isolates were collected in a Malaysian hospital (June 2010–October 2011). Isolates were characterized by conventional and molecular serotyping and analyzed for scpB, lmb, hylB, cylE, bac, bca and rib gene content. Antimicrobial susceptibility to penicillins, macrolides, lincosamides, quinolones and tetracyclines was determined using disk diffusion and the MICs for penicillin were determined by E-test. Molecular serotyping for all eight serotypes (Ia, Ib, II–VII) was in full accordance with conventional serotyping. Overall, taking CS and MS together, serotype VI was the most common capsular type (22.3 %) followed by VII (21.4 %), III (20.4 %), Ia (17.5 %), V (9.7 %), II (7.7 %) and IV (1 %). Susceptibility to beta-lactam antimicrobials was prevalent (100 %). Resistance rates for erythromycin, clindamycin and tetracycline were 23.3 %, 17.5 % and 71.8 %, respectively. PCR-virulence gene screening showed the presence of cylE, lmb, scpB and hylB in almost all the isolates while rib, bca, and bac genes were found in 29.1 %, 14.6 % and 9.7 % of the isolates. Certain genes were significantly associated with specific serotypes, namely, rib with serotypes Ia, II, III and VI; bca and bac with serotypes II and III. Furthermore, serotype Ia was significantly more common among patients with invasive infections (p < 0.01) and serotype VI isolates were significantly more common among carriers (p < 0.05). In summary, serotype distribution correlates with virulence gene content will be useful in epidemiological studies and design of vaccines. Springer 2015-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36485/1/Antimicrobial%20susceptibility%20profiles.pdf Eskandarian, N. and Ismail, Zalina and Neela, Vasantha Kumari and Belkum, Alex van and Mohd Desa, Mohd Nasir and Amin Nordin, Syafinaz (2015) Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients. European Journal of Clinical Microbiology & Infectious Diseases, 34 (3). pp. 579-584. ISSN 0934-9723; ESSN: 1435-4373 10.1007/s10096-014-2265-x
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A total of 103 group B streptococci (GBS) including 22 invasive, 21 non-invasive, and 60 colonizing isolates were collected in a Malaysian hospital (June 2010–October 2011). Isolates were characterized by conventional and molecular serotyping and analyzed for scpB, lmb, hylB, cylE, bac, bca and rib gene content. Antimicrobial susceptibility to penicillins, macrolides, lincosamides, quinolones and tetracyclines was determined using disk diffusion and the MICs for penicillin were determined by E-test. Molecular serotyping for all eight serotypes (Ia, Ib, II–VII) was in full accordance with conventional serotyping. Overall, taking CS and MS together, serotype VI was the most common capsular type (22.3 %) followed by VII (21.4 %), III (20.4 %), Ia (17.5 %), V (9.7 %), II (7.7 %) and IV (1 %). Susceptibility to beta-lactam antimicrobials was prevalent (100 %). Resistance rates for erythromycin, clindamycin and tetracycline were 23.3 %, 17.5 % and 71.8 %, respectively. PCR-virulence gene screening showed the presence of cylE, lmb, scpB and hylB in almost all the isolates while rib, bca, and bac genes were found in 29.1 %, 14.6 % and 9.7 % of the isolates. Certain genes were significantly associated with specific serotypes, namely, rib with serotypes Ia, II, III and VI; bca and bac with serotypes II and III. Furthermore, serotype Ia was significantly more common among patients with invasive infections (p < 0.01) and serotype VI isolates were significantly more common among carriers (p < 0.05). In summary, serotype distribution correlates with virulence gene content will be useful in epidemiological studies and design of vaccines.
format Article
author Eskandarian, N.
Ismail, Zalina
Neela, Vasantha Kumari
Belkum, Alex van
Mohd Desa, Mohd Nasir
Amin Nordin, Syafinaz
spellingShingle Eskandarian, N.
Ismail, Zalina
Neela, Vasantha Kumari
Belkum, Alex van
Mohd Desa, Mohd Nasir
Amin Nordin, Syafinaz
Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients
author_facet Eskandarian, N.
Ismail, Zalina
Neela, Vasantha Kumari
Belkum, Alex van
Mohd Desa, Mohd Nasir
Amin Nordin, Syafinaz
author_sort Eskandarian, N.
title Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients
title_short Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients
title_full Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients
title_fullStr Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients
title_full_unstemmed Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients
title_sort antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing streptococcus agalactiae (group b streptococcus) from malaysian patients
publisher Springer
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/36485/1/Antimicrobial%20susceptibility%20profiles.pdf
http://psasir.upm.edu.my/id/eprint/36485/
_version_ 1643831752095956992
score 13.211869

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