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Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption

The purification of serine protease from crude mango peel feedstock using heat-treated expanded bed adsorption was investigated. Streamline-DEAE adsorbent was used for the direct recovery of serine protease from thermal- and non-thermal treatment of crude feedstock. Chromatography is one of the most...

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Main Authors: Amid, Mehrnoush, Abd. Manap, Mohd. Yazid
Format: Article
Published: WFL Publisher 2014
Online Access:http://psasir.upm.edu.my/id/eprint/35177/
http://world-food.net/recovery-of-protease-from-heat-treated-crude-mango-peel-mangifera-indica-cv-chokanan-feedstock-by-expanded-bed-adsorption/
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spelling my.upm.eprints.351772015-12-31T02:09:30Z http://psasir.upm.edu.my/id/eprint/35177/ Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption Amid, Mehrnoush Abd. Manap, Mohd. Yazid The purification of serine protease from crude mango peel feedstock using heat-treated expanded bed adsorption was investigated. Streamline-DEAE adsorbent was used for the direct recovery of serine protease from thermal- and non-thermal treatment of crude feedstock. Chromatography is one of the most important separation techniques, which has been extensively used for the isolation and purification of proteins. Expanded bed adsorption (EBA) has been demonstrated as an efficient technology for separation and purification of proteins directly from unclarified feedstock. Expanded bed chromatography matrices are also available with various ligands and have been successfully applied in many capture chromatographies. In the study, the purification factor and yield of non-thermal treatment of feedstock were 5.8 and 75%, respectively. Heat-treated crude feedstock at 50°C resulted in 2 and 1.2 fold increases in purification factor and yield of serine protease, respectively, compared with that purified from non-heat-treated feedstock. Heating was found to be an important factor in reducing the level of contaminant proteins, therefore non-specific interaction between contaminant and adsorbent was decreased. Dynamic binding capacity and equilibrium adsorption isotherm were increased using heat treatment and enzyme activity assay indicated that the heat treatment had no negative effect on activity of serine protease. WFL Publisher 2014-04 Article PeerReviewed Amid, Mehrnoush and Abd. Manap, Mohd. Yazid (2014) Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption. Journal of Food, Agriculture and Environment, 12 (2). pp. 124-127. ISSN 1459-0255; ESSN: 1459-0263 http://world-food.net/recovery-of-protease-from-heat-treated-crude-mango-peel-mangifera-indica-cv-chokanan-feedstock-by-expanded-bed-adsorption/
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The purification of serine protease from crude mango peel feedstock using heat-treated expanded bed adsorption was investigated. Streamline-DEAE adsorbent was used for the direct recovery of serine protease from thermal- and non-thermal treatment of crude feedstock. Chromatography is one of the most important separation techniques, which has been extensively used for the isolation and purification of proteins. Expanded bed adsorption (EBA) has been demonstrated as an efficient technology for separation and purification of proteins directly from unclarified feedstock. Expanded bed chromatography matrices are also available with various ligands and have been successfully applied in many capture chromatographies. In the study, the purification factor and yield of non-thermal treatment of feedstock were 5.8 and 75%, respectively. Heat-treated crude feedstock at 50°C resulted in 2 and 1.2 fold increases in purification factor and yield of serine protease, respectively, compared with that purified from non-heat-treated feedstock. Heating was found to be an important factor in reducing the level of contaminant proteins, therefore non-specific interaction between contaminant and adsorbent was decreased. Dynamic binding capacity and equilibrium adsorption isotherm were increased using heat treatment and enzyme activity assay indicated that the heat treatment had no negative effect on activity of serine protease.
format Article
author Amid, Mehrnoush
Abd. Manap, Mohd. Yazid
spellingShingle Amid, Mehrnoush
Abd. Manap, Mohd. Yazid
Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption
author_facet Amid, Mehrnoush
Abd. Manap, Mohd. Yazid
author_sort Amid, Mehrnoush
title Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption
title_short Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption
title_full Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption
title_fullStr Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption
title_full_unstemmed Recovery of protease from heat-treated crude mango peel (Mangifera indica cv. Chokanan) feedstock by expanded bed adsorption
title_sort recovery of protease from heat-treated crude mango peel (mangifera indica cv. chokanan) feedstock by expanded bed adsorption
publisher WFL Publisher
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/35177/
http://world-food.net/recovery-of-protease-from-heat-treated-crude-mango-peel-mangifera-indica-cv-chokanan-feedstock-by-expanded-bed-adsorption/
_version_ 1643831375814459392
score 13.211869

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