Staff View: Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen

Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen

Vitamin C is generally thought to enhance immunity and is widely taken as a supplement especially during cancer treatment. Tamoxifen (TAM) has both cytostatic and cytotoxic properties for breast cancer. TAM engaged mitochondrial oestrogen receptor beta in MCF-7 cells and induces apoptosis by activat...

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Main Authors:	Subramani, Tamilselvan, Yeap, Swee Keong, Ho, Wan Yang, Ho, Chai Ling, Omar, Abdul Rahman, Abdul Aziz, Suraini, Nik Abd. Rahman, Nik Mohd. Afizan, Mohamed Alitheen, Noorjahan Banu
Format:	Article
Published:	Wiley-Blackwell Publishing 2014
Online Access:	http://psasir.upm.edu.my/id/eprint/35992/ http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12188/abstract
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spelling	my.upm.eprints.359922016-02-12T07:21:54Z http://psasir.upm.edu.my/id/eprint/35992/ Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yang Ho, Chai Ling Omar, Abdul Rahman Abdul Aziz, Suraini Nik Abd. Rahman, Nik Mohd. Afizan Mohamed Alitheen, Noorjahan Banu Vitamin C is generally thought to enhance immunity and is widely taken as a supplement especially during cancer treatment. Tamoxifen (TAM) has both cytostatic and cytotoxic properties for breast cancer. TAM engaged mitochondrial oestrogen receptor beta in MCF-7 cells and induces apoptosis by activation of pro-caspase-8 followed by downstream events, including an increase in reactive oxygen species and the release of pro-apoptotic factors from the mitochondria. In addition to that, TAM binds with high affinity to the microsomal anti-oestrogen-binding site and inhibits cholesterol esterification at therapeutic doses. This study aimed to investigate the role of vitamin C in TAM-mediated apoptosis. Cells were loaded with vitamin C by exposure to dehydroascorbic acid, thereby circumventing in vitro artefacts associated with the poor transport and pro-oxidant effects of ascorbic acid. Pre-treatment with vitamin C caused a dose-dependent attenuation of cytotoxicity, as measured by acridine-orange/propidium iodide (AO/PI) and Annexin V assay after treatment with TAM. Vitamin C dose-dependently protected cancer cells against lipid peroxidation caused by TAM treatment. By real-time PCR analysis, an impressive increase in FasL and tumour necrosis factor-α (TNF-α) mRNA was detected after TAM treatment. In addition, a decrease in mitochondrial transmembrane potential was observed. These results support the hypothesis that vitamin C supplementation during cancer treatment may detrimentally affect therapeutic response. Wiley-Blackwell Publishing 2014-02 Article PeerReviewed Subramani, Tamilselvan and Yeap, Swee Keong and Ho, Wan Yang and Ho, Chai Ling and Omar, Abdul Rahman and Abdul Aziz, Suraini and Nik Abd. Rahman, Nik Mohd. Afizan and Mohamed Alitheen, Noorjahan Banu (2014) Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen. Journal of Cellular and Molecular Medicine, 18 (2). pp. 305-313. ISSN 1582-1838; ESSN: 1582-4934 http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12188/abstract 10.1111/jcmm.12188
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Vitamin C is generally thought to enhance immunity and is widely taken as a supplement especially during cancer treatment. Tamoxifen (TAM) has both cytostatic and cytotoxic properties for breast cancer. TAM engaged mitochondrial oestrogen receptor beta in MCF-7 cells and induces apoptosis by activation of pro-caspase-8 followed by downstream events, including an increase in reactive oxygen species and the release of pro-apoptotic factors from the mitochondria. In addition to that, TAM binds with high affinity to the microsomal anti-oestrogen-binding site and inhibits cholesterol esterification at therapeutic doses. This study aimed to investigate the role of vitamin C in TAM-mediated apoptosis. Cells were loaded with vitamin C by exposure to dehydroascorbic acid, thereby circumventing in vitro artefacts associated with the poor transport and pro-oxidant effects of ascorbic acid. Pre-treatment with vitamin C caused a dose-dependent attenuation of cytotoxicity, as measured by acridine-orange/propidium iodide (AO/PI) and Annexin V assay after treatment with TAM. Vitamin C dose-dependently protected cancer cells against lipid peroxidation caused by TAM treatment. By real-time PCR analysis, an impressive increase in FasL and tumour necrosis factor-α (TNF-α) mRNA was detected after TAM treatment. In addition, a decrease in mitochondrial transmembrane potential was observed. These results support the hypothesis that vitamin C supplementation during cancer treatment may detrimentally affect therapeutic response.
format	Article
author	Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yang Ho, Chai Ling Omar, Abdul Rahman Abdul Aziz, Suraini Nik Abd. Rahman, Nik Mohd. Afizan Mohamed Alitheen, Noorjahan Banu
spellingShingle	Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yang Ho, Chai Ling Omar, Abdul Rahman Abdul Aziz, Suraini Nik Abd. Rahman, Nik Mohd. Afizan Mohamed Alitheen, Noorjahan Banu Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen
author_facet	Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yang Ho, Chai Ling Omar, Abdul Rahman Abdul Aziz, Suraini Nik Abd. Rahman, Nik Mohd. Afizan Mohamed Alitheen, Noorjahan Banu
author_sort	Subramani, Tamilselvan
title	Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen
title_short	Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen
title_full	Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen
title_fullStr	Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen
title_full_unstemmed	Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen
title_sort	vitamin c suppresses cell death in mcf-7 human breast cancer cells induced by tamoxifen
publisher	Wiley-Blackwell Publishing
publishDate	2014
url	http://psasir.upm.edu.my/id/eprint/35992/ http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12188/abstract
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score	13.211869

Vitamin C suppresses cell death in MCF-7 human breast cancer cells induced by tamoxifen

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