Two-phase fed-batch modification for 48 hour peak expression of hepatitis B surface antigen in Pichia pastoris shake flask system
A study of the Mut+ phenotype for the expression of recombinant hepatitis B surface antigen (HBsAg) in Pichia pastoris strain GS115 using the pPIC3.5K vector with a two-phase fed-batch protocol in a shake flask system is described. Expression levels of HBsAg protein of 6.74 g L−1 Dry Cell Weight (DC...
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| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35906/1/Two-phase%20fed-batch%20modification%20for%2048%20hour%20peak%20expression%20of%20hepatitis%20B%20surface%20antigen%20in%20Pichia%20pastoris%20shake%20flask%20system.pdf http://psasir.upm.edu.my/id/eprint/35906/ http://link.springer.com/article/10.2478%2Fs11535-014-0309-y |
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http://psasir.upm.edu.my/id/eprint/35906/1/Two-phase%20fed-batch%20modification%20for%2048%20hour%20peak%20expression%20of%20hepatitis%20B%20surface%20antigen%20in%20Pichia%20pastoris%20shake%20flask%20system.pdfhttp://psasir.upm.edu.my/id/eprint/35906/
http://link.springer.com/article/10.2478%2Fs11535-014-0309-y