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Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)

Structured lipid such as medium-and long-chain triacylglycerol (MLCT) is claimed to be able to suppress body fat accumulation and be used to manage obesity. Response surface methodology (RSM) with four factors and three levels (+1,0,−1) faced centered composite design (FCCD) was employed for optimiz...

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Main Authors:	Lee, Yee Ying, Tang, Teck Kim, Phuah, Eng Tong, Ab Karim, Nur Azwani, Mohd Alwi, Siti Maslina, Lai, Oi Ming
Format:	Article
Published:	Springer 2014
Online Access:	http://psasir.upm.edu.my/id/eprint/34809/ http://link.springer.com/article/10.1007%2Fs13197-013-1065-0
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spelling	my.upm.eprints.348092015-12-23T02:53:33Z http://psasir.upm.edu.my/id/eprint/34809/ Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM) Lee, Yee Ying Tang, Teck Kim Phuah, Eng Tong Ab Karim, Nur Azwani Mohd Alwi, Siti Maslina Lai, Oi Ming Structured lipid such as medium-and long-chain triacylglycerol (MLCT) is claimed to be able to suppress body fat accumulation and be used to manage obesity. Response surface methodology (RSM) with four factors and three levels (+1,0,−1) faced centered composite design (FCCD) was employed for optimization of the enzymatic interesterification conditions of palm-based MLCT (P-MLCT) production. The effect of the four variables namely: substrate ratio palm kernel oil: palm oil, PKO:PO (40:60–100:0 w/w), temperature (50–70 °C), reaction time (0.5–7.5 h) and enzyme load (5–15 % w/w) on the P-MLCT yield (%) and by products (%) produced were investigated. The responses were determined via acylglycerol composition obtained from high performance liquid chromatography. Well-fitted models were successfully established for both responses: P-MLCT yield (R 2 = 0.9979) and by-products (R 2 = 0.9892). The P-MLCT yield was significantly (P < 0.05) affected by substrate ratio, reaction time and reaction temperature but not enzyme load (P > 0.05). Substrate ratio PKO: PO (100:0 w/w) gave the highest yield of P-MLCT (61 %). Nonetheless, substrate ratio of PKO: PO (90:10w/w) was chosen to improve the fatty acid composition of the P-MLCT. The optimized conditions for substrate ratio PKO: PO (90:10 w/w) was 7.26 h, 50 °C and 5 % (w/w) Lipozyme TLIM lipase, which managed to give 60 % yields of P-MLCT. Up scaled results in stirred tank batch reactor gave similar yields as lab scale. A 20 % increase in P-MLCT yield was obtained via RSM. The effect of enzymatic interesterification on the physicochemical properties of PKO:PO (90:10 w/w) were also studied. Thermoprofile showed that the P-MLCT oil melted below body temperature of 37 °C. Springer 2014 Article PeerReviewed Lee, Yee Ying and Tang, Teck Kim and Phuah, Eng Tong and Ab Karim, Nur Azwani and Mohd Alwi, Siti Maslina and Lai, Oi Ming (2014) Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM). Journal of Food Science and Technology, 52 (2). pp. 685-696. ISSN 0022-1155; ESSN: 0975-8402 http://link.springer.com/article/10.1007%2Fs13197-013-1065-0 10.1007/s13197-013-1065-0
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Structured lipid such as medium-and long-chain triacylglycerol (MLCT) is claimed to be able to suppress body fat accumulation and be used to manage obesity. Response surface methodology (RSM) with four factors and three levels (+1,0,−1) faced centered composite design (FCCD) was employed for optimization of the enzymatic interesterification conditions of palm-based MLCT (P-MLCT) production. The effect of the four variables namely: substrate ratio palm kernel oil: palm oil, PKO:PO (40:60–100:0 w/w), temperature (50–70 °C), reaction time (0.5–7.5 h) and enzyme load (5–15 % w/w) on the P-MLCT yield (%) and by products (%) produced were investigated. The responses were determined via acylglycerol composition obtained from high performance liquid chromatography. Well-fitted models were successfully established for both responses: P-MLCT yield (R 2 = 0.9979) and by-products (R 2 = 0.9892). The P-MLCT yield was significantly (P < 0.05) affected by substrate ratio, reaction time and reaction temperature but not enzyme load (P > 0.05). Substrate ratio PKO: PO (100:0 w/w) gave the highest yield of P-MLCT (61 %). Nonetheless, substrate ratio of PKO: PO (90:10w/w) was chosen to improve the fatty acid composition of the P-MLCT. The optimized conditions for substrate ratio PKO: PO (90:10 w/w) was 7.26 h, 50 °C and 5 % (w/w) Lipozyme TLIM lipase, which managed to give 60 % yields of P-MLCT. Up scaled results in stirred tank batch reactor gave similar yields as lab scale. A 20 % increase in P-MLCT yield was obtained via RSM. The effect of enzymatic interesterification on the physicochemical properties of PKO:PO (90:10 w/w) were also studied. Thermoprofile showed that the P-MLCT oil melted below body temperature of 37 °C.
format	Article
author	Lee, Yee Ying Tang, Teck Kim Phuah, Eng Tong Ab Karim, Nur Azwani Mohd Alwi, Siti Maslina Lai, Oi Ming
spellingShingle	Lee, Yee Ying Tang, Teck Kim Phuah, Eng Tong Ab Karim, Nur Azwani Mohd Alwi, Siti Maslina Lai, Oi Ming Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)
author_facet	Lee, Yee Ying Tang, Teck Kim Phuah, Eng Tong Ab Karim, Nur Azwani Mohd Alwi, Siti Maslina Lai, Oi Ming
author_sort	Lee, Yee Ying
title	Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)
title_short	Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)
title_full	Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)
title_fullStr	Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)
title_full_unstemmed	Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)
title_sort	palm-based medium-and-long-chain triaclyglycerol (p-mlct): production via enzymatic interesterification and optimization using response surface methodology (rsm)
publisher	Springer
publishDate	2014
url	http://psasir.upm.edu.my/id/eprint/34809/ http://link.springer.com/article/10.1007%2Fs13197-013-1065-0
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score	13.211869

Palm-based medium-and-long-chain triaclyglycerol (P-MLCT): production via enzymatic interesterification and optimization using response surface methodology (RSM)

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