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Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications

Here, we explore the use of two- and three-dimensional scaffolds of multiwalled-carbon nanotubes (MWNTs) for hepatocyte cell culture. Our objective is to study the use of these scaffolds in liver tissue engineering and drug discovery. In our experiments, primary rat hepatocytes, the parenchymal (mai...

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Main Authors: Che Abdullah, Che Azurahanim, Azad, Chihye Lewis, Robles, Raquel Ovalle, Fang, Shaoli, Lima, Marcio D., Lepro, Xavier, Collins, Steve, Baughman, Ray H., Dalton, Alan B., Plant, Nick J., Sear, Richard P.
Format: Article
Language:English
Published: American Chemical Society 2014
Online Access:http://psasir.upm.edu.my/id/eprint/35051/1/Primary%20liver%20cells%20cultured%20on%20carbon%20nanotube%20substrates%20for%20liver%20tissue%20engineering%20and%20drug%20discovery%20applications.pdf
http://psasir.upm.edu.my/id/eprint/35051/
http://pubs.acs.org/doi/abs/10.1021/am5018489
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spelling my.upm.eprints.350512016-11-14T08:15:43Z http://psasir.upm.edu.my/id/eprint/35051/ Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications Che Abdullah, Che Azurahanim Azad, Chihye Lewis Robles, Raquel Ovalle Fang, Shaoli Lima, Marcio D. Lepro, Xavier Collins, Steve Baughman, Ray H. Dalton, Alan B. Plant, Nick J. Sear, Richard P. Here, we explore the use of two- and three-dimensional scaffolds of multiwalled-carbon nanotubes (MWNTs) for hepatocyte cell culture. Our objective is to study the use of these scaffolds in liver tissue engineering and drug discovery. In our experiments, primary rat hepatocytes, the parenchymal (main functional) cell type in the liver, were cultured on aligned nanogrooved MWNT sheets, MWNT yarns, or standard 2-dimensional culture conditions as a control. We find comparable cell viability between all three culture conditions but enhanced production of the hepatocyte-specific marker albumin for cells cultured on MWNTs. The basal activity of two clinically relevant cytochrome P450 enzymes, CYP1A2 and CYP3A4, are similar on all substrates, but we find enhanced induction of CYP1A2 for cells on the MWNT sheets. Our data thus supports the use of these substrates for applications including tissue engineering and enhancing liver-specific functions, as well as in in vitro model systems with enhanced predictive capability in drug discovery and development. American Chemical Society 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35051/1/Primary%20liver%20cells%20cultured%20on%20carbon%20nanotube%20substrates%20for%20liver%20tissue%20engineering%20and%20drug%20discovery%20applications.pdf Che Abdullah, Che Azurahanim and Azad, Chihye Lewis and Robles, Raquel Ovalle and Fang, Shaoli and Lima, Marcio D. and Lepro, Xavier and Collins, Steve and Baughman, Ray H. and Dalton, Alan B. and Plant, Nick J. and Sear, Richard P. (2014) Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications. ACS Applied Materials & Interfaces, 6 (13). pp. 10373-10380. ISSN 1944-8244; ESSN: 1944-8252 http://pubs.acs.org/doi/abs/10.1021/am5018489 10.1021/am5018489
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Here, we explore the use of two- and three-dimensional scaffolds of multiwalled-carbon nanotubes (MWNTs) for hepatocyte cell culture. Our objective is to study the use of these scaffolds in liver tissue engineering and drug discovery. In our experiments, primary rat hepatocytes, the parenchymal (main functional) cell type in the liver, were cultured on aligned nanogrooved MWNT sheets, MWNT yarns, or standard 2-dimensional culture conditions as a control. We find comparable cell viability between all three culture conditions but enhanced production of the hepatocyte-specific marker albumin for cells cultured on MWNTs. The basal activity of two clinically relevant cytochrome P450 enzymes, CYP1A2 and CYP3A4, are similar on all substrates, but we find enhanced induction of CYP1A2 for cells on the MWNT sheets. Our data thus supports the use of these substrates for applications including tissue engineering and enhancing liver-specific functions, as well as in in vitro model systems with enhanced predictive capability in drug discovery and development.
format Article
author Che Abdullah, Che Azurahanim
Azad, Chihye Lewis
Robles, Raquel Ovalle
Fang, Shaoli
Lima, Marcio D.
Lepro, Xavier
Collins, Steve
Baughman, Ray H.
Dalton, Alan B.
Plant, Nick J.
Sear, Richard P.
spellingShingle Che Abdullah, Che Azurahanim
Azad, Chihye Lewis
Robles, Raquel Ovalle
Fang, Shaoli
Lima, Marcio D.
Lepro, Xavier
Collins, Steve
Baughman, Ray H.
Dalton, Alan B.
Plant, Nick J.
Sear, Richard P.
Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
author_facet Che Abdullah, Che Azurahanim
Azad, Chihye Lewis
Robles, Raquel Ovalle
Fang, Shaoli
Lima, Marcio D.
Lepro, Xavier
Collins, Steve
Baughman, Ray H.
Dalton, Alan B.
Plant, Nick J.
Sear, Richard P.
author_sort Che Abdullah, Che Azurahanim
title Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
title_short Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
title_full Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
title_fullStr Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
title_full_unstemmed Primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
title_sort primary liver cells cultured on carbon nanotube substrates for liver tissue engineering and drug discovery applications
publisher American Chemical Society
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/35051/1/Primary%20liver%20cells%20cultured%20on%20carbon%20nanotube%20substrates%20for%20liver%20tissue%20engineering%20and%20drug%20discovery%20applications.pdf
http://psasir.upm.edu.my/id/eprint/35051/
http://pubs.acs.org/doi/abs/10.1021/am5018489
_version_ 1643831338446356480
score 13.211869

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