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Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates

Aims: This study investigates the antagonistic effects of the probiotic strains Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 against vulvovaginal candidiasis (VVC)-causing Candida glabrata. Methods and Results: Growth inhibitory activities of Lact. rhamnosus GR-1 and Lact. reuteri R...

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Main Authors: Chew, Shu Yih, Cheah, Yoke Kqueen, Seow, Heng Fong, Sandai, Doblin, Than, Leslie Thian Lung
Format: Article
Published: The Society for Applied Microbiology 2015
Online Access:http://psasir.upm.edu.my/id/eprint/35064/
http://onlinelibrary.wiley.com/doi/10.1111/jam.12772/abstract
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spelling my.upm.eprints.350642016-02-10T08:09:54Z http://psasir.upm.edu.my/id/eprint/35064/ Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates Chew, Shu Yih Cheah, Yoke Kqueen Seow, Heng Fong Sandai, Doblin Than, Leslie Thian Lung Aims: This study investigates the antagonistic effects of the probiotic strains Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 against vulvovaginal candidiasis (VVC)-causing Candida glabrata. Methods and Results: Growth inhibitory activities of Lact. rhamnosus GR-1 and Lact. reuteri RC-14 strains against C. glabrata were demonstrated using a spot overlay assay and a plate-based microtitre assay. In addition, these probiotic lactobacilli strains also exhibited potent candidacidal activity against C. glabrata, as demonstrated by a LIVE/DEAD yeast viability assay performed using confocal laser scanning microscopy. The metabolic activities of all C. glabrata strains were completely shut down in response to the challenges by the probiotic lactobacilli strains. In addition, both probiotic lactobacilli strains exhibited strong autoaggregation and coaggregation phenotypes in the presence of C. glabrata, which indicate that these lactobacilli strains may exert their probiotic effects through the formation of aggregates and, thus the consequent prevention of colonization by C. glabrata. Conclusions: Probiotic Lact. rhamnosus GR-1 and Lact. reuteri RC-14 strains exhibited potent antagonistic activities against all of the tested C. glabrata strains. These lactobacilli exhibited antifungal effects, including those attributed to their aggregation abilities, and their presence caused the cessation of growth and eventual cell death of C. glabrata. Significance and Impact of the Study: This is the first study to report on the antagonistic effects of these probiotic lactobacilli strains against the non-Candida albicans Candida (NCAC) species C. glabrata. The Society for Applied Microbiology 2015-05 Article PeerReviewed Chew, Shu Yih and Cheah, Yoke Kqueen and Seow, Heng Fong and Sandai, Doblin and Than, Leslie Thian Lung (2015) Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates. Journal of Applied Microbiology, 118 (5). pp. 1180-1190. ISSN 1364-5072; ESSN: 1365-2672 http://onlinelibrary.wiley.com/doi/10.1111/jam.12772/abstract 10.1111/jam.12772
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Aims: This study investigates the antagonistic effects of the probiotic strains Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 against vulvovaginal candidiasis (VVC)-causing Candida glabrata. Methods and Results: Growth inhibitory activities of Lact. rhamnosus GR-1 and Lact. reuteri RC-14 strains against C. glabrata were demonstrated using a spot overlay assay and a plate-based microtitre assay. In addition, these probiotic lactobacilli strains also exhibited potent candidacidal activity against C. glabrata, as demonstrated by a LIVE/DEAD yeast viability assay performed using confocal laser scanning microscopy. The metabolic activities of all C. glabrata strains were completely shut down in response to the challenges by the probiotic lactobacilli strains. In addition, both probiotic lactobacilli strains exhibited strong autoaggregation and coaggregation phenotypes in the presence of C. glabrata, which indicate that these lactobacilli strains may exert their probiotic effects through the formation of aggregates and, thus the consequent prevention of colonization by C. glabrata. Conclusions: Probiotic Lact. rhamnosus GR-1 and Lact. reuteri RC-14 strains exhibited potent antagonistic activities against all of the tested C. glabrata strains. These lactobacilli exhibited antifungal effects, including those attributed to their aggregation abilities, and their presence caused the cessation of growth and eventual cell death of C. glabrata. Significance and Impact of the Study: This is the first study to report on the antagonistic effects of these probiotic lactobacilli strains against the non-Candida albicans Candida (NCAC) species C. glabrata.
format Article
author Chew, Shu Yih
Cheah, Yoke Kqueen
Seow, Heng Fong
Sandai, Doblin
Than, Leslie Thian Lung
spellingShingle Chew, Shu Yih
Cheah, Yoke Kqueen
Seow, Heng Fong
Sandai, Doblin
Than, Leslie Thian Lung
Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates
author_facet Chew, Shu Yih
Cheah, Yoke Kqueen
Seow, Heng Fong
Sandai, Doblin
Than, Leslie Thian Lung
author_sort Chew, Shu Yih
title Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates
title_short Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates
title_full Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates
title_fullStr Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates
title_full_unstemmed Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates
title_sort probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing candida glabrata isolates
publisher The Society for Applied Microbiology
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/35064/
http://onlinelibrary.wiley.com/doi/10.1111/jam.12772/abstract
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score 13.211869

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