Cover Image

Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease

Type 2 diabetes mellitus (T2DM) is believed to be associated with excessive production of reactive oxygen species. Glutathione S-transferase (GST) polymorphisms result in decreased or absent enzyme activity and altered oxidative stress, and have been associated with cardiovascular disease (CVD). The...

Full description

Saved in:
Bibliographic Details
Main Authors: Etemad, Ali, Ramachandran, Vasudevan, Abdul Aziz, Ahmad Fazli, Mohamed Yusof, Ahmad Khairuddin, Khazaei, Somayeh, Fawzi, Nora, Jamalpour, Sajad, Arkani, Mohammad, Mohamad, Nur Afiqah, Ismail, Patimah
Format: Article
Language:English
Published: Research Foundation of Ribeirão Preto 2016
Online Access:http://psasir.upm.edu.my/id/eprint/36451/1/Analysis%20of%20selected%20glutathione%20S-transferase%20gene%20polymorphisms%20in%20Malaysian%20type%202%20diabetes%20mellitus%20patients%20with%20and%20without%20cardiovascular%20disease.pdf
http://psasir.upm.edu.my/id/eprint/36451/
https://www.geneticsmr.com/articles/6240
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.36451
record_format eprints
spelling my.upm.eprints.364512020-07-06T03:06:32Z http://psasir.upm.edu.my/id/eprint/36451/ Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease Etemad, Ali Ramachandran, Vasudevan Abdul Aziz, Ahmad Fazli Mohamed Yusof, Ahmad Khairuddin Khazaei, Somayeh Fawzi, Nora Jamalpour, Sajad Arkani, Mohammad Mohamad, Nur Afiqah Ismail, Patimah Type 2 diabetes mellitus (T2DM) is believed to be associated with excessive production of reactive oxygen species. Glutathione S-transferase (GST) polymorphisms result in decreased or absent enzyme activity and altered oxidative stress, and have been associated with cardiovascular disease (CVD). The present study assessed the effect of GST polymorphisms on the risk of developing T2DM in individuals of Malaysian Malay ethnicity. A total of 287 subjects, consisting of 87 T2DM and 64 CVD/T2DM patients, as well as 136 healthy gender- and age-matched controls were genotyped for selected polymorphisms to evaluate associations with T2DM susceptibility. Genomic DNA was extracted using commercially available kits, and GSTM1, GSTT1, and α-globin sequences were amplified by multiplex polymerase chain reaction. Biochemical parameters were measured with a Hitachi autoanalyzer. The Fisher exact test, the chi-square statistic, and means ± standard deviations were calculated using the SPSS software. Overall, we observed no significant differences regarding genotype and allele frequencies between each group (P = 0.224 and 0.199, respectively). However, in the combined analysis of genotypes and blood measurements, fasting plasma glucose, HbA1c, and triglyceride levels, followed by age, body mass index, waist-hip ratio, systolic blood pressure, and history of T2DM significantly differed according to GST polymorphism (P ˂ 0.05). Genetically induced absence of the GSTT1 enzyme is an independent and powerful predictor of premature vascular morbidity and death in individuals with T2DM, and might be triggered by cigarette smoking’s oxidative effects. These polymorphisms could be screened in other ethnicities within Malaysia to determine further possible risk factors. Research Foundation of Ribeirão Preto 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/36451/1/Analysis%20of%20selected%20glutathione%20S-transferase%20gene%20polymorphisms%20in%20Malaysian%20type%202%20diabetes%20mellitus%20patients%20with%20and%20without%20cardiovascular%20disease.pdf Etemad, Ali and Ramachandran, Vasudevan and Abdul Aziz, Ahmad Fazli and Mohamed Yusof, Ahmad Khairuddin and Khazaei, Somayeh and Fawzi, Nora and Jamalpour, Sajad and Arkani, Mohammad and Mohamad, Nur Afiqah and Ismail, Patimah (2016) Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease. Genetics and Molecular Research, 15 (2). art. no. gmr.15025845. pp. 1-9. ISSN 1676-5680 https://www.geneticsmr.com/articles/6240 10.4238/gmr.15025845
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Type 2 diabetes mellitus (T2DM) is believed to be associated with excessive production of reactive oxygen species. Glutathione S-transferase (GST) polymorphisms result in decreased or absent enzyme activity and altered oxidative stress, and have been associated with cardiovascular disease (CVD). The present study assessed the effect of GST polymorphisms on the risk of developing T2DM in individuals of Malaysian Malay ethnicity. A total of 287 subjects, consisting of 87 T2DM and 64 CVD/T2DM patients, as well as 136 healthy gender- and age-matched controls were genotyped for selected polymorphisms to evaluate associations with T2DM susceptibility. Genomic DNA was extracted using commercially available kits, and GSTM1, GSTT1, and α-globin sequences were amplified by multiplex polymerase chain reaction. Biochemical parameters were measured with a Hitachi autoanalyzer. The Fisher exact test, the chi-square statistic, and means ± standard deviations were calculated using the SPSS software. Overall, we observed no significant differences regarding genotype and allele frequencies between each group (P = 0.224 and 0.199, respectively). However, in the combined analysis of genotypes and blood measurements, fasting plasma glucose, HbA1c, and triglyceride levels, followed by age, body mass index, waist-hip ratio, systolic blood pressure, and history of T2DM significantly differed according to GST polymorphism (P ˂ 0.05). Genetically induced absence of the GSTT1 enzyme is an independent and powerful predictor of premature vascular morbidity and death in individuals with T2DM, and might be triggered by cigarette smoking’s oxidative effects. These polymorphisms could be screened in other ethnicities within Malaysia to determine further possible risk factors.
format Article
author Etemad, Ali
Ramachandran, Vasudevan
Abdul Aziz, Ahmad Fazli
Mohamed Yusof, Ahmad Khairuddin
Khazaei, Somayeh
Fawzi, Nora
Jamalpour, Sajad
Arkani, Mohammad
Mohamad, Nur Afiqah
Ismail, Patimah
spellingShingle Etemad, Ali
Ramachandran, Vasudevan
Abdul Aziz, Ahmad Fazli
Mohamed Yusof, Ahmad Khairuddin
Khazaei, Somayeh
Fawzi, Nora
Jamalpour, Sajad
Arkani, Mohammad
Mohamad, Nur Afiqah
Ismail, Patimah
Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
author_facet Etemad, Ali
Ramachandran, Vasudevan
Abdul Aziz, Ahmad Fazli
Mohamed Yusof, Ahmad Khairuddin
Khazaei, Somayeh
Fawzi, Nora
Jamalpour, Sajad
Arkani, Mohammad
Mohamad, Nur Afiqah
Ismail, Patimah
author_sort Etemad, Ali
title Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
title_short Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
title_full Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
title_fullStr Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
title_full_unstemmed Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
title_sort analysis of selected glutathione s-transferase gene polymorphisms in malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
publisher Research Foundation of Ribeirão Preto
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/36451/1/Analysis%20of%20selected%20glutathione%20S-transferase%20gene%20polymorphisms%20in%20Malaysian%20type%202%20diabetes%20mellitus%20patients%20with%20and%20without%20cardiovascular%20disease.pdf
http://psasir.upm.edu.my/id/eprint/36451/
https://www.geneticsmr.com/articles/6240
_version_ 1672612183908810752
score 13.211869

Similar Items