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Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells

Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Irani...

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Main Authors: Nami, Yousef, Abdullah, Norhafizah, Haghshenas, Babak, Awang Biak, Dayang Radiah, Rosli, Rozita, Khosroushahi, Ahmad Yari
Format: Article
Published: Elsevier 2014
Online Access:http://psasir.upm.edu.my/id/eprint/35065/
http://www.sciencedirect.com/science/article/pii/S107599641400050X
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spelling my.upm.eprints.350652015-12-29T07:51:51Z http://psasir.upm.edu.my/id/eprint/35065/ Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells Nami, Yousef Abdullah, Norhafizah Haghshenas, Babak Awang Biak, Dayang Radiah Rosli, Rozita Khosroushahi, Ahmad Yari Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Iranian women. The L. acidophilus 36YL strain was identified using 16S rDNA gene sequencing and characterized by biochemical methodologies, such as antibiotics susceptibility, antimicrobial activity, and acid and bile resistance. The bioactivity of the secretion of this strain on four human cancer cell lines (AGS, HeLa, MCF-7, and HT-29) and one normal cell line (HUVEC) was evaluated by cytotoxicity assay and apoptosis analysis. This newly isolated strain was found to exhibit notable probiotic properties, such as admirable antibiotic susceptibility, good antimicrobial activity, and favorable resistance to acid and bile salt. The results of bioactivity assessment demonstrated acceptable anticancer effects on the four tested cancer cell lines and negligible side effects on the assayed normal cell line. Our findings revealed that the anticancer effect of L. acidophilus 36YL strain secretions depends on the induction of apoptosis in cancer cells. L. acidophilus 36YL strain is considered as a nutraceutical alternative or a topical medication with a potential therapeutic index because of the absence of cytotoxicity to normal cells, but effective toxicity to cancer cell lines. Elsevier 2014-08 Article PeerReviewed Nami, Yousef and Abdullah, Norhafizah and Haghshenas, Babak and Awang Biak, Dayang Radiah and Rosli, Rozita and Khosroushahi, Ahmad Yari (2014) Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells. Anaerobe, 28. pp. 29-36. ISSN 1075-9964; ESSN: 1095-8274 http://www.sciencedirect.com/science/article/pii/S107599641400050X 10.1016/j.anaerobe.2014.04.012
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Lactobacillus acidophilus is categorized as a probiotic strain because of its beneficial effects in human health and prevention of disease transmission. This study is aimed to characterize the probiotic potential of L. acidophilus 36YL originally isolated from the vagina of healthy and fertile Iranian women. The L. acidophilus 36YL strain was identified using 16S rDNA gene sequencing and characterized by biochemical methodologies, such as antibiotics susceptibility, antimicrobial activity, and acid and bile resistance. The bioactivity of the secretion of this strain on four human cancer cell lines (AGS, HeLa, MCF-7, and HT-29) and one normal cell line (HUVEC) was evaluated by cytotoxicity assay and apoptosis analysis. This newly isolated strain was found to exhibit notable probiotic properties, such as admirable antibiotic susceptibility, good antimicrobial activity, and favorable resistance to acid and bile salt. The results of bioactivity assessment demonstrated acceptable anticancer effects on the four tested cancer cell lines and negligible side effects on the assayed normal cell line. Our findings revealed that the anticancer effect of L. acidophilus 36YL strain secretions depends on the induction of apoptosis in cancer cells. L. acidophilus 36YL strain is considered as a nutraceutical alternative or a topical medication with a potential therapeutic index because of the absence of cytotoxicity to normal cells, but effective toxicity to cancer cell lines.
format Article
author Nami, Yousef
Abdullah, Norhafizah
Haghshenas, Babak
Awang Biak, Dayang Radiah
Rosli, Rozita
Khosroushahi, Ahmad Yari
spellingShingle Nami, Yousef
Abdullah, Norhafizah
Haghshenas, Babak
Awang Biak, Dayang Radiah
Rosli, Rozita
Khosroushahi, Ahmad Yari
Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells
author_facet Nami, Yousef
Abdullah, Norhafizah
Haghshenas, Babak
Awang Biak, Dayang Radiah
Rosli, Rozita
Khosroushahi, Ahmad Yari
author_sort Nami, Yousef
title Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells
title_short Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells
title_full Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells
title_fullStr Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells
title_full_unstemmed Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells
title_sort probiotic potential and biotherapeutic effects of newly isolated vaginal lactobacillus acidophilus 36yl strain on cancer cells
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/35065/
http://www.sciencedirect.com/science/article/pii/S107599641400050X
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score 13.211869

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